A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples

Diagnostic Microbiology and Infectious Disease

Volumn 80, Issue 4, 2014, Pages 285-286

  Sedlak, Ruth Hall ^a   Kuypers, Jane ^a   Jerome, Keith R ^a,b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b Fred Hutchinson Cancer Research Center   (United States)

Author keywords

Digital PCR; Inhibition; Quantitative PCR; Stool

Indexed keywords

ARTICLE; CONTROLLED STUDY; FECES ANALYSIS; HUMAN; HUMAN ADENOVIRUS; HUMAN ADENOVIRUS SPECIES F; HUMAN CYTOMEGALOVIRUS; INTERMETHOD COMPARISON; MULTIPLEX POLYMERASE CHAIN REACTION; MULTIPLEXED DROPLET DIGITAL PCR ASSAY; NONHUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE PCR ASSAY; SENSITIVITY ANALYSIS; VIRUS DETECTION; COMPARATIVE STUDY; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; FECES; GENETICS; HUMAN ADENOVIRUS C; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; VIROLOGY;

ADENOVIRUSES, HUMAN; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; FECES; HUMANS; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84919339207     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2014.09.004     Document Type: Article

References (9)

1. Alaeddini R. Forensic implications of PCR inhibition-a review. Forensic Sci Int Genet 2012, 6:297-305.
2. Boeckh M., Huang M., Ferrenberg J., Stevens-Ayers T., Stensland L., Nichols W.G., et al. Optimization of quantitative detection of cytomegalovirus DNA in plasma by real-time PCR. J Clin Microbiol 2004, 42:1142-1148.
3. Dingle T.C., Sedlak R.H., Cook L., Jerome K.R. Tolerance of droplet-digital PCR vs real-time quantitative PCR to inhibitory substances. Clin Chem 2013, 59:1670-1672.
4. Hall Sedlak R., Jerome K.R. The potential advantages of digital PCR for clinical virology diagnostics. Expert Rev Mol Diagn 2014, 14:501-507.
5. Haynes R.J., Kline M.C., Toman B., Scott C., Wallace P., Butler J.M., et al. Standard reference material 2366 for measurement of human cytomegalovirus DNA. J Mol Diagn 2013, 15:177-185.
6. Herfarth H.H., Long M.D., Rubinas T.C., Sandridge M., Miller M.B. Evaluation of a non-invasive method to detect cytomegalovirus (CMV)-DNA in stool samples of patients with inflammatory bowel disease (IBD): a pilot study. Dig Dis Sci 2010, 55:1053-1058.
7. Huang M.L., Nguy L., Ferrenberg J., Boeckh M., Cent A., Corey L. Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses. Diagn Microbiol Infect Dis 2008, 62:263-271.
8. Schrader C., Schielke A., Ellerbroek L., Johne R. PCR inhibitors-occurrence, properties and removal. J Appl Microbiol 2012, 113:1014-1026.
9. Sedlak R.H., Cook L., Huang M.L., Magaret A., Zerr D.M., Boeckh M., et al. Identification of chromosomally integrated human herpesvirus 6 by droplet digital PCR. Clin Chem 2014, 60:765-772.