The Kjeldahl Method as a Primary Reference Procedure for Total Protein in Certified Reference Materials Used in Clinical Chemistry. I. A Review of Kjeldahl Methods Adopted by Laboratory Medicine

Critical Reviews in Analytical Chemistry

Volumn 45, Issue 2, 2015, Pages 106-111

  Chromý, Vratislav ^a   Vinklárková, Bára ^a   Šprongl, Luděk ^b,c   Bittová, Miroslava ^a  

^a MASARYK UNIVERSITY   (Czech Republic)

^b umperk's Hospital as   (Czech Republic)

^c European Federation of Clinical Chemistry and Laboratory Medicine Working Group on Accreditation and ISO CEN standards and Working Group on Pre analytical Phase   (Czech Republic)

Author keywords

calibration; clinical chemistry; Kjeldahl methods; reference materials; total protein

Indexed keywords

BIOCHEMISTRY; BODY FLUIDS; CALIBRATION; CLINICAL LABORATORIES; NITROGEN;

CERTIFIED REFERENCE MATERIALS; CLINICAL CHEMISTRY; DIRECT ANALYSIS; KJELDAHL METHODS; LABORATORY MEDICINES; REFERENCE MATERIAL; TOTAL KJELDAHL NITROGENS; TOTAL PROTEIN;

PROTEINS;

CALIBRATION; CHEMISTRY; MEDICINE; PROTEIN; SERUM;

PROTEIN;

CALIBRATION; CHEMISTRY; CLINICAL CHEMISTRY; HUMAN; LABORATORY; MEDICINE; PROCEDURES; REFERENCE VALUE;

CALIBRATION; CHEMISTRY, CLINICAL; HUMANS; LABORATORIES; MEDICINE; PROTEINS; REFERENCE VALUES;

EID: 84920607507     PISSN: 10408347     EISSN: 15476510     Source Type: Journal    
DOI: 10.1080/10408347.2014.892820     Document Type: Review

References (41)

