Development of a sensitive potency assay to measure the anti-proliferation effect of an anti-HER2 antibody

Journal of Immunological Methods

Volumn 415, Issue , 2014, Pages 80-85

  Lu, Xiaohui ^a   Bergelson, Svetlana ^a  

^a BIOGEN   (United States)

Author keywords

BrdU incorporation assay; DELFIA; Potency assay

Indexed keywords

ANTINEOPLASTIC AGENT; EPIDERMAL GROWTH FACTOR RECEPTOR 2 ANTIBODY; EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODY; UNCLASSIFIED DRUG; ADENOSINE TRIPHOSPHATE; BROXURIDINE; EPIDERMAL GROWTH FACTOR RECEPTOR 2; ERBB2 PROTEIN, HUMAN; FLUORESCENT DYE; MONOCLONAL ANTIBODY; OXAZINE DERIVATIVE; RESAZURIN; XANTHENE DERIVATIVE;

ANTIPROLIFERATIVE ACTIVITY; ARTICLE; BIOLUMINESCENCE; CANCER INHIBITION; CELL COUNT; CELL DENSITY; CELL POPULATION; CELL PROLIFERATION; CELL PROLIFERATION ASSAY; CELL VIABILITY; CHEMOLUMINESCENCE; COLORIMETRY; CONTROLLED STUDY; DRUG DETERMINATION; DRUG POTENCY; HUMAN; HUMAN CELL; IMMUNOASSAY; RESAZURIN ASSAY; ANTAGONISTS AND INHIBITORS; BIOSYNTHESIS; DRUG EFFECTS; FACTORIAL ANALYSIS; GENE EXPRESSION; GENETICS; IMMUNOLOGY; LUMINESCENCE; METABOLISM; PROCEDURES; REPRODUCIBILITY; SIGNAL NOISE RATIO; STANDARDS; TUMOR CELL LINE;

ADENOSINE TRIPHOSPHATE; ANTIBODIES, MONOCLONAL; BROMODEOXYURIDINE; CELL LINE, TUMOR; CELL PROLIFERATION; FACTOR ANALYSIS, STATISTICAL; FLUORESCENT DYES; GENE EXPRESSION; HUMANS; IMMUNOASSAY; LUMINESCENT MEASUREMENTS; OXAZINES; RECEPTOR, ERBB-2; REPRODUCIBILITY OF RESULTS; SIGNAL-TO-NOISE RATIO; XANTHENES;

EID: 84913536618     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2014.10.007     Document Type: Article

References (8)

1. Ahmed S.A., Gogal R.M., Walsh J.E. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to 3H-thymidine incorporation assay. J. Immunol. Methods 1994, 170:211.
2. Baselga J., Albanell J. Mechanism of action of anti-HER2 monoclonal antibodies. Ann. Oncol. 2001, 12:S35.
3. Crouch S., Kozlowski R., Slater K., Fletscher J. The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. J. Immunol. Methods 1993, 160:81.
4. International Conference on Harmonisation (ICH) of Technical Requirement for Registration of Pharmaceuticals for Human Use, Specifications: Test Procedures and Acceptance Criteria for Biotechnological / Biological Products, Q6B, CPMP/ICH/365/96 1999, 1.
5. Lane H.A., Beuvink I., Motoyama A.B., Daly J.M., Neve R.M., Hynes N.E. ErbB2 potentiates breast tumor proliferation through modulation of p27Kip1-Cdk2 complex formation: receptor overexpression does not determine growth dependency. Mol. Cell. Biol. 2000, 20:3210.
6. Mosmann T. Rapid calorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65:55.
7. Porstmann T., Ternynck T., Avrameas S. Quantitation of 5-bromo-2-deoxyuridine incorporation into DNA: an enzyme immunoassay for the assessment of the lymphoid cell proliferative response. J. Immunol. Methods 1985, 82:169.
8. Zumpea C., Bachmanna C.L., Metzgera A.U., Wiedemanna N. Comparison of potency assays using different read-out systems and their suitability for quality control. J. Immunol. Methods 2010, 360:P129.