Using homogeneous primary neuron cultures to study fundamental aspects of HSV-1 latency and reactivation

Methods in Molecular Biology

Volumn 1144, Issue , 2014, Pages 167-179

  Kim, Ju Youn ^a   Shiflett, Lora A ^a   Linderman, Jessica A ^a   Mohr, Ian ^a   Wilson, Angus C ^a  

^a NONE

Author keywords

HSV 1; In vitro system; Latency; Lentiviral delivery; Reactivation; RNA interference; SCG neuron culture

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EMBRYO; EXPLANT; HERPES SIMPLEX VIRUS 1; HERPES VIRUS INFECTION; HOST; IMMUNOCOMPETENT CELL; LENTIVIRINAE; NERVE CELL; NERVE CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; RAT; RAT EMBRYO; SUPERIOR CERVICAL GANGLION; VIRUS LATENCY; VIRUS REACTIVATION; ANIMAL; CYTOLOGY; DRUG EFFECTS; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; MOLECULAR BIOLOGY; PRIMARY CELL CULTURE; PROCEDURES; VIRUS ACTIVATION;

ANIMALS; HERPESVIRUS 1, HUMAN; HUMANS; MOLECULAR BIOLOGY; NEURONS; PRIMARY CELL CULTURE; VIRUS ACTIVATION; VIRUS LATENCY;

EID: 84921273108     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0428-0_11     Document Type: Article

References (13)

1. Wilson AC, Mohr I (2012) A cultured affair: HSV latency and reactivation in neurons. Trends Microbiol 20:604–611
2. Wagner EK, Bloom DC (1997) Experimental investigation of herpes simplex virus latency. Clin Microbiol Rev 10:419–443
3. Kobayashi M, Wilson AC, Chao MV et al (2012) Control of viral latency in neurons by axonal mTOR signaling and the 4E-BP translation repressor. Genes Dev 26:1527–1532
4. Camarena V, Kobayashi M, Kim JY et al (2010) Nature and duration of growth factor signaling through receptor tyrosine kinases regulates HSV-1 latency in neurons. Cell Host Microbe 8:320–330
5. Wilcox CL, Smith RL, Freed CR et al (1990) Nerve growth factor-dependence of herpes simplex virus latency in peripheral sympathetic and sensory neurons in vitro. J Neurosci 10: 1268–1275
6. Wilcox CL, Johnson EM (1988) Characterization of nerve growth factor- dependent herpes simplex virus latency in neurons in vitro. J Virol 62:393–399
7. Roehm PC, Camarena V, Nayak S et al (2011) Cultured vestibular ganglion neurons demonstrate latent HSV1 reactivation. Laryngoscope 121:2268–2275
8. Kuhn MA, Nayak S, Camarena V et al (2012) A cell culture model of facial palsy resulting from reactivation of latent herpes simplex type 1. Otol Neurotol 33:87–92
9. Warren KG, Brown SM, Wroblewska Z et al (1978) Isolation of latent herpes simplex virus from the superior cervical and vagus ganglions of human beings. N Engl J Med 298: 1068–1069
10. Price RW, Katz BJ, Notkins AL (1975) Latent infection of the peripheral ANS with herpes simplex virus. Nature 257:686–688
11. Kim JY, Mandarino A, Chao MV et al (2012) Transient reversal of episome silencing precedes VP16-dependent transcription during reactivation of latent HSV-1 in neurons. PLoS Pathog 8: 1002540
12. Harlow E, Lane D (eds) (1988) Antibodies. A laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, NY
13. Sambrook J, Fritsch EF, Maniatis T (eds) (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, NY