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84920763846
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note
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In that case, the amino group underwent cleavage of the trifluoroacetyl group and further acetyl protection. The acetyl group could not be removed during final deprotection.
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46
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84920763845
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note
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Some other protecting groups were tested before, such as the benzoyl and the acetyl group. Unfortunately, the deprotection strategies always failed.
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47
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84920763844
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note
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Buffer CHES pH 9 was used according to previous studies in the group (see Refs. [12] and [18]). Nevertheless, pH 7 and pH 8 conditions have also been studied (with different buffers). The results are similar to the experiments performed at pH 9. The denaturing/renaturing behavior of duplex D1d under variation of the pH conditions is presented in Figures S2 and S3 as well as Table S4 in the Supporting Information.
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48
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84920763843
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note
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Melting point experiments to study the selectivity of the To towards the canonical bases have been performed. The results are shown in Figure S1 in the Supporting information.
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49
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84920763842
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note
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The feature of hysteresis as well as the same tendency of rate dependence are observed under different pH conditions. However, at pH 7 and pH 8 the hysteresis is always smaller (independently of the buffer employed). The maximum value is achieved at pH 9. The results are shown in Figures S2 and S3 in the Supporting Information.
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