Overexpression of the skNAC gene in human rhabdomyosarcoma cells enhances their differentiation potential and inhibits tumor cell growth and spreading

Clinical and Experimental Metastasis

Volumn 31, Issue 8, 2014, Pages 869-879

  Berkholz, Janine ^a   Kuzyniak, Weronika ^a   Hoepfner, Michael ^a   Munz, Barbara ^b  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

Author keywords

Metastasis; Myogenic differentiation; Rhabdomyosarcoma; skNAC

Indexed keywords

MYOGENIN; MYOSIN HEAVY CHAIN; CHAPERONE; MESSENGER RNA; NASCENT-POLYPEPTIDE-ASSOCIATED COMPLEX;

ARTICLE; CANCER INHIBITION; CELL CYCLE PROGRESSION; CELL DIFFERENTIATION; CONTROLLED STUDY; GENE; GENE EXPRESSION; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; METASTASIS; MULTINUCLEAR CELL; MYOBLAST; MYOTUBE; PROCEDURES; RHABDOMYOSARCOMA CELL LINE; SKNAC GENE; SOFT AGAR ASSAY; TUMOR GROWTH; APOPTOSIS; CELL PROLIFERATION; ENZYME IMMUNOASSAY; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; METABOLISM; NORTHERN BLOTTING; PATHOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHABDOMYOSARCOMA; WESTERN BLOTTING;

APOPTOSIS; BLOTTING, NORTHERN; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELL PROLIFERATION; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION; HUMANS; IMMUNOENZYME TECHNIQUES; MOLECULAR CHAPERONES; MYOBLASTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHABDOMYOSARCOMA; RNA, MESSENGER;

EID: 84916931845     PISSN: 02620898     EISSN: 15737276     Source Type: Journal    
DOI: 10.1007/s10585-014-9676-z     Document Type: Article

References (30)

