Electrophoretic mobility shift assay of RNA-RNA complexes

Methods in Molecular Biology

Volumn 1240, Issue , 2015, Pages 153-163

  Bak, Geunu ^a   Han, Kook ^a   Kim, Kwang Sun ^b   Lee, Younghoon ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Electrophoretic mobility shift assay (EMSA); RNA RNA complex; RNA RNA interaction

Indexed keywords

RNA; MESSENGER RNA; RNA POLYMERASE II;

ARTICLE; COMPLEX FORMATION; DEPHOSPHORYLATION; GEL MOBILITY SHIFT ASSAY; IN VITRO STUDY; INTERACTIONS WITH RNA; ISOTOPE LABELING; RNA BINDING; RNA PURIFICATION; RNA SYNTHESIS; RNA TRANSCRIPTION; GENETIC TRANSCRIPTION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MUTATION; PROCEDURES; SACCHAROMYCES CEREVISIAE; STAINING;

ELECTROPHORETIC MOBILITY SHIFT ASSAY; MUTATION; RNA; RNA POLYMERASE II; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; STAINING AND LABELING; TRANSCRIPTION, GENETIC;

EID: 84919682870     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1896-6_12     Document Type: Article

References (15)

1. Hendrickson W, Schleif RF (1984) Regulation of the Escherichia coli L-arabinose operon studied by gel electrophoresis DNA binding assay. J Mol Biol 178:611-628
2. Revzin A (1989) Gel electrophoresis assays for DNA-protein interactions. Biotechniques 7:346-355
3. Riggs AD, Suzuki H, Bourgeois S (1970) Lac repressor-operator interaction. I. Equilibrium studies. J Mol Biol 48:67-83
4. Fried M, Crothers DM (1981) Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res 9:6505-6525
5. Chodosh LA, Carthew RW, Sharp PA (1986) A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor. Mol Cell Biol 6:4723-4733
6. Fried MG (1989) Measurement of protein- DNA interaction parameters by electrophoresis mobility shift assay. Electrophoresis 10: 366-376
7. Fried MG, Crothers DM (1984) Kinetics and mechanism in the reaction of gene regulatory proteins with DNA. J Mol Biol 172: 263-282
8. Ko J, Lee Y, Park I, Cho B (2001) Identification of a structural motif of 23S rRNA interacting with 5S rRNA. FEBS Lett 508:300-304
9. Han K, Kim KS, Bak G, Park H, Lee Y (2010) Recognition and discrimination of target mRNAs by Sib RNAs, a cis-encoded sRNA family. Nucleic Acids Res 38:5851-5866
10. Price SR, Ito N, Oubridge C, Avis JM, Nagai K (1995) Crystallization of RNA-protein complexes. I. Methods for the large-scale preparation of RNA suitable for crystallographic studies. J Mol Biol 249:398-408
11. Tzakos AG, Easton LE, Lukavsky PJ (2007) Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies. Nat Protoc 2:2139-2147
12. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
13. Gesteland RF, Cech T, Atkins JF (2006) The RNA world: the nature of modern RNA suggests a prebiotic RNA world, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
14. Garner MM, Revzin A (1981) A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system. Nucleic Acids Res 9: 3047-3060
15. Persson C, Wagner EG, Nordstrom K (1988) Control of replication of plasmid R1: kinetics of in vitro interaction between the antisense RNA, CopA, and its target, CopT. EMBO J 7: 3279-3288