Evaluation of Bacillus subtilis R0179 on gastrointestinal viability and general wellness: A randomised, double-blind, placebo-controlled trial in healthy adults

Beneficial Microbes

Volumn 6, Issue 1, 2015, Pages 19-27

  Hanifi, A ^a   Culpepper, T ^b   Mai, V ^b   Anand, A ^b   Ford, A L ^a   Ukhanova, M ^b   Christman, M ^c   Tompkins, T A ^d   Dahl, W J ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

^c MCC Statistical Consulting LLC   (United States)

^d LALLEMAND SAS   (France)

Author keywords

Gastrointestinal symptoms; GSRS; Microbiota; Probiotic; Viability

Indexed keywords

PROBIOTIC AGENT; RNA 16S; BACTERIAL DNA; PLACEBO; RIBOSOME DNA;

ABDOMINAL PAIN; ADULT; ARTICLE; BACILLUS SUBTILIS; BACTERIAL COUNT; BACTERIAL SURVIVAL; BACTERIAL VIABILITY; BACTEROIDETES; COLONY FORMING UNIT F; CONSTIPATION; CONTROLLED STUDY; DIARRHEA; FATIGUE; FEMALE; FIRMICUTES; GASTROINTESTINAL REFLUX; GASTROINTESTINAL SYMPTOM; GASTROINTESTINAL TRANSIT; HUMAN; HUMAN EXPERIMENT; MALE; MIDDLE AGED; NONHUMAN; NORMAL HUMAN; QUESTIONNAIRE; RANDOMIZED CONTROLLED TRIAL; RATING SCALE; RUMINOCOCCUS; SCORING SYSTEM; TREATMENT RESPONSE; ADVERSE DRUG REACTION; CHEMISTRY; DNA SEQUENCE; DOUBLE BLIND PROCEDURE; FECES; GASTROINTESTINAL TRACT; GENETICS; MICROBIAL VIABILITY; MICROBIOLOGY; ORAL DRUG ADMINISTRATION; PHYSIOLOGY;

ADMINISTRATION, ORAL; ADULT; BACILLUS SUBTILIS; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; DNA, RIBOSOMAL; DOUBLE-BLIND METHOD; DRUG-RELATED SIDE EFFECTS AND ADVERSE REACTIONS; FECES; GASTROINTESTINAL TRACT; HUMANS; MICROBIAL VIABILITY; PLACEBOS; PROBIOTICS; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

EID: 84920964391     PISSN: 18762883     EISSN: 18762891     Source Type: Journal    
DOI: 10.3920/BM2014.0031     Document Type: Article

References (17)

1. Bull, F.C., Maslin, T.S. and Armstrong, T., 2009. Global physical activity questionnaire (GPAQ): nine country reliability and validity study. Journal of Physical Activity and Health 6: 790-804.
2. Caporaso, J.G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F.D., Costello, E.K., Fierer, N., Pena, A.G., Goodrich, J.K., Gordon, J.I., Huttley, G.A., Kelley, S.T., Knights, D., Koenig, J.E., Ley, R.E., Lozupone, C.A., McDonald, D., Muegge, B.D., Pirrung, M., Reeder, J., Sevinsky, J.R., Turnbaugh, P.J., Walters, W.A., Widmann, J., Yatsunenko, T., Zaneveld, J. and Knight, R., 2010. QIIME allows analysis of high-throughput community sequencing data. Nature Methods 7: 335-336.
3. Casula, G. and Cutting, S.M., 2002. Bacillus probiotics: spore germination in the gastrointestinal tract. Applied and Environmental Microbiology 68: 2344-2352.
4. Chen, L., Liu, L., Hu, Z. and Lu, H., 2012. Meta-analysis of the therapeutic effects of Medilac-S on IBS. China Pharmacy 23: 2857-2860.
5. Cole, J.R., Wang, Q., Cardenas, E., Fish, J., Chai, B., Farris, R.J., Kulam-Syed-Mohideen, A.S., McGarrell, D.M., Marsh, T., Garrity, G.M. and Tiedje, J.M., 2009. The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucleic Acids Research 37: D141-D145.
6. Cutting, S.M., 2011. Bacillus probiotics. Food Microbiology 28: 214-220.
7. Dimenas, E., Glise, H., Hallerback, B., Hernqvist, H., Svedlund, J. and Wiklund, I., 1993. Quality of life in patients with upper gastrointestinal symptoms. An improved evaluation of treatment regimens? Scandinavian Journal of Gastroenterology 28: 681-687.
8. Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO working group on drafting guidelines for the evaluation of probiotics in food. Available at: ftp://ftp.fao.org/es/esn/food/wgreport2.pdf.
9. Hong, H.A., Khaneja, R., Tam, N.M., Cazzato, A., Tan, S., Urdaci, M., Brisson, A., Gasbarrini, A., Barnes, I. and Cutting, S.M., 2009b. Bacillus subtilis isolated from the human gastrointestinal tract. Research in Microbiology 160: 134-143.
10. Hong, H.A., To, E., Fakhry, S., Baccigalupi, L., Ricca, E. and Cutting, S.M., 2009a. Defining the natural habitat of Bacillus spore-formers. Research in Microbiology 160: 375-379.
11. Hu, Z., Chen, L., Teng, F., Liu, L. and Lu, H., 2013. Systematic review of combination of Medilac-S and conventional treatment for ulcerative colitis. China Pharmaceuticals 22: 3-7.
12. Miller, D.N., Bryant, J.E., Madsen, E.L. and Ghiorse, W.C., 1999. Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples. Applied Environmental Microbiology 65: 4715-4724.
13. Nicholson, W.L., 2002. Roles of Bacillus endospores in the environment. Cellular Molecular Life Sciences 59: 410-416.
14. Liu, L., Chen, L., Hu, Z. and Lu, H., 2012. Systematic review of combined Bacillus subtilis and Enterococcus faecium for prevention and treatment of antibiotic-associated diarrhea. Medical Journal of National Defending Forces in Southwest China 22: 1196-1200.
15. Sun, Y., Cai, Y., Liu, L., Yu, F., Farrell, M.L., McKendree, W. and Farmerie, W., 2009. ESPRIT: estimating species richness using large collections of 16S rRNA pyrosequences. Nucleic Acids Research 37: e76.
16. Svedlund, J., Sjodin, I. and Dotevall, G., 1988. GSRS - a clinical rating scale for gastrointestinal symptoms in patients with irritable bowel syndrome and peptic ulcer disease. Digestive Diseases and Sciences 33: 129-134.
17. Tompkins, T.A., Xu, X. and Ahmarani, J., 2010. A comprehensive review of post-market clinical studies performed in adults with an Asian probiotic formulation. Beneficial Microbes 1: 93-106.