An efficient method for decellularization of the rat liver

Journal of the Formosan Medical Association

Volumn 113, Issue 10, 2014, Pages 680-687

  Pan, Ming Xin ^a   Hu, Peng Yun ^b   Cheng, Yuan ^a   Cai, Li Quan ^a   Rao, Xiao Hui ^a   Wang, Yan ^a   Gao, Yi ^a  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

^b Xinxiang Central Hospital   (China)

Author keywords

Biocompatibility; Decellularization; Liver organ

Indexed keywords

ALBUMIN; COLLAGEN TYPE 1; COLLAGEN TYPE 3; DODECYL SULFATE SODIUM; EDETIC ACID; FIBRONECTIN; LAMININ; TRITON X 100; UREA; ALBUMINOID;

ANIMAL TISSUE; ARTICLE; BIOCOMPATIBILITY; CARDIOVASCULAR SYSTEM; CELL ADHESION; CELL INFILTRATION; COLLAGEN FIBER; CONTROLLED STUDY; EXTRACELLULAR MATRIX; FEMALE; HEPG2 CELL LINE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOGENICITY; INFLAMMATORY CELL; LIVER; LIVER DECELLULARIZATION; MALE; MUSCLE TISSUE; NONHUMAN; RAT; SCANNING ELECTRON MICROSCOPY; TISSUE ENGINEERING; TISSUE SCAFFOLD; UREA CYCLE; WESTERN BLOTTING; ANIMAL; BIOSYNTHESIS; BLOOD VESSEL PROSTHESIS; CULTURE TECHNIQUE; CYTOLOGY; MATRIX ATTACHMENT REGION; PERFUSION; PHYSIOLOGY; PROCEDURES; RABBIT; SPRAGUE DAWLEY RAT; TRANSPLANTATION;

ALBUMINS; ANIMALS; BLOOD VESSEL PROSTHESIS; CELL CULTURE TECHNIQUES; EXTRACELLULAR MATRIX; FEMALE; HEP G2 CELLS; HUMANS; LIVER; MALE; MATRIX ATTACHMENT REGIONS; PERFUSION; RABBITS; RATS; RATS, SPRAGUE-DAWLEY; TISSUE ENGINEERING; TISSUE SCAFFOLDS; UREA;

EID: 84919952458     PISSN: 09296646     EISSN: 18760821     Source Type: Journal    
DOI: 10.1016/j.jfma.2013.05.003     Document Type: Article

References (29)