1. Xia SJ, Pressey JG, Barr FG (2002) Molecular pathogenesis of rhabdomyosarcoma. Cancer Biol Ther 1:97–104
2. De Giovanni C, Landuzzi L, Nicoletti G, Lollini P-L, Nanni P (2009) Molecular and cellular biology of rhabdomyosarcoma. Fut Oncol 5:1449–1475
3. Breitfeld PP, Meyer WH (2005) Rhabdomyosarcoma: new windows of opportunity. Oncologist 10:518–527
4. Lollini P-L, De Giovanni C, Landuzzi L, Nicoletti G, Scotlandi K, Nanni P (1991) Reduced metastatic ability of in vitro differentiated human rhabdomyosarcoma cells. Inv Metast 11:116–124
5. Wiedmann B, Sakai H, Davis TA, Wiedmann M (1994) A protein complex required for signal-sequence-specific sorting and translocation. Nature 370:434–440
6. Preissler S, Deuerling E (2012) Ribosome-associated chaperones as key players in proteostasis. Trends Biochem Sci 37:74–83
7. Yotov WV, St-Arnaud R (1996) Differential splicing-in of a proline-rich exon converts alpha NAC into a muscle-specific transcription factor. Genes Dev 10:1763–1772
8. Otten A, Firpo E, Gerber A, Brody L, Roberts J, Tapscott S (1997) Inactivation of MyoD-mediated expression of p21 in tumor cell lines. Cell Growth Diff 8:1151–1160
9. Astolfi A, De Giovanni C, Landuzzi L, Nicoletti G, Ricci C, Croci S, Scopece L, Nanni P, Lollini P-L (2001) Identification of new genes related to the myogenic differentiation arrest of human rhabdomyosarcoma cells. Gene 274:139–149
10. Nanni P, Nicoletti G, Palladini A, Astolfi A, Rinella P, Croci S, Landuzzi L, Monduzzi G, Stivani V, Antognoli A, Murgo A, Ianzano M, De Giovanni C, Lollini PL (2009) Opposing control of rhabdomyosarcoma growth and differentiation by myogenin and interleukin 4. Mol Cancer Ther 8:754–761
11. Aguanno S, Bouchè M, Adamo S, Molinaro M (1990) 12-O-tetradecanoylphorbol-13-acetate-induced differentiation of a human rhabdomyosarcoma cell line. Cancer Res 50:3377–3382
12. Avirneni-Vadlamudi U, Galindo KA, Endicott TR, Paulson V, Cameron S, Galindo RL (2012) Drosophila and mammalian models uncover a role for the myoblast fusion gene TANC1 in rhabdomyosarcoma. J Clin Invest 122:403–407
13. De Giovanni C, Nanni P, Nicoletti G, Ceccarelli C, Scotlandi K, Landuzzi L, Lollini P-L (1989) Metastatic ability and differentiative properties of a new cell line of human embryonal rhabdomyosarcoma (CCA). Anticancer Res 9:1943–1949
14. Douglass EC, Valentine M, Etcubanas E, Parham D, Webber BL, Houghton PJ, Houghton JA, Green AA (1987) A specific chromosomal abnormality in rhabdomyosarcoma. Cytogenet Cell Genet 45:148–155
15. Thulasi R, Dias P, Houghton PJ, Houghton JA (1996) α2a-Interferon-induced differentiation of human alveolar rhabdomyosarcoma cells: correlation with down-regulation of the insulin-like growth factor type I receptor. Cell Growth Diff 7:531–541
16. Miekus K, Lukasiewicz E, Jarocha D, Sekula M, Drabik G, Majka M (2013) The decreased metastatic potential of rhabdomyosarcoma cells obtained through MET receptor downregulation and the induction of differentiation. Cell Death Dis 4:e459. doi:10.1038/cdds.2012.199
17. Rees H, Williamson D, Papanastasiou A, Jina N, Nabarro S, Shipley J, Anderson J (2006) The MET receptor tyrosine kinase contributes to invasive tumor growth in rhabdomyosarcomas. Growth Factors 24:197–208
18. Rapa E, Hill SK, Morten KJ, Potter M, Mitchell C (2012) The over-expression of cell migratory genes in alveolar rhabdomyosarcoma could contribute to metastatic spread. Clin Exp Metast 29:419–429
19. Munz B, Hildt E, Springer ML, Blau HM (2002) RIP2, a checkpoint in myogenic differentiation. Mol Cell Biol 22:5879–5886
20. Munz B, Wiedmann M, Lochmüller H, Werner S (1999) Cloning of novel injury-regulated genes. Implications for an important role of the muscle-specific protein skNAC in muscle repair. J Biol Chem 274:13305–13310
21. Gloesenkamp C, Nitzsche B, Lim AR, Normant E, Vosburgh E, Schrader M, Ocker M, Scherübl M, Höpfner M (2012) Heat shock protein 90 is a promising target for effective growth inhibition of gastrointestinal neuroendocrine tumors. Int J Oncol 40:1659–1667
22. Berger F, Berkholz J, Breustedt T, Ploen D, Munz B (2012) Skeletal muscle-specific variant of nascent polypeptide associated complex alpha (skNAC): implications for a specific role in mammalian myoblast differentiation. Eur J Cell Biol 91:150–155
23. Annavarapu SR, Cialfi S, Dominici C, Kokai GK, Uccini S, Ceccarelli S, McDowell HP, Helliwell TR (2013) Characterization of Wnt/β-catenin signaling in rhabdomyosarcoma. Lab Invest 93:1090–1099
24. Berkholz J, Zakrzewicz A, Munz B (2013) skNAC depletion stimulates myoblast migration and perturbs sarcomerogenesis by enhancing calpain 1 and 3 activity. Biochem J 453:303–310
25. Fulda S (2008) Targeting apoptosis resistance in rhabdomyosarcoma. Curr Cancer Drug Targets 8:536–544
26. Li P, Zhao Y, Wu X, Xia M, Fang M, Iwasaki Y, Sha J, Chen Q, Xu Y, Shen A (2012) Interferon gamma (IFN-γ) disrupts energy expenditure and metabolic homeostasis by suppressing SIRT1 transcription. Nucl Acids Res 40:1609–1620
27. Londhe P, Davie J (2011) Gamma interferon modulates myogenesis through the major histocompatibility complex class II transactivator, CIITA. Mol Cell Biol 31:2854–2866
28. Londhe P, Zhu B, Abraham J, Keller C, Davie J (2012) CIITA is silenced by epigenetic mechanisms that prevent the recruitment of transactivating factors in rhabdomyosarcoma cells. Int J Cancer 131(4):E437–E448. doi:10.1002/ijc.26478
29. Kim JR, Moon YJ, Kwon KS, Bae JS, Wagle S, Yu TK, Kim KM, Park HS, Lee J-H, Moon WS, Lee H, Chung MJ, Jang KY (2013) Expression of SIRT1 and DBC1 is associated with poor prognosis of soft tissue sarcomas. PLoS ONE 8(9):e74738. doi:10.1371/journal.pone.0074738
30. Roumes H, Leloup L, Dargelos E, Brustis J-J, Daury L, Cottin P (2010) Calpains: markers of tumor aggressiveness? Exp Cell Res 316:1587–1599