Use of a high-throughput in vitro microfluidic system to develop oral multi-species biofilms

Journal of Visualized Experiments

Volumn , Issue 94, 2014, Pages

  Samarian, Derek S ^a   Jakubovics, Nicholas S ^b   Luo, Ting L ^a   Rickard, Alexander H ^a  

^a UNIVERSITY OF MICHIGAN SCHOOL OF PUBLIC HEALTH   (United States)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

Bioengineering; Biofilm; COMSTAT; Confocal laser scanning microscopy; Dental plaque; Image analysis; Image reconstruction; IMAGEJ; IMARIS; Issue 94; Modeling; Multi species biofilm communities; Pyrosequencing; Saliva; Three dimensional structure

Indexed keywords

EID: 84917695114     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52467     Document Type: Article

References (42)

1. Stoodley, P., Sauer, K., Davies, D. G., Costerton, J. W. Biofilms as complex differentiated communities. Annual review of microbiology. 56, 187-209 (2002).
2. Wimpenny, J. Microbial metropolis. Advances in microbial physiology. 56, 29-84 (2009).
3. Jakubovics, N. S., Kolenbrander, P. E. The road to ruin: the formation of disease-associated oral biofilms. Oral diseases. 16, (8), 729-739 (2010).
4. Mah, T. F., O'Toole, G. A. Mechanisms of biofilm resistance to antimicrobial agents. Trends in microbiology. 9, (1), 34-39 (2001).
5. Cate, J. M., Zaura, E. The numerous microbial species in oral biofilms: how could antibacterial therapy be effective. Advances in dental research. 24, (2), 108-111 (2012).
6. Gilbert, P., Maira-Litran, T., McBain, A. J., Rickard, A. H., Whyte, F. W. The physiology and collective recalcitrance of microbial biofilm communities. Advances in microbial physiology. 46, 202-256 (2002).
7. Anon, Centers for Disease Control and Prevention (CDC): Oral health: Preventing cavities, gum disease, tooth loss, and oral cancers. http://www. cdc. gov/chronicdisease/resources/publications/AAG/doh. htm#aag(2011).
8. Smith, H. Microbial behavior, 'in vivo' and 'in vitro. Smith, H., Taylor, J. 1-29 Cambridge University Press (1964).
9. Haffajee, A. D., Socransky, S. S. Introduction to microbial aspects of periodontal biofilm communities, development and treatment. Periodontology. 42, 7-12 (2000).
10. McBain, A. J. Chapter 4: In vitro biofilm models: an overview. Advances in applied microbiology. 69, 99-132 (2009).
11. Baehni, P. C., Takeuchi, Y. Anti-plaque agents in the prevention of biofilm-associated oral diseases. Oral diseases. 9 Suppl 1, 23-29 (2003).
12. Graves, D. T., Kang, J., Andriankaja, O., Wada, K., Rossa, C. Animal models to study host-bacteria interactions involved in periodontitis. Frontiers of oral biology. 15, 117-132 (2012).
13. Chun, J., Kim, K. Y., Lee, J. H., Choi, Y. The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer. BMC microbiology. 10, 101(2010).
14. Diaz, P. I., et al. Molecular characterization of subject-specific oral microflora during initial colonization of enamel. Applied and environmental microbiology. 72, (4), 2837-2848 (2006).
15. Auschill, T. M., et al. Effect of two antimicrobial agents on early in situ biofilm formation. Journal of clinical periodontology. 32, (2), 147-152 (2005).
16. Coenye, T., Nelis, H. J. In vitro and in vivo model systems to study microbial biofilm formation. Journal of microbiological. 83, (2), 89-105 (2010).
17. Donlan, R. M. Role of biofilms in antimicrobial resistance. ASAIO journal. 46, (6), 47-52 (2000).
18. Wimpenny, J. W. The validity of models. Advances in dental research. 11, (1), 150-159 (1997).
19. Umland, T. C., et al. In vivo-validated essential genes identified in Acinetobacter baumannii by using human ascites overlap poorly with essential genes detected on laboratory media. 3, (4), (2012).
