Gypenosides attenuate cholesterol-induced DNA damage by inhibiting the production of reactive oxygen species in human umbilical vein endothelial cells

Molecular Medicine Reports

Volumn 11, Issue 4, 2015, Pages 2845-2851

  Quan, Yuan ^a   Yang, Yijun ^a   Wang, Huixing ^a   Shu, Bo ^a   Gong, Qi Hai ^a   Qian, Minzhang ^a  

^a ZUNYI MEDICAL UNIVERSITY   (China)

Author keywords

Atherosclerosis; DNA damage; Gynostemma Pentaphylla (Thunb.) Makino; Gypenosides; Oxidative stress

Indexed keywords

GYPENOSIDE; HISTONE H2AX; REACTIVE OXYGEN METABOLITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE 4; ANTIOXIDANT; CHOLESTEROL; HISTONE; NITRIC OXIDE SYNTHASE; PLANT EXTRACT; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE; XANTHINE OXIDASE;

ANTIOXIDANT ACTIVITY; ARTICLE; ATHEROSCLEROSIS; CONTROLLED STUDY; DNA DAMAGE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HISTONE PHOSPHORYLATION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UMBILICAL VEIN ENDOTHELIAL CELL; WESTERN BLOTTING; CHEMISTRY; DRUG EFFECTS; GENE EXPRESSION REGULATION; GENETICS; GYNOSTEMMA; METABOLISM; OXIDATIVE STRESS;

GYNOSTEMMA PENTAPHYLLUM; RATTUS;

ANTIOXIDANTS; CHOLESTEROL; DNA DAMAGE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GYNOSTEMMA; HISTONES; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; NADPH OXIDASE; NITRIC OXIDE SYNTHASE; OXIDATIVE STRESS; PLANT EXTRACTS; REACTIVE OXYGEN SPECIES; XANTHINE OXIDASE;

EID: 84921345890     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2014.3095     Document Type: Article

References (24)

1. Piao XL and Wu Q: Progressive studies on Gynostemma pentaphyllum. Lishizhen Medicine and Materia Medica Research 21: 1758-1760, 2010.
2. Liu XY, Wei JM and Zhou R: Study on effective components extraction and antioxidant capacities of Gynostemma pentaphyllum Makino. J Anhui Agri Sci 36: 13670-13672, 2008 (In Chinese).
3. Quan Y and Qian MZ: Effect and mechanism of Gypenosides on the inflammatory molecular expression in high-fat induced atherosclerosis rat. Chinese Journal of Integrated Traditional and Western Medicine 30: 403-406, 2010 (In Chinese).
4. Mercer JR, Cheng KK, Figg N, et al.: DNA damage links mitochondrial dysfunction to atherosclerosis and the metabolic syndrome. Circ Res 107: 1021-1031, 2010
5. Botto N, Rizza A, Colombo M.G., et al.: Evidence for DNA damage in patients with coronary artery disease. Mutat Res 493: 23-30, 2001.
6. Andreassi MG and Botto N: DNA damage as a new emerging risk factor in atherosclerosis. Trends Cardiovasc Med 13: 270-275, 2003.
7. Quan Y and Qian MZ: Cholesterol induces endothelial cells injury by increasing production ofreactive oxygen species and activating NF-kB. Chinese J Pathophysiol 26: 1521-1526, 2010 (In Chinese).
8. Wang HX, Yang YJ and Qian MZ: Cholesterol induces DNA damage in HUVECs. Chinese Journal of Biochemistry and Molecular Biology 28: 442-448, 2012 (In Chinese).
9. Liu F, Ren D, Guo D.A., Pan Y., Zhang H and Hu P: Method development for gypenosides fingerprint by high performance liquid chromatography with diode-array detection and the addition of internal standard. Chem Pharm Bull (Tokyo) 56: 389-393, 2008.
10. Mahmoudi M, Mercer J, Bennett M: DNA damage and repair in atherosclerosis. Cardiovasc Res 71: 259-268, 2006.
11. Chen X: Cardiovascular protection by ginsenosides and their nitric oxide releasing action. Clin Exp Pharmacol Physiol 23: 728-732, 1996.
12. Thompson JA and Hess ML: The oxygen free radical system: a fundamental mechanism in production of myocardial necrosis. Prog Cardiovasc Dis 28: 449-462, 1986.
13. 3 on electric and contractile activities of normal and damaged cultured myocardiocytes. Acta Pharmacol Sin 13: 403-406, 1992.
14. Rogakou EP, Pilch DR, Orr A.H., Ivanova VS and Bonner WM: DNA double-stranded breaks induce histone H2AX phosphory-lation on serine 139. J Biol Chem 273: 5858-5868, 1998.
15. Ago T, Kitazono T, Ooboshi H., et al.: NOX4 as the major catalytic component of anendothelial NAD(P)H oxidase. Circulation 109: 227-233, 2004.
16. Yamagishi S, Nakamura K, Ueda S., Kato S and Imaizumi T: Pigment epithelium-derived factor (PEDF) blocks angiotensin II signaling in endothelial cells via suppression of NADPH oxidase: a novel anti-oxidative mechanism of PEDF. Cell Tissue Res 320: 437-445, 2005.
17. Guzik TJ and Harrison DG: Vascular NADPH oxidases as drug targets for novel antioxidant strategies. Drug Discov Today 11: 524-533 2006.
18. Alderton WK, Cooper CE and Knowles RG: Nitricoxide synthases: structure, function and inhibition. Biochem J 357: 593-615, 2001.
19. Zhou L, Xu Y P, Wei Y, et al.: Effects of gypenosides on blood lipid and lipid peroxidation in experimental hyperlipidemia rats. Chinese Journal of Applied Physiology 13: 228-232, 2005.
20. Yang YH, Yang J and Jiang QH: Hypolipidemic effect of gypenosides in experimentally induced hypercholesterolemic rats. Lipids Health Dis 12: 154, 2013.
21. 2 sensing is preserved in mice lacking the gp91 Phox subunit of NADPH oxidase. Proc Natl Acad Sci USA 96: 7944-7949, 1999.
22. Olszanecki R, Kozlovski VI, Chłopicki S and Gryglewski RJ: Paradoxical augmentation of bradykinin-induced vasodilatation by xanthine/xanthine oxidase-derived free radicals in isolated guinea pig heart. J Physiol Pharmacol 53: 689-699, 2002.
23. Geiszt M, Kopp JB, Várnai P and Leto TL: Identification of Renox, an NAD(P)H oxidase in kidney. Proc Natl Acad Sci USA 97: 8010-8014, 2000.
24. Dijkstra G, Moshage H, van Dullemen HM, et al.: Expression of nitric oxide synthases, and formation of nitrotyrosine and reactive oxygen species in inflammatory bowel disease. J Pathol 186: 416-421, 1998.