1. Badylak S.F. The extracellular matrix as a scaffold for tissue reconstruction. Semin Cell Dev Biol 2002, 13:377-383.
2. Yoo J.J., Meng J., Oberpenning F., Atala A. Bladder augmentation using allogenic bladder submucosa seeded with cells. Urology 1998, 51:221-225.
3. Dahl S.L., Koh J., Prabhakar V., Niklason L.E. Decellularized native and engineered arterial scaffolds for transplantation. Cell Transplant 2003, 12:659-666.
4. Nieponice A., Gilbert T.W., Badylak S.F. Reinforcement of esophageal anastomoses with an extracellular matrix scaffold in a canine model. Ann Thorac Surg 2006, 82:2050-2058.
5. Schechner J.S., Crane S.K., Wang F., Szeglin A.M., Tellides G., Lorber M.I., et al. Engraftment of a vascularized human skin equivalent. FASEB J 2003, 17:2250-2256.
6. Macchiarini P., Jungebluth P., Go T., Asnaghi M.A., Rees L.E., Cogan T.A., et al. Clinical transplantation of a tissue-engineered airway. Lancet 2008, 372:2023-2030.
7. Ott H.C., Matthiesen T.S., Goh S.K., Black L.D., Kren S.M., Netoff T.I., et al. Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart. Nat Med 2008, 14:213-221.
8. Uygun B.E., Soto-Gutierrez A., Yagi H., Izamis M.L., Guzzardi M.A., Shulman C., et al. Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix. Nat Med 2010, 16:814-820.
9. Shupe T., Williams M., Brown A., Willenberg B., Petersen B.E. Method for the decellularization of intact rat liver. Organogenesis 2010, 6:134-136.
10. Baptista P.M., Siddiqui M.M., Lozier G., Rodriguez S.R., Atala A., Soker S. The use of whole organ decellularization for the generation of a vascularized liver organoid. Hepatology 2011, 53:604-617.
11. Barakat O., Abbasi S., Rodriguez G., Rios J., Wood R.P., Ozaki C., et al. Use of decellularized porcine liver for engineering humanized liver organ. J Surg Res 2012, 173:e11-e25.
12. Zhou P., Lessa N., Estrada D.C., Severson E.B., Lingala S., Zern M.A., et al. Decellularized liver matrix as a carrier for the transplantation of human fetal and primary hepatocytes in mice. Liver Transpl 2011, 17:418-427.
13. Bao J., Shi Y., Sun H., Yin X., Yang R., Li L., et al. Construction of a portal implantable functional tissue-engineered liver using perfusion-decellularized matrix and hepatocytes in rats. Cell Transplant 2011, 20:753-766.
14. Cartmell J.S., Dunn M.G. Effect of chemical treatments on tendon cellularity and mechanical properties. J Biomed Mater Res 2000, 49:134-140.
15. Woods T., Gratzer P.F. Effectiveness of three extraction techniques in the development of a decellularized bone-anterior cruciate ligament-bone graft. Biomaterials 2005, 26:7339-7349.
16. Aden D.P., Fogel A., Plotkin S., Damjanov I., Knowles B.B. Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. Nature 1979, 282:615-616.
17. Kang Y.Z., Wang Y., Gao Y. Decellularization technology application in the whole liver reconstruct biological scaffold. Natl Med J China 2009, 89:1135-1138.
18. Kulig K.M., Vacanti J.P. Hepatic tissue engineering. Transpl Immunol 2004, 12:303-310.
19. Svegliati-Baroni G., De Minicis S., Marzioni M. Hepatic fibrogenesis in response to chronic liver injury: novel insights on the role of cell-to-cell interaction and transition. Liver Int 2008, 28:1052-1064.
20. Bissell M.J., Aggeler J. Dynamic reciprocity: how do extracellular matrix and hormones direct gene expression?. Prog Clin Biol Res 1987, 249:251-262.
21. Mirsadraee S., Wilcox H.E., Watterson K.G., Kearney J.N., Hunt J., Fisher J., et al. Biocompatibility of acellular human pericardium. J Surg Res 2007, 143:407-414.
22. Ozeki M., Narita Y., Kagami H., Ohmiya N., Itoh A., Hirooka Y., et al. Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells. J Biomed Mater Res A 2006, 79:771-778.
23. Sellaro T.L., Ravindra A.K., Stolz D.B., Badylak S.F. Maintenance of hepatic sinusoidal endothelial cell phenotype invitro using organ-specific extracellular matrix scaffolds. Tissue Eng 2007, 13:2301-2310.
24. Lin P., Chan W.C., Badylak S.F., Bhatia S.N. Assessing porcine liver-derived biomatrix for hepatic tissue engineering. Tissue Eng 2004, 10:1046-1053.
25. Laurie G.W., Horikoshi S., Killen P.D., Segui-Real B., Yamada Y. In situ hybridization reveals temporal and spatial changes in cellular expression of mRNA for a laminin receptor, laminin, and basement membrane (type IV) collagen in the developing kidney. J Cell Biol 1989, 109:1351-1362.
26. Baldwin H.S. Early embryonic vascular development. Cardiovasc Res 1996, 31:e34-e45.
27. Rabinovitch M. Cell-extracellular matrix interactions in the ductus arteriosus and perinatal pulmonary circulation. Semin Perinatol 1996, 20:531-541.
28. Van der Rest M., Garrone R. Collagen family of proteins. FASEB J 1991, 5:2814-2823.
29. Allman A.J., McPherson T.B., Merrill L.C., Badylak S.F., Metzger D.W. The Th2-restricted immune response to xenogeneic small intestinal submucosa does not influence systemic protective immunity to viral and bacterial pathogens. Tissue Eng 2002, 8:53-62.