1. AOAC International. Official Methods of Analysis; AOAC International (formerly the Association of Official Analytical Chemists): Arlington, VA, 1995.
2. American Public Health Association, American Water Works Association, and Water Pollution Control Federation. Standard Methods for the Examination of Water and Wastewater. Definition and Procedure for the Determination of Method Detection Limits; 18th Edition. American Public Health Association: Washington, DC, 1992.
3. Archibald, R. M. Nitrogen by the Kjeldahl Method. In Standard Methods of Clinical Chemistry; Seligson, D., Ed.; Academic Press: New York, 1958; Vol. 2; p 91.
4. Beach, E. F. Nonprotein Nitrogen. In Standard Methods of Clinical Chemistry; Seligson, D., Ed.; Academic Press: New York, 1958; Vol. 2; p 100.
5. Bratstreet, R. B. Kjeldahl Method of Organic Nitrogen. Anal. Chem. 1954, 26, 185–187.
6. Cannon, D. C.; Olitzky, I.; Inkpen, J. A. Proteins. In Clinical Chemistry. Principles and Technics, 2nd ed.; Henry, R. J.; Cannon, D. C.; Winkelman, J. W., Eds.; Harper and Row: New York, 1974; p 430.
7. Chromý, V.; Sváchová, L.; Novosád, L.; Jarkovský, J.; Sedlák, P.; Horák, P.; Dobrovolná, H.; Hlávaková, B. Albumin-Based or Albumin Linked Calibrators Cause a Positive Bias in Serum Proteins Assayed by the Biuret Method. Clin. Chem. Lab. Med. 2009, 47, 91–101.
8. Doumas, B. T. Standards for Total Serum Protein Assays – A Collaborative Study. Clin. Chem. 1975, 27, 1159–1166.
9. Doumas, B. T. The Evolution of Clinical Chemistry as Reflected in Textbooks Published in the United States. Clin. Chem. 1998, 44, 2231–2233.
10. Doumas, B. T.; Bayse, D. D.; Carter, R. J.; Peters, T.; Jr., Schaffer, R. A. A Candidate Reference Method for Determination of Total Protein in Serum. I. Development and Validation. Clin. Chem. 1981a, 27, 1642–1650.
11. Doumas, B. T.; Bayse, D. D.; Borner, K.; Carter, R. J.; Elevitch, F.; Garber, C. C.; Graby, R. A.; Hause, L. L.; Mather, A.; Peters, Jr., T.; Rand, R. N.; Reeder, D. J.; Russell, S. M.; Schaffer, R.; Westgard, J. O. A Candidate Reference Method for Determination of Total Protein in Serum. II. Test for Transferability. Clin. Chem. 1981b, 27, 1651–1654.
12. Faulkner, W. R. Renal Functions Test. In Fundamentals of Clinical Chemistry; Tietz, N. W., Ed.; W.B. Saunders: Philadelphia, 1970; p 714.
13. Fraser, C. G. Biological Variation: From Principles to Practice; AACC Press: Washington, DC, 2009; p 133.
14. Grace Analytical Laboratory. Standard Operating Procedure for Total Kjeldahl Nitrogen (Lachat Method), rev. 2; Grace Analytical Laboratory: Chicago, 1994. http://www.epa.gov/glnpo/lmmb/methods/tknalr2.pdf
15. Hiller, A.; Van Slyke, D. D. A Study of Certain Protein Precipitants. J. Biol. Chem. 1922, 53, 253–267.
16. Hiller, A.; Greif, R. L.; Beckman, W. W. Determination of Protein in Urine by the Biuret Method. J. Biol. Chem. 1948a, 176, 1421–1429.
17. Hiller, A.; Plazin, J.; Van Slyke, D. D. A Study of Conditions for Kjeldahl Determination of Nitrogen in Proteins. Description of Methods with Mercury as Catalyst, Titrimetric and Gasometric Measurements of the Ammonia Formed. J. Biol. Chem. 1948b, 176, 1401–1420.
18. ISO. Laboratory Medicine – Requirements for Reference Measurement Laboratories; EN ISO 15195:2003 (85 1720); ISO: Geneva, Switzerland, 2003.
19. ISO. In Vitro Diagnostic Medical Devices – Measurement of Quantities in Samples of Biological Origin – Requirements for Content and Presentation of Reference Measurement Procedures; EN ISO 15193:2009 (85 7012); ISO: Geneva, Switzerland, 2009a.
20. ISO. In Vitro Diagnostic Medical Devices – Measurement of Quantities in Samples of Biological Origin – Requirements for Certified Reference Materials and the Content of Supporting Documentation; EN ISO 15194:2009 (85 7014); ISO: Geneva, Switzerland, 2009b.
21. ISO. Quality Management System Requirements; EN ISO 9001:2009; ISO: Geneva, Switzerland, 2009c.
22. Kachmar, J. F. Proteins and Amino Acids. In Fundamentals of Clinical Chemistry; Tietz, N. W., Ed.; W.B. Saunders: Philadelphia, 1970; p 186.
23. Kennaway, E. L. LXI. The Estimation of Non-protein Nitrogen in Blood by a Micro-Kjeldahl Method. Biochem. J. 1921, 15, 510–512.
24. Kirk, P. L. Kjeldahl Method for Total Nitrogen. Anal. Chem. 1950, 22, 354–358.
25. Kjeldahl, J. A. Neue Methode zur Bestimmung des Stickstoffs in Organischen Körpern. Z. Anal. Chem. 1883, 22, 366–382.
26. Klee, G. G.; Westgard, J. O. Quality Management. In Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 5th ed.; Burtis, C. A.; Aschwood, E. R.; Bruns, D. E., Eds.; Elsevier/Saunders: St. Louis, MO, 2012; p 163.
27. Labconco. A Guide to Kjeldahl Nitrogen Determination Methods and Apparatus. An Industry Service Publication. http://www.labconco.com
28. Ma, T. S.; Zuazaga, G. Determination of Nitrogen by Micro-Kjeldahl Procedure. Ind. Eng. Chem. Anal. Ed. 1942, 14, 280–282.
29. McKenzie, H. A.; Wallace, H. S. The Kjeldahl Determination of Nitrogen: A Critical Study of Digestion Conditions—Temperature, Catalyst, and Oxidizing Agent. Aust. J. Chem. 1954, 7, 55–70.
30. Meeker, E. W.; Wagner, E. C. Titration of Ammonia in Presence of Boric Acid. Ind. Eng. Chem. Anal. Ed. 1933, 5, 396–398.
31. Miller, L.; Houghton, J. A. The Micro-Kjeldahl Determination of the Nitrogen Content of Amino Acids and Proteins. J. Biol. Chem. 1945, 159, 373–383.
32. Möller, J. Protein Analysis Revisited. In Focus 2010, 34, 22–23.
33. Parnas, J. K.; Wagner, R. Űber die Asführung von Bestimmungen Kleine Stickstoffmengen nach Kjeldahl. Biochem. Z. 1921, 125, 253–256.
34. Persson, J. A.; Wennerholm, M.; O’Halloran, S. Handbook for Kjeldahl Digestion. A Recent Review of the Classical Method with Improvements Developed by Foss, 4th ed.; Foss: Hilleroed, Denmark, 2008.
35. Rieder, H. P. Determination of Total Protein. In Clinical Biochemistry, Principles and Methods; Curtius, H. C.; Roth, M., Eds.; Walter de Gruyter: Berlin, 1974; Vol. II; p 1468.
36. Saez-Plaza, P.; Michalowski, T.; Navas, M. J.; Asuero, A. G.; Wybraniec, S. An Overview of the Kjeldahl Method of Nitrogen Determination. Part I. Early History, Chemistry of the Procedure, and Titrimetric Finish. Crit. Rev. Anal. Chem. 2013a, 43, 178–223.
37. Saez-Plaza, P.; Navas, M. J.; Wybraniec, S.; Michalowski, T.; Asuero, A. G. An Overview of the Kjeldahl Method of Nitrogen Determination. Part II. Sample Preparation, Working Scale, Instrumental Finish and Quality Control. Crit. Rev. Anal. Chem. 2013b, 43, 224–272.
38. Van Slyke, D. D.; Hiller, A.; Dillon, R. T. Solubilities and Compositions of the Phospho-12-tungstates of the Diamino Acids and of Proline, Glycine and Tryptophane. J. Biol. Chem. 1942, 146, 137–157.
39. Vickery, H. B. The Early Years of the Kjeldahl Method to Determine Nitrogen. Yale J. Biol. Med. 1946, 18, 473–516.
40. Vinklárková, B. The Determination of Total Serum Protein by Kjeldahl Method Suitable for the Certification of Materials Used as Calibrators and Controls. Master's Diploma Thesis, Department of Chemistry, Faculty of Science, Masaryk University, Czech Republic, 2013.
41. Wagner, E. C. Titration of Ammonia in the Presence of Boric Acid in the Macro-, Semimicro- and Micro-Kjeldahl Procedures with Methyl Red Indicator and the Colour-Matching End Point. Ind. Eng. Chem. Anal. Ed. 1940, 12, 771–772.