Comparison of several methods for the separation of poly(3-hydroxybutyrate) from Cupriavidus necator H16 cultures

Biochemical Engineering Journal

Volumn 93, Issue , 2015, Pages 250-259

  Lopez Abelairas M ^a   Garcia Torreiro M ^a   Lu Chau T ^a   Lema, J M ^a   Steinbuchel A ^b,c  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^b UNIVERSITY OF MÜNSTER   (Germany)

^c KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

Author keywords

Bioseparation; Cell disruption; Cupriavidus necator; Downstream processing; Polyhydroxyalkanoates; Purification

Indexed keywords

BIOMOLECULES; BIOPOLYMERS; CARBON DIOXIDE; CAUSTIC SODA; COST EFFECTIVENESS; COSTS; ENVIRONMENTAL IMPACT; HALOGENATION; HYDROMETALLURGY; RECOVERY; SODIUM; SULFURIC ACID;

BIO-SEPARATION; CELL DISRUPTION; CUPRIAVIDUS NECATOR; DOWNSTREAM-PROCESSING; POLYHYDROXYALKANOATES;

PURIFICATION;

BIOPOLYMER; CARBON DIOXIDE; HYPOCHLORITE SODIUM; POLY(3 HYDROXYBUTYRIC ACID); SODIUM HYDROXIDE; SULFURIC ACID;

ARTICLE; BIOREACTOR; CONTROLLED STUDY; CUPRIAVIDUS NECATOR; DECOMPOSITION; ENVIRONMENTAL IMPACT; GREENHOUSE; MELTING POINT;

CUPRIAVIDUS NECATOR;

EID: 84911887067     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2014.10.018     Document Type: Article

References (30)

1. Steinbüchel A., Schlegel H.G. Physiology and molecular genetics of poly(β-hydroxyalkanoic acid) synthesis in Alcaligenes eutrophus. Mol. Microbiol. 1991, 5:535-542.
2. Steinbüchel A., Valentin H.E. Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiol. Lett. 1995, 128:219-228.
3. Ling Y., Williams D.R.G., Thomas C.J., Middelberg A.P.J. Recovery of poly-3-hydroxybutyrate from recombinant Escherichia coli by homogenization and centrifugation. Biotechnol. Tech. 1997, 11:409-412.
4. Jacquel N., Lo C.-W., Wei Y.-H., Wu H.-S., Wang S.S. Isolation and purification of bacterial poly(3-hydroxyalkanoates). Biochem. Eng. J. 2008, 39:15-27.
5. Ryu H.W., Cho K.-S., Lee E.G., Chang Y.K. Recovery of poly(3-hydroxybutyrate) from coagulated Ralstonia eutropha using a chemical digestion method. Biotechnol. Prog. 2000, 16:676-679.
6. Lo C.-W., Wu H.-S., Wei Y.-H. High throughput study of separation of poly(3-hydroxybutyrate) from recombinant Escherichia coli XL1 blue. J. Taiwan Inst. Chem. Eng. 2011, 42:240-246.
7. Anis S.N.S., Nurhezreen M.I., Sudesh K., Amirul A.A. Enhanced recovery and purification of P(3HB-co-3HHx) from recombinant Cupriavidus necator using alkaline digestion method. Appl. Biochem. Biotechnol. 2012, 167:524-535.
8. Madkour M.H., Heinrich D., Alghamdi M.A., Shabbaj I.I., Steinbüchel A. PHA recovery from biomass. Biomacromolecules 2013, 10.1021/bm4010244.
9. Yu J., Chen L.X.L. Cost-effective recovery and purification of polyhydroxyalkanoates by selective dissolution of cell mass. Biotechnol. Prog. 2006, 22:547-553.
10. Schlegel H.G., Kaltwasser H., Gottschalk G. Ein Submersverfahren zur Kultur wasserstoffoxidierender Bakterien: wachstumsphysiologische Untersuchungen. Arch. Mikrobiol. 1961, 38:209-222.
11. Heinrich D., Madkour M.H., Al-467 Ghamdi M.A., Shabbaj I.I., Steinbüchel A. Large scale extraction of poly(3-hydroxybutyrate) from Ralstonia eutropha H16 using sodium hypochlorite. AMB Express 2012, 2:59.
12. Yellore V., Desai A. Production of poly-3-hydroxybutyrate from lactose and whey by Methylobacterium sp. ZP24. Lett. Appl. Microbiol. 1998, 26:391-394.
13. Brandl H., Gross R.A., Lenz R.W., Fuller R.C. Pseudomonas oleovorans as a source of poly(β-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl. Environ. Microbiol. 1988, 54:1977-1982.
14. Timm A., Byrom D., Steinbüchel A. Formation of blends of various poly(3-hydroxyalkanoic acids) by a recombinant strain of Pseudomonas oleovorans. Appl. Microbiol. Biotechnol. 1990, 33:296-301.
15. Barham P.J., Keller A., Otun E.L. Crystallization and morphology of a bacterial thermoplastic: poly-3-hydroxybutyrate. J. Mater. Sci. 1984, 19:2781-2794.
16. Williamson D.H., Wilkinson J.F. The isolation and estimation of the poly-β-hydroxy-butyrate inclusions of Bacillus species. J. Gen. Microbiol. 1958, 19:198-209.
17. Berger E., Ramsay B.A., Ramsay J.A., Chavarie C., Braunegg G. PHB recovery by hypochlorite digestion of non-PHB biomass. Biotechnol. Tech. 1989, 3:227-232.
18. Hahn S.K., Chang Y.K., Kim B.S., Chang H.N. Communication to the editor optimization of microbial poly (3-hydroxybutyrate) recovery using dispersions of sodium hypochlorite solution and chloroform. Biotechnol. Bioeng. 1994, 44:256-261.
19. Lu C.H. Purification and Separation of Polyhydroxyalkanoates from Bacteria 2006, Yuan Ze University, Taiwan, (M.S. Thesis).
20. Jendrossek D., Müller B., Schlegel H.G. Cloning and characterization of the poly(hydroxyalkanoic acid)-depolymerase gene locus, phaZ1 of Pseudomonas lemoignei and its gene product. Eur. J. Biochem. 1993, 218:701-710.
21. Yu J., Plackett D., Chen L.X.L. Kinetics and mechanism of the monomeric products from abiotic hydrolysis of poly[(R)-3-hydroxybutyrate] under acidic and alkaline conditions. Polym. Degrad. Stab. 2005, 89:289-299.
22. Penloglou G., Kretza E., Chatzidoukas C., Parouti S., Kiparissides C. On the control of molecular weight distribution of polyhydroxybutyrate in Azohydromonas lata cultures. Biochem. Eng. J. 2012, 62:39-47.
23. Choi J., Lee S.Y. Factors affecting the economics of polyhydroxyalkanoate production by bacterial fermentation. Appl. Microbiol. Biotechnol. 1999, 51:13-21.
24. Hong S.-G., Lin Y.-C., Lin C.-H. Crystallization and degradation behaviors of treated polyhydroxybutyrates. React. Funct. Polym. 2008, 68(11):1516-1523.
25. Lauzier C., Marchessault R.H., Smith P., