Free fatty acids enhance the oxidative damage induced by ethanol metabolism in an in vitro model

Food and Chemical Toxicology

Volumn 76, Issue , 2015, Pages 109-115

  Hernández, Ileana ^a   Domínguez Pérez, Mayra ^a   Bucio, Leticia ^a,b   Souza, Verónica ^a,b   Miranda, Roxana U ^a,b   Clemens, Dahn L ^c   Gomez Quiroz, Luis Enrique ^a,b   Gutiérrez Ruiz, María Concepción ^a,b  

^a DEPARTAMENTO DE QUÍMICA   (Mexico)

^b Red Fisiopatología de Enferemedades Hepáticas PROMEP SEP   (Mexico)

^c UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

Ethanol; Hepatocytes; Oxidative stress; Steatosis; VL 17A

Indexed keywords

ALCOHOL; ALCOHOL DEHYDROGENASE; COPPER ZINC SUPEROXIDE DISMUTASE; CYTOCHROME P450 2E1; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; GLUTATHIONE PEROXIDASE; OLEIC ACID; PALMITIC ACID; REACTIVE OXYGEN METABOLITE; ANTIOXIDANT; DIACETYLDICHLOROFLUORESCEIN; FATTY ACID; FLUORESCEIN DERIVATIVE; TRIACYLGLYCEROL;

ALCOHOL METABOLISM; APOPTOSIS; ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; FATTY LIVER; IN VITRO STUDY; LIPID STORAGE; LIVER CELL DAMAGE; OXIDATION; OXIDATIVE STRESS; PROTEIN EXPRESSION; BIOSYNTHESIS; DRUG EFFECTS; GENETICS; HEPG2 CELL LINE; HUMAN; LIVER CELL; METABOLISM;

ALCOHOL DEHYDROGENASE; ANTIOXIDANTS; APOPTOSIS; CYTOCHROME P-450 CYP2E1; ETHANOL; FATTY ACIDS, NONESTERIFIED; FLUORESCEINS; GLUTAMATE-CYSTEINE LIGASE; GLUTATHIONE; HEP G2 CELLS; HEPATOCYTES; HUMANS; OXIDATIVE STRESS; REACTIVE OXYGEN SPECIES; TRIGLYCERIDES;

EID: 84920749415     PISSN: 02786915     EISSN: 18736351     Source Type: Journal    
DOI: 10.1016/j.fct.2014.12.005     Document Type: Article

References (30)

