Inactivation of the Rgg2 transcriptional regulator ablates the virulence of Streptococcus pyogenes

PLoS ONE

Volumn 9, Issue 12, 2014, Pages

  Zutkis, Anastasia A ^a   Anbalagan, Srivishnupriya ^b   Chaussee, Michael S ^b   Dmitriev, Alexander V ^a,c  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Russian Federation)

^b UNIVERSITY OF SOUTH DAKOTA   (United States)

^c ST PETERSBURG STATE UNIVERSITY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; SIC PROTEIN; TRANSCRIPTOME; UNCLASSIFIED DRUG; MESSENGER RNA; RGG PROTEIN, STREPTOCOCCUS; TRANSACTIVATOR PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTEREMIA; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL VIRULENCE; BACTERICIDAL ACTIVITY; BACTERIUM ADHERENCE; BIOFILM; CONTROLLED STUDY; EPITHELIUM CELL; GENE EXPRESSION; GENE IDENTIFICATION; GENE INACTIVATION; GENE REPRESSION; GENETIC ASSOCIATION; GENETIC TRANSFORMATION; HUMAN; HUMAN CELL; MF 3 GENE; MOUSE; NGA GENE; NONHUMAN; NUCLEOTIDE METABOLISM; PHENOTYPE; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RGG2 GENE; SCPA GENE; SLO GEE; SSE GENE; STREPTOCOCCUS PYOGENES; TRANSCRIPTOMICS; ADULT; ANIMAL; ANTAGONISTS AND INHIBITORS; BLOOD; CELL CULTURE; CELL PROLIFERATION; FEMALE; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MICROBIOLOGY; MIDDLE AGED; PHYSIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; STREPTOCOCCAL INFECTIONS; VIRULENCE; WESTERN BLOTTING;

STREPTOCOCCUS PYOGENES;

ADULT; ANIMALS; BACTERIAL PROTEINS; BIOFILMS; BLOOD; BLOTTING, WESTERN; CELL PROLIFERATION; CELLS, CULTURED; EPITHELIAL CELLS; FEMALE; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; MICE; MIDDLE AGED; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SIGNAL TRANSDUCTION; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS PYOGENES; TRANS-ACTIVATORS; VIRULENCE;

EID: 84915751221     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0114784     Document Type: Article

References (28)

