Antibodies for immunolabeling by light and electron microscopy: Not for the faint hearted

Histochemistry and Cell Biology

Volumn 142, Issue 4, 2014, Pages 347-360

  Griffiths, Gareth ^a   Lucocq, John Milton ^b  

^a UNIVERSITY OF OSLO   (Norway)

^b UNIVERSITY OF ST ANDREWS   (United Kingdom)

Author keywords

Antibodies Specificity; Commercial antibodies; Immunocytochemistry; Light microscopy EM; Stereology Quantitation

Indexed keywords

ANTIBODY; COMMERCIAL ANTIBODY; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY LABELING; ANTIBODY SPECIFICITY; ANTIGEN ANTIBODY COMPLEX; ELECTRON MICROSCOPY; EVALUATION STUDY; HUMAN; IMMUNOCYTOCHEMISTRY; IMMUNOGOLD LABELING; LIMIT OF QUANTITATION; MICROSCOPY; NONHUMAN; PRACTICE GUIDELINE; QUANTITATIVE HISTOCHEMISTRY; REVIEW; ANIMAL; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; PROCEDURES;

ANIMALS; ANTIBODIES; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY; MICROSCOPY, ELECTRON;

EID: 84919902892     PISSN: 09486143     EISSN: 1432119X     Source Type: Journal    
DOI: 10.1007/s00418-014-1263-5     Document Type: Review

References (45)