1. Begriche K., Massart J., Robin M.A., Bonnet F., Fromenty B. Mitochondrial adaptations and dysfunctions in nonalcoholic fatty liver disease. Hepatology 2013, 58:1497-1507.
2. Chen Y., Dong H., Thompson D.C., Shertzer H.G., Nebert D.W., Vasiliou V. Glutathione defense mechanism in liver injury: insights from animal models. Food Chem. Toxicol 2013, 60:38-44.
3. Clavijo-Cornejo D., Enriquez-Cortina C., Lopez-Reyes A., Dominguez-Perez M., Nuno N., Dominguez-Meraz M., et al. Biphasic regulation of the NADPH oxidase by HGF/c-Met signaling pathway in primary mouse hepatocytes. Biochimie 2013, 95:1177-1184.
4. Daly A.K. Relevance of CYP2E1 to non-alcoholic fatty liver disease. Subcell Biochem 2013, 67:165-175.
5. Dey A., Cederbaum A.I. Induction of cytochrome P450 2E1 promotes liver injury in ob/ob mice. Hepatology 2007, 45:1355-1365.
6. Duvnjak M., Tomasic V., Gomercic M., Smircic Duvnjak L., Barsic N., Lerotic I. Therapy of nonalcoholic fatty liver disease: current status. J. Physiol. Pharmacol 2009, 60(Suppl. 7):57-66.
7. Enriquez-Cortina C., Almonte-Becerril M., Clavijo-Cornejo D., Palestino-Dominguez M., Bello-Monroy O., Nuno N., et al. Hepatocyte growth factor protects against isoniazid/rifampicin-induced oxidative liver damage. Toxicol. Sci 2013, 135:26-36.
8. French S.W., Wong K., Jui L., Albano E., Hagbjork A.L., Ingelman-Sundberg M. Effect of ethanol on cytochrome P450 2E1 (CYP2E1), lipid peroxidation, and serum protein adduct formation in relation to liver pathology pathogenesis. Exp. Mol. Pathol 1993, 58:61-75.
9. Gómez-Lechón M.J., Donato M.T., Martinez-Romero A., Jimenez N., Castell J.V., O'Connor J.E. A human hepatocellular in vitro model to investigate steatosis. Chem. Biol. Interact 2007, 165:106-116.
10. Gomez-Quiroz L.E., Paris R., Lluis J.M., Bucio L., Souza V., Hernandez E., et al. Differential modulation of interleukin 8 by interleukin 4 and interleukin 10 in HepG2 cells treated with acetaldehyde. Liver Int 2005, 25:122-130.
11. Gutierrez Ruiz M.C., Dominguez Perez M., Rodriguez Gonzalez S., Nuno Lambarri N., Licona Retama C., Gomez-Quiroz L.E. High cholesterol diet modifies the repairing effect of the hepatocyte growth factor. Gac. Med. Mex 2012, 148:236-242.
12. Han D., Hanawa N., Saberi B., Kaplowitz N. Mechanisms of liver injury. III. Role of glutathione redox status in liver injury. Am. J. Physiol. Gastrointest. Liver Physiol 2006, 291:G1-G7.
13. Lu Y., Zhuge J., Wang X., Bai J., Cederbaum A.I. Cytochrome P450 2E1 contributes to ethanol-induced fatty liver in mice. Hepatology 2008, 47:1483-1494.
14. Luo Y., Rana P., Will Y. Cyclosporine A and palmitic acid treatment synergistically induce cytotoxicity in HepG2 cells. Toxicol. Appl. Pharmacol 2012, 261:172-180.
15. Moldeus P., Hogberg J., Orrenius S. Isolation and use of liver cells. Methods Enzymol 1978, 52:60-71.
16. Negro F. Mechanisms and significance of liver steatosis in hepatitis C virus infection. World J. Gastroenterol 2006, 12:6756-6765.
17. Purohit V., Russo D., Coates P.M. Role of fatty liver, dietary fatty acid supplements, and obesity in the progression of alcoholic liver disease: introduction and summary of the symposium. Alcohol 2004, 34:3-8.
18. Reiniers M.J., van Golen R.F., van Gulik T.M., Heger M. Reactive oxygen and nitrogen species in steatotic hepatocytes: a molecular perspective on the pathophysiology of ischemia-reperfusion injury in the fatty liver. Antioxid. Redox Signal 2014, 21:1119-1142.
19. Riley T.R., Taheri M., Schreibman I.R. Does weight history affect fibrosis in the setting of chronic liver disease?. J. Gastrointestin. Liver Dis 2009, 18:299-302.
20. Robertson G., Leclercq I., Farrell G.C. Nonalcoholic steatosis and steatohepatitis. II. Cytochrome P-450 enzymes and oxidative stress. Am. J. Physiol. Gastrointest. Liver Physiol 2001, 281:G1135-G1139.
21. Rolo A.P., Teodoro J.S., Palmeira C.M. Role of oxidative stress in the pathogenesis of nonalcoholic steatohepatitis. Free Radic. Biol. Med 2012, 52:59-69.
22. Rosselli M., Lotersztajn S., Vizzutti F., Arena U., Pinzani M., Marra F. The metabolic syndrome and chronic liver disease. Curr. Pharm. Des 2014, 20:5010-5024.
23. Soardo G., Donnini D., Domenis L., Catena C., De Silvestri D., Cappello D., et al. Oxidative stress is activated by free fatty acids in cultured human hepatocytes. Metab. Syndr. Relat. Disord 2011, 9:397-401.
24. Soltys K., Dikdan G., Koneru B. Oxidative stress in fatty livers of obese Zucker rats: rapid amelioration and improved tolerance to warm ischemia with tocopherol. Hepatology 2001, 34:13-18.
25. Sumida Y., Niki E., Naito Y., Yoshikawa T. Involvement of free radicals and oxidative stress in NAFLD/NASH. Free Radic. Res 2013, 47:869-880.
26. Tashiro H., Kuroda S., Mikuriya Y., Ohdan H. Ischemia-reperfusion injury in patients with fatty liver and the clinical impact of steatotic liver on hepatic surgery. Surg. Today 2014, 44:1611-1625.
27. Tu Q.Q., Zheng R.Y., Li J., Hu L., Chang Y.X., Li L., et al. Palmitic acid induces autophagy in hepatocytes via JNK2 activation. Acta Pharmacol. Sin 2014, 35:504-512.
28. Valdes-Arzate A., Luna A., Bucio L., Licona C., Clemens D.L., Souza V., et al. Hepatocyte growth factor protects hepatocytes against oxidative injury induced by ethanol metabolism. Free Radic. Biol. Med 2009, 47:424-430.
29. Yang Y., Jiang Y., Wang Y., An W. Suppression of ABCA1 by unsaturated fatty acids leads to lipid accumulation in HepG2 cells. Biochimie 2010, 92:958-963.
30. Zakhari S. Bermuda Triangle for the liver: alcohol, obesity, and viral hepatitis. J. Gastroenterol. Hepatol 2013, 28(Suppl. 1):18-25.