Isolation of herpes simplex virus nucleocapsid DNA

Methods in Molecular Biology

Volumn 1144, Issue , 2014, Pages 31-41

  Szpara, Moriah ^a  

^a NONE

Author keywords

Centrifugation; Freon ; Genomes; Nonionic detergents; Nucleocapsid DNA

Indexed keywords

HISTONE; VIRUS DNA; CAPSID PROTEIN;

ARTICLE; CENTRIFUGATION; CYTOPATHOGENIC EFFECT; DISSOLUTION; HERPES SIMPLEX VIRUS; HOST CELL; NONHUMAN; PRIORITY JOURNAL; VIRUS GENOME; VIRUS ISOLATION; VIRUS NUCLEOCAPSID; ANIMAL; CHEMISTRY; CHLOROCEBUS AETHIOPS; GENETICS; HERPES SIMPLEX VIRUS 1; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR BIOLOGY; PROCEDURES; VERO CELL LINE; VIRION;

ANIMALS; CAPSID PROTEINS; CERCOPITHECUS AETHIOPS; DNA, VIRAL; GENOME, VIRAL; HERPESVIRUS 1, HUMAN; HUMANS; MOLECULAR BIOLOGY; NUCLEOCAPSID; VERO CELLS; VIRION;

EID: 84921288217     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0428-0_3     Document Type: Article

References (10)

1. Roizman B, Knipe DM (2001) Herpes simplex viruses and their replication. In: Knipe DM, Howley PM (eds) Fields virology, 4th edn. Lippincott Williams & Wilkins, Philadelphia, PA, pp 2399-2460.
2. Szpara ML, Tafuri YR, Enquist LW (2011) Preparation of viral DNA from nucleocapsids. J Vis Exp 54: 3151. doi: 10. 3791/3151.
3. Enquist LW, Madden MJ, Schiop-Stanley P, Vande Woude GF (1979) Cloning of herpes simplex type 1 DNA fragments in a bacteriophage lambda vector. Science 203: 541-544.
4. Sottong P, Harris R, Graham B, Rupp B, Bell C et al (1976) Purification of herpesvirus nucleocapsids by fluorocarbon extraction. Microbios 16: 105-110.
5. Smith GA, Enquist LW (1999) Construction and transposon mutagenesis in Escherichia coli of a full-length infectious clone of pseudorabies virus, an alphaherpesvirus. J Virol 73: 6405-6414.
6. Szpara ML, Tafuri YR, Parsons L, Shamim SR, Verstrepen KJ et al (2011) A wide extent of interstrain diversity in virulent and vaccine strains of alphaherpesviruses. PLoS Pathog 7: e1002282.
7. Mendez II, Hermann LL, Hazelton PR, Coombs KM (2000) A comparative analysis of freon substitutes in the purification of reovirus and calicivirus. J Virol Methods 90: 59-67.
8. Leggitt PR, Jaykus LA (2000) Detection methods for human enteric viruses in representative foods. J Food Prot 63: 1738-1744.
9. Mignotte B, Maul A, Schwartzbrod L (1999) Comparative study of techniques used to recover viruses from residual urban sludge. J Virol Methods 78: 71-80.
10. Traore O, Arnal C, Mignotte B, Maul A, Laveran H et al (1998) Reverse transcriptase PCR detection of astrovirus, hepatitis A virus, and poliovirus in experimentally contaminated mussels: comparison of several extraction and concentration methods. Appl Environ Microbiol 64: 3118-3122.