1. Anderson CM, Nedergaard M (2006) Emerging challenges of assigning P2X7 receptor function and immunoreactivity in neurons. Trends Neurosci 29:257–262
2. Bastos R et al (1996) Targeting and function in mRNA export of nuclear pore complex protein Nup153. J Cell Biol 134:1141–1156
3. Benicky J et al (2012) Six commercially available angiotensin II AT1 receptor antibodies are nonspecific. Cell Mol Neurobiol 32:1353–1365
4. Bodei S et al (2009) Should we be cautious on the use of commercially available antibodies to dopamine receptors? Naunyn-Schmiedeberg’s Arch Pharmacol 379:413–415
5. Bordeaux J et al (2010) Department of Pathology, Yale University School of Medicine, New Haven, CT, USA. Biotechniques 48(3):197–209
6. Burry RW (2011) Controls for immunocytochemistry an update. J Histochem Cytochem 59:6–12
7. Couchman JR (2009) Commercial antibodies: the good, bad, and really ugly. J Histochem Cytochem 57:7–8
8. Dalcik H, Dalcik C (2012) Trends in immunolabeled and Related techniques. In: Abuelzein E (ed) Biochemistry, genetics and molecular biology. InTechOpen. ISBN 978-953-51-0570-1
9. Everaerts W et al (2009) Where is TRPV1 expressed in the bladder, do we see the real channel? Naunyn-Schmiedeberg’s Arch Pharmacol 379:421–425
10. Frank SA (2002) Immunology and evolution of infectious disease. Princeton University Press, Princeton
11. Fritschy JM (2008) Is my antibody-staining specific? How to deal with pitfalls of immunohistochemistry. Eur J Neurosci 28:2365–2370
12. Griffiths G et al (1993) Fine structure immunocytochemistry. Springer, Heidelberg
13. Hacker C, Lucocq JM (2014) Analysis of specificity in immunoelectron microscopy. Methods Mol Biol 1117:315–323
14. Harlow E et al (1988) Laboratory manual. Cold Spring Harbor Laboratory, New York, pp 310–311
15. Holmseth S et al (2005) Specificity of antibodies: unexpected cross-reactivity of antibodies directed against the excitatory amino acid transporter 3 (EAAT3). Neuroscience 136:649–660
16. Holmseth S et al (2006) Specificity controls for immunocytochemistry. Anat Embryol 211:257–266
17. Holmseth S et al (2012) Specificity controls for immunocytochemistry the antigen preadsorption test can lead to inaccurate assessment of antibody specificity. J Histochem Cytochem 60:174–187
18. James LC, Tawfik DS (2003) The specificity of cross-reactivity: promiscuous antibody binding involves specific hydrogen bonds rather than nonspecific hydrophobic stickiness. Protein Sci 12:2183–2193
19. Jensen BC et al (2009) Ten commercial antibodies for alpha-1-adrenergic receptor subtypes are nonspecific. Naunyn Schmiedebergs Arch Pharmacol 379:409–412
20. Josephsen K et al (1999) Selective but nonspecific immunolabeling of enamel protein-associated compartments by a monoclonal antibody against vimentin. J Histochem Cytochem 47:1237–1245
21. Jositsch G et al (2009) Suitability of muscarinic acetylcholine receptor antibodies for immunohistochemistry evaluated on tissue sections of receptor gene-deficient mice. Naunyn-Schmiedeberg’s Arch Pharmacol 379:389–395
22. Kalyuzhny AE (2009) The dark side of the immunohistochemical moon: industry. J Histochem Cytochem 57:1099–1101
23. Kirkpatrick P (2009) Technology: specificity concerns with antibodies for receptor mapping. Nat Rev Drug Discov 8:278
24. Lescar J et al (1995) Crystal structure of a cross-reaction complex between Fab F9. 13.7 and guinea fowl lysozyme. J Biol Chem 270:18067–18076
25. Lorincz A, Nusser Z (2008) Specificity of immunoreactions: the importance of testing specificity in each method. J Neurosci 28:9083–9086
26. Lu X, Bartfai T (2009) Analyzing the validity of GalR1 and GalR2 antibodies using knockout mice. Naunyn-Schmiedeberg’s Arch Pharmacol 379:417–420
27. Lucocq J (2008) Quantification of structures and gold labeling in transmission electron microscopy. Methods Cell Biol 88:59
28. Lucocq J (2012) Can data provenance go the full monty? Trends Cell Biol 22:229–230
29. Lucocq JM, Gawden-Bone C (2010) Quantitative assessment of specificity in immunoelectron microscopy. J Histochem Cytochem 58:917–927
30. Lucocq JM et al (2004) A rapid method for assessing the distribution of gold labeling on thin sections. J Histochem Cytochem 52:991–1000
31. Nigg E et al (1982) Immunofluorescent localization of the transforming protein of Rous sarcoma virus with antibodies against a synthetic src peptide. Proc Natl Acad Sci 79:5322–5326
32. Petrusz P et al (1976) Specificity in immunocytochemical staining. J Histochem Cytochem 24:1110–1112
33. Pradidarcheep W et al (2009) Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors. Naunyn-Schmiedeberg’s Arch Pharmacol 379:397–402
34. Ramos-Vara JA (2005) Technical aspects of immunohistochemistry. Vet Pathol 42:405–426
35. Rhodes KJ, Trimmer JS (2006) Antibodies as valuable neuroscience research tools versus reagents of mass distraction. J Neurosci 26:8017–8020
36. Saper CB (2005) An open letter to our readers on the use of antibodies. J Comp Neurol 493:477–478
37. Saper CB (2009) A guide to the perplexed on the specificity of antibodies. J Histochem Cytochem 57:1–5
38. Saper CB, Sawchenko PE (2003) Magic peptides, magic antibodies: guidelines for appropriate controls for immunohistochemistry. J Comp Neurol 465:161–163
39. Schwarz H, Humbel BM (2007) Correlative light and electron microscopy using immunolabeled resin sections. In: Kuo J (ed) Electron microscopy. Humana press, Totowa, NJ, pp 229–25
40. Soderqvist H et al (1996) Formation of nuclear bodies in cells overexpressing the nuclear pore protein POM121. Exp Cell Res 225:75–84
41. Swaab DF et al (1977) Can specificity ever be proved in immunocytochemical staining. J Histochem Cytochem 25:388–390
42. Thiede B, Koehler CJ, Strozynski M, Treumann A, Stein R, Zimny-Arndt U, Schmid M, Jungblut PR (2013) High resolution quantitative proteomics of HeLa cells protein species using stable isotope labeling with amino acids in cell culture (SILAC), two-dimensional gel electrophoresis (2DE) and nano-liquid chromatography coupled to an LTQ-OrbitrapMass spectrometer. Mol Cell Proteomics 12:529
43. Varga JM et al (1991) Mechanism of allergic cross-reactions—I. Multispecific binding of ligands to a mouse monoclonal anti-DNP IgE antibody. Mol Immunol 28:641–654
44. Watanabe M et al (1998) Selective scarcity of NMDA receptor channel subunits in the stratum lucidum (mossy fibre-recipient layer) of the mouse hippocampal CA3 subfield. Eur J Neurosci 10:478–487
45. Yu W, Hill WG (2013) Lack of specificity shown by P2Y6 receptor antibodies. Naunyn-Schmiedeberg’s Arch Pharmacol 386:885–891