Trypanosoma cruzi infectivity assessment in "in vitro" culture systems by automated cell counting

Acta Tropica

Volumn 143, Issue , 2015, Pages 47-50

  Liempi, Ana ^a   Castillo, Christian ^a   Cerda, Mauricio ^a   Droguett, Daniel ^a,d   Duaso, Juan ^a   Barahona, Katherine ^a   Hernández, Ariane ^a   Díaz Luján, Cintia ^b   Fretes, Ricardo ^b,c   Härtel, Steffen ^a   Kemmerling, Ulrike ^a  

^a UNIVERSITY OF CHILE   (Chile)

^b UNIVERSIDAD NACIONAL DE CÓRDOBA   (Argentina)

^c Universidad Nacional de LaRioja   (Argentina)

^d UNIVERSIDAD DE TALCA   (Chile)

Author keywords

Infectivity; Quantification; Trypanosoma cruzi

Indexed keywords

CHAGAS DISEASE; COMPUTER SIMULATION; CORRELATION; EPIDEMIC; NUMERICAL MODEL; PATHOLOGY; PROTOZOAN; SOFTWARE;

AMASTIGOTE; ANIMAL CELL; ARTICLE; CELL COUNTING; COMPUTER PROGRAM; CONTROLLED STUDY; IN VITRO STUDY; INTERMETHOD COMPARISON; MAMMAL CELL; NONHUMAN; PARASITE VIRULENCE; QUANTITATIVE ANALYSIS; TRYPANOSOMA CRUZI; ANIMAL; CELL COUNT; CHAGAS DISEASE; CHLOROCEBUS AETHIOPS; CULTURE TECHNIQUE; PARASITE LOAD; PARASITOLOGY; PATHOGENICITY; PROCEDURES;

LATIN AMERICA;

MAMMALIA; PROTOZOA; TRYPANOSOMA CRUZI;

ANIMALS; CELL COUNT; CELL CULTURE TECHNIQUES; CERCOPITHECUS AETHIOPS; CHAGAS DISEASE; IN VITRO TECHNIQUES; PARASITE LOAD; SOFTWARE; TRYPANOSOMA CRUZI;

EID: 84920736116     PISSN: 0001706X     EISSN: 18736254     Source Type: Journal    
DOI: 10.1016/j.actatropica.2014.12.006     Document Type: Article

References (24)

1. Amino R., Martins R.M., Procopio J., Hirata I.Y., Juliano M.A., Schenkman S. Trialysin, a novel pore-forming protein from saliva of hematophagous insects activated by limited proteolysis. J. Biol. Chem. 2002, 277(8):6207-6213.
2. Carpenter A.E., Kamentsky L., Eliceiri K.W. A call for bioimaging software usability. Nat. Methods 2012, 9(7):666-670.
3. Contreras V.T., Salles J.M., Thomas N., Morel C.M., Goldenberg S. In vitro differentiation of Trypanosoma cruzi under chemically defined conditions. Mol. Biochem. Parasitol. 1985, 16(3):315-327.
4. Coura J.R., Borges-Pereira J. Chagas disease. What is known and what should be improved: a systemic review. Rev. Soc. Bras. Med. Trop. 2012, 45(3):286-296.
5. Coura J.R., Vinas P.A. Chagas disease: a new worldwide challenge. Nature 2010, 465(7301):S6-S7.
6. De Souza W. Basic cell biology of Trypanosoma cruzi. Curr. Pharm. Des. 2002, 8(4):269-285.
7. de Souza W., de Carvalho T., Barrias E. Review on Trypanosoma cruzi: host cell interaction. Int. J. Cell Biol. 2010, 2010.
8. Diaz-Lujan C., Triquell M.F., Schijman A., Paglini P., Fretes R.E. Differential susceptibility of isolated human trophoblasts to infection by Trypanosoma cruzi. Placenta 2012, 33(4):264-270.
9. Drewlo S., Baczyk D., Dunk C., Kingdom J. Fusion assays and models for the trophoblast. Methods Mol. Biol. 2008, 475:363-382.
10. Duaso J., Rojo G., Cabrera G., Galanti N., Bosco C., Maya J., et al. Trypanosoma cruzi induces tissue disorganization and destruction of chorionic villi in an ex vivo infection model of human placenta. Placenta 2010, 31(8):705-711.
11. Faundez M., Pino L., Letelier P., Ortiz C., Lopez R., Seguel C., et al. Buthionine sulfoximine increases the toxicity of nifurtimox and benznidazole to Trypanosoma cruzi. Antimicrob. Agents Chemother. 2005, 49(1):126-130.
12. Fretes R., Kemmerling U. Mechanism of Trypanosoma cruzi placenta invasion and infection: the use of human chorionic villi explants. J. Trop. Med. 2012, 614820.
13. Gudla P.R., Nandy K., Collins J., Meaburn K.J., Misteli T., Lockett S.J. A high-throughput system for segmenting nuclei using multiscale techniques. Cytom. Part A: J. Int. Soc. Anal. Cytol. 2008, 73(5):451-466.
14. Kemmerling U., Bosco C., Galanti N. Infection and invasion mechanisms of Trypanosoma cruzi in the congenital transmission of Chagas' disease: a proposal. Biol. Res. 2010, 43(3):307-316.
15. Liempi A., Castillo C., Duaso J., Droguett D., Sandoval A., Barahona K., et al. Trypanosoma cruzi induces trophoblast differentiation: a potential local antiparasitic mechanism of the human placenta?. Placenta 2014.
16. 4-mediated effect. PLOS Neglected Trop. Dis. 2013, 7(4).
17. Nandy K., Gudla P.R., Amundsen R., Meaburn K.J., Misteli T., Lockett S.J. Supervised learning framework for screening nuclei in tissue sections. Conference proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society IEEE Engineering in Medicine and Biology Society Conference 2011, 5989-5992.
18. Toso M.A., Vial U.F., Galanti N. [Oral transmission of Chagas' disease]. Rev. Med. Chil. 2011, 139(2):258-266.
19. Triquell M.F., Diaz-Lujan C., Freilij H., Paglini P., Fretes R.E. Placental infection by two subpopulations of Trypanosoma cruzi is conditioned by differential survival of the parasite in a deleterious placental medium and not by tissue reproduction. Trans. R. Soc. Trop. Med. Hyg. 2009, 103(10):1011-1018.
20. Villalta F., Kierszenbaum F. Growth of isolated amastigotes of Trypanosoma cruzi in cell-free medium. J. Protozool. 1982, 29(4):570-576.
21. Wakamatsu M., Kikuchi A., Tamaru S., Ono K., Horikoshi T., Takagi K., et al. Voronoi diagram description of the maternal surface of the placenta: preliminary report. J. Obstet. Gynaecol. Res. 2011, 37(7):792-799.
22. Yoshida N. Molecular basis of mammalian cell invasion by Trypanosoma cruzi. An. Acad. Bras. Cienc. 2006, 78(1):87-111.
23. Yoshida N. Trypanosoma cruzi infection by oral route: how the interplay between parasite and host components modulates infectivity. Parasitol. Int. 2008, 57(2):105-109.
24. Yu W., Lee H.K., Hariharan S., Bu W., Ahmed S. Evolving generalized Voronoi diagrams for accurate cellular image segmentation. Cytom. Part A: J. Int. Soc. Anal. Cytol. 2010, 77(4):379-386.