Derivation of ipscs after culture of human dental pulp cells under defined conditions

PLoS ONE

Volumn 9, Issue 12, 2014, Pages

  Takeda Kawaguchi, Tomoko ^a   Sugiyama, Ken ^a   Chikusa, Shunji ^a   Iida, Kazuki ^a   Aoki, Hitomi ^a   Tamaoki, Naritaka ^a   Hatakeyama, Daijiro ^a   Kunisada, Takahiro ^a   Shibata, Toshiyuki ^a   Fusaki, Noemi ^b   Tezuka, Ken Ichi ^a  

^a GIFU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; KRUPPEL LIKE FACTOR 4; OCTAMER TRANSCRIPTION FACTOR 4; OSTEOPONTIN; STAGE SPECIFIC EMBRYO ANTIGEN 4; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR RUNX2; TRANSCRIPTION FACTOR SOX2;

ADOLESCENT; ARTICLE; BGLAP GENE; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL ISOLATION; CELL STRUCTURE; CHROMOSOME NUMBER; CHROMOSOME STRUCTURE; COLONY FORMATION; DNA MICROARRAY; ENZYME ACTIVITY; FEMALE; GENE; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; KLF4 GENE; MOUSE; NANOG GENE; NONHUMAN; OCT3 4 GENE; ONCOGENE C MYC; PLURIPOTENT STEM CELL; REAL TIME POLYMERASE CHAIN REACTION; REX1 GENE; RUNX2 GENE; SENDAI VIRUS; SOX2 GENE; SPP1 GENE; STEM CELL TRANSPLANTATION; TOOTH PULP; UPREGULATION; CYTOLOGY; PRIMARY CELL CULTURE; PROCEDURES;

ANIMALIA; BOVINAE;

ADOLESCENT; CELL DIFFERENTIATION; CELLS, CULTURED; DENTAL PULP; HUMANS; INDUCED PLURIPOTENT STEM CELLS; PRIMARY CELL CULTURE;

EID: 84919363659     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0115392     Document Type: Article

References (27)

1. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, et al. (2002) Stem cell properties of human dental pulp stem cells. J Dent Res 81: 531-535.
2. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S (2000) Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA 97: 13625-13630.
3. Takeda T, Tezuka Y, Horiuchi M, Hosono K, Iida K, et al. (2008) Characterization of dental pulp stem cells of human tooth germs. J Dent Res 87: 676-681.
4. Iida K, Takeda-Kawaguchi T, Tezuka Y, Kunisada T, Shibata T, et al. (2010) Hypoxia enhances colony formation and proliferation but inhibits differentiation of human dental pulp cells. Arch Oral Biol 55: 648-654.
5. Erickson GA, Bolin SR, Landgraf JG (1991) Viral contamination of fetal bovine serum used for tissue culture: risks and concerns. Dev Biol Stand 75: 173-175.
6. Eloit M (1999) Risks of virus transmission associated with animal sera or substitutes and methods of control. Dev Biol Stand 99: 9-16.
7. Shah G (1999) Why do we still use serum in the production of biopharmaceuticals? Dev Biol Stand 99: 17-22.
8. Wessman SJ, Levings RL (1999) Benefits and risks due to animal serum used in cell culture production. Dev Biol Stand 99: 3-8.
9. Willkommen H, Scheiblauer H, Lower J (1999) Serum and serum substitutes: virus safety by inactivation or removal. Dev Biol Stand 99: 131-138.
10. Van der Valk J, Mellor D, Brands R, Fischer R, Gruber F, et al. (2004) The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxic in Vitro 18: 1-12.
11. Knight R (2010) The risk of transmitting prion disease by blood or plasma products. Transfus Apher Sci 43: 387-391.
12. Ferro F, Spelat R, Beltrami AP, Cesselli D, Curcio F (2012) Isolation and characterization of human dental pulp derived stem cells by using media containing low human serum percentage as clinical grade substitutes for bovine serum. PLoS One. 7: e41774.
13. Escobedo-Lucea C, Bellver C, Gandia C, Sanz-Garcia A, Esteban FJ, et al. (2013) A xenogeneic-free protocol for isolation and expansion of human adipose stem cells for clinical uses. PLoS One. 8: e67870.
14. Tamaoki N, Takahashi K, Tanaka T, Ichisaka T, Aoki H, et al. (2010) Dental pulp cells for induced pluripotent stem cell banking. J Dent Res 89: 773-778.
15. Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, et al. (2011) A more efficient method to generate integration-free human iPS cells. Nat Methods 8: 409-412.
16. Fusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M (2009) Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome. Proc Jpn Acad Ser B Phys Biol Sci. 85: 348-362.
17. Ban H, Nishishita N, Fusaki N, Tabata T, Saeki T, et al. (2011) Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci USA 108 (34): 14234-14239.
18. Iida K, Takeda-Kawaguchi T, Hada M, Yuriguchi M, Aoki H, et al. (2013) Hypoxia-enhanced derivation of iPSCs from human dental pulp cells. J Dent Res 92: 905-910.
19. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
20. Watanabe K, Ueno M, Kamiya D, Nishiyama A, Matsumura M, et al. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-686.
21. Horwitz EM, Prockop DJ, Fitzpatrick LA, Koo WW, Gordon PL, et al. (1999) Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta. Nat Med 5: 309-313.
22. Spees JL, Gregory CA, Singh H, Tucker HA, Peister A, et al. (2004) Internalized antigens must be removed to prepare hypoimmunogenic mesenchymal stem cells for cell and gene therapy. Mol Ther 9: 747-756.
23. Yan X, Qin H, Qu C, Tuan RS, Shi S, et al. (2010) iPS cell reprogrammed from human mesenchymal-like stem/progenitor cells of dental tissue origin. Stem Cells Dev 19: 469-480.
24. Beltrão-Braga PC, Pignatari GC, Maiorka PC, Oliveira NA, Lizier NF, et al. (2011) Feeder-free derivation of induced pluripotent stem cells from human immature dental pulp stem cells. Cell Transplant 20: 1707-1719.
25. Oda Y, Yoshimura Y, Ohnishi H, Tadokoro M, Katsube Y, et al. (2010) Induction of pluripotent stem cells from human third molar mesenchymal stromal cells. J Biol Chem 17: 29270-29278.
26. Lizier NF, Kerkis I, Wenceslau CV (2013) Generation of Induced Pluripotent Stem Cells from Dental Pulp Somatic Cells. In: Bhartiya D, editor. INTECH: 131-149.
27. The International Stem Cell Initiative (2007) Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nat Biotechnol 25: 803-816.