1. Kreikemeyer B, McIver KS, Podbielski A (2003) Virulence factor regulation and regulatory networks in Streptococcus pyogenes and their impact on pathogen-host interactions. Trends Microbiol 11(5): 224-232.
2. Chaussee MS, Ajdic D, Ferretti JJ (1999) The rgg gene of Streptococcus pyogenes NZ131 positively influences extracellular SPE B production. Infect Immun 67(4): 1715-1722.
3. Chang JC, LaSarre B, Jimenez JC, Aggarwal C, Federle MJ (2011) Two group A streptococcal peptide pheromones act through opposing Rgg regulators to control biofilm development. PLoS Pathog 8: e1002190.
4. Ferretti JJ, McShan WM, Ajdic D, Savic DJ, Savic G, et al. (2001) Complete genome sequence of an M1 strain of Streptococcus pyogenes. Proc Natl Acad Sci U S A 98(8): 4658-4663.
5. Fleuchot B, Gitton C, Guillot A, Vidic J, Nicolas P, et al. (2011) Rgg proteins associated with internalized small hydrophobic peptides: a new quorum-sensing mechanism in streptococci. Mol Microbiol 80(4): 1102-1119.
6. Ibrahim M, Nicolas P, Bessieres P, Bolotin A, Monnet V, et al. (2007) A genome-wide survey of short coding sequences in streptococci. Microbiology 153(11): 3631-3644.
7. Bortoni ME, Terra VS, Hinds J, Andrew PW, Yesilkaya H (2009) The pneumococcal response to oxidative stress includes a role for Rgg. Microbiology 155(12): 4123-4134.
8. Fontaine L, Boutry C, de Frahan MH, Delplace B, Fremaux C, et al. (2009) A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius. J Bacteriol 192(5): 1444-1454.
9. Mashburn-Warren L, Morrison DA, Federle MJ (2010) A novel double-tryptophan peptide pheromone controls competence in Streptococcus spp. via an Rgg regulator. Mol Microbiol 78(3): 589-606.
10. Morrison DA, Guédon E, Renault P (2013) Competence for natural genetic transformation in the Streptococcus bovis group streptococci S. infantarius and S. macedonicus. J Bacteriol 195(11): 2612-2620.
11. Polyakova EM, Mnatsakanyan ES, Burova LA, Dmitriev AV (2010) Genetic heterogeneity of emm12 genotype Streptococcus pyogenes strains. Med Acad J 10(1): 39-44 [in Russian].
12. Briko NI, Glushkova EV, Nosik AG, Dmitriev AV, Dmitrieva NF, Kleumenov DA, Lipatov KV (2014) The incidence of diseases caused by group A streptococci among invasive infections of soft tissues and characterization of causative agent. Zh Mikrobiol Epidemiol Immunobiol: in press. [in Russian].
13. Dmitriev AV, McDowell EJ, Kappeler KV, Chaussee MA, Chaussee MS (2006) The Rgg regulator of Streptococcus pyogenes influences utilization of nonglucose carbohydrates, prophage induction, and expression of the NAD-glycohydrolase virulence operon. J Bacteriol 188(20): 7230-7241.
14. Malke H, Mechold U, Gase K, Gerlach D (1994) Inactivation of the streptokinase gene prevents Streptococcus equisimilis H46A from acquiring cell-associated plasmin activity in the presence of plasminogen. FEMS Microbiol Lett 116: 107-112.
15. Kappeler KV, Anbalagan S, Dmitriev AV, McDowell EJ, Chaussee MS (2009) A naturally occurring Rgg variant in serotype M3 Streptococcus pyogenes does not activate speB expression due to altered specificity of DNA binding. Infect Immun 77(12): 5411-5417.
16. Bryan EM, Bae T, Kleerebezem M, Dunny GM (2000) Improved vectors for nisin-controlled expression in gram-positive bacteria. Plasmid 44: 183-190.
17. Doern CD, Roberts AL, Hong W, Nelson J, Lukomski S, et al. (2009) Biofilm formation by group A Streptococcus: a role for the streptococcal regulator of virulence (Srv) and streptococcal cysteine protease (SpeB). Microbiology 155(Pt 1): 46-52.
18. Chaussee MS, Somerville GA, Reitzer L, Musser JM (2003) Rgg coordinates virulence factor synthesis and metabolism in Streptococcus pyogenes. J Bacteriol 185(20): 6016-6024.
19. Anbalagan S, McShan WM, Dunman PM, Chaussee MS (2011) Identification of Rgg binding sites in the Streptococcus pyogenes chromosome. J Bacteriol 193(18): 4933-4942.
20. Watson ME Jr, Nielsen HV, Hultgren SJ, Caparon MG (2013) Murine vaginal colonization model for investigating asymptomatic mucosal carriage of Streptococcus pyogenes. Infect Immun. 81(5): 1606-1617.
21. Ricker A, Vickerman M, Dongari-Bagtzoglou A (2014) Streptococcus gordonii glucosyltransferase promotes biofilm interactions with Candida albicans. J Oral Microbiol 6: 23419.
22. Chaussee MA, Callegari EA, Chaussee MS (2004) Rgg regulates growth phase-dependent expression of proteins associated with secondary metabolism and stress in Streptococcus pyogenes. J Bacteriol 186(21): 7091-7099.
23. Dmitriev AV, McDowell EJ, Chaussee MS (2008) Inter- and intraserotypic variation in the Streptococcus pyogenes Rgg regulon. FEMS Microbiol Lett 284(1): 43-51.
24. Joo HS, Otto M (2012) Molecular basis of in vivo biofilm formation by bacterial pathogens. Chem Biol 19(12): 1503-1513.
25. Cook LC, Federle MJ (2014) Peptide pheromone signaling in Streptococcus and Enterococcus. FEMS Microbiol Rev 38(3): 473-492.
26. Jewett MW, Lawrence KA, Bestor A, Byram R, Gherardini F, et al. (2009) GuaA and GuaB are essential for Borrelia burgdorferi survival in the tick-mouse infection cycle. J Bacteriol 191(20): 6231-6241.
27. Wang JY, Pasetti MF, Noriega FR, Anderson RJ, Wasserman SS, et al. (2001) Construction, genotypic and phenotypic characterization, and immunogenicity of attenuated DeltaguaBA Salmonella enterica serovar Typhi strain CVD 915. Infect Immun 69(8): 4734-4741.
28. Santiago AE, Cole LE, Franco A, Vogel SN, Levine MM, et al. (2009) Characterization of rationally attenuated Francisella tularensis vaccine strains that harbor deletions in the guaA and guaB genes. Vaccine 27(18): 2426-2436.