20. Nance, W. C., et al. A high-throughput microfluidic dental plaque biofilm system to visualize and quantify the effect of antimicrobials. The Journal of antimicrobial chemotherapy. 68, (11), 2550-2560 (2013).
21. Werner, E., et al. Stratified growth in Pseudomonas aeruginosa biofilms. Applied and environmental microbiology. 70, (10), 6188-6196 (2004).
22. Rueden, C. T., Eliceiri, K. W. Visualization approaches for multidimensional biological image data. BioTechniques. 43, (1 Suppl), 33-36 (2007).
23. Collins, T. J. ImageJ for microscopy. BioTechniques. 43, 25-30 (2007).
24. Rao, D., Arvanitidou, E., Du-Thumm, L., Rickard, A. H. Efficacy of an alcohol-free CPC-containing mouthwash against oral multispecies biofilms. The Journal of clinical dentistry. 22, (6), 187-194 (2011).
25. Rusconi, R., Garren, M., Stocker, R. Microfluidics expanding the frontiers of microbial ecology. Annual review of biophysics. 43, 65-91 (2014).
26. Kim, J., Park, H. D., Chung, S. Microfluidic approaches to bacterial biofilm formation. Molecules. 17, (8), 9818-9834 (2012).
27. Mosier, A. P., Cady, N. C. Analysis of bacterial surface interactions using microfluidic systems. Science progress. 94, (4), 431-450 (2011).
28. Foster, J. S., Kolenbrander, P. E. Development of a multispecies oral bacterial community in a saliva-conditioned flow cell. Applied and environmental microbiology. 70, (7), 4340-4348 (2004).
29. Cuadra-Saenz, G., et al. Autoinducer-2 influences interactions amongst pioneer colonizing streptococci in oral biofilms. Microbiology. 158, (7), 1783-1795 (2012).
30. Rickard, A. H., et al. Autoinducer 2: a concentration-dependent signal for mutualistic bacterial biofilm growth). Molecular microbiology. 60, (6), 1446-1456 (2006).
31. Corbin, A., Pitts, B., Parker, A., Stewart, P. S. Antimicrobial penetration and efficacy in an in vitro oral biofilm model. Antimicrobial agents and chemotherapy. 55, (7), 3338-3344 (2011).
32. Diggle, M. A., Clarke, S. C. Pyrosequencing: sequence typing at the speed of light. Molecular biotechnology. 28, (2), 129-137 (2004).
33. Hiyari, S., Bennett, K. M. Dental diagnostics: molecular analysis of oral biofilms. Journal of dental hygiene: JDH/American Dental Hygienists' Association. 85, (4), 256-263 (2011).
34. Filoche, S., Wong, L., Sissons, C. H. Oral biofilms: emerging concepts in microbial ecology. Journal of dental research. 89, (1), 8-18 (2010).
35. Xu, J. Microbial ecology in the age of genomics and metagenomics: concepts, tools, and recent advances. Molecular ecology. 15, (7), 1713-1731 (2006).
36. Rogers, G. B., Carroll, M. P., Bruce, K. D. Studying bacterial infections through culture-independent approaches. Journal of medical microbiology. 58, (11), 1401-1418 (2009).
37. Socransky, S. S., et al. The microbiota of the gingival crevice area of man. I. Total microscopic and viable counts and counts of specific organisms. Archives of oral biology. 8, 275-280 (1963).
38. Heydorn, A., et al. Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology. 146, (10), 2395-2407 (2000).
39. Nobbs, A. H., Lamont, R. J., Jenkinson, H. F. Streptococcus adherence and colonization. Microbiology and molecular biology reviews). MMBR. 73, (3), 407-450 (2009).
40. Hope, C. K., Clements, D., Wilson, M. Determining the spatial distribution of viable and nonviable bacteria in hydrated microcosm dental plaques by viability profiling. Journal of applied microbiology. 93, (3), 448-455 (2002).
41. Adams, H., et al. Development of a laboratory model to assess the removal of biofilm from interproximal spaces by powered tooth brushing. American journal of dentistry Spec No 12B-17B. 15, 12-17 (2002).
42. Ledder, R. G., McBain, A. J. An in vitro comparison of dentifrice formulations in three distinct oral microbiotas. Archives of oral biology. 57, (2), 139-147 (2012).