Cell-free synthesis of the h-cluster: A model for the in vitro assembly of metalloprotein metal centers

Methods in Molecular Biology

Volumn 1122, Issue , 2014, Pages 49-72

  Kuchenreuther, Jon M ^a   Shiigi, Stacey A ^a   Swartz, James R ^a  

^a NONE

Author keywords

Accessory protein; Cell free; Cofactor; Expression; H cluster; Hydrogenase; In vitro; Purification; Synthetic biology

Indexed keywords

HYDROGENASE; METALLOPROTEIN; CELL EXTRACT; METAL;

ARTICLE; CELL FREE SYSTEM; CELL LYSATE; CHROMATOGRAPHY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ESCHERICHIA COLI; HETEROLOGOUS EXPRESSION; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SYNTHESIS; AFFINITY CHROMATOGRAPHY; ANAEROBIC GROWTH; ANIMAL; BIOCHEMISTRY; CHICKEN; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES;

ESCHERICHIA COLI;

ANAEROBIOSIS; ANIMALS; BIOCHEMISTRY; CELL EXTRACTS; CELL-FREE SYSTEM; CHICKENS; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HYDROGENASE; METALLOPROTEINS; METALS;

EID: 84921357530     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-794-5_5     Document Type: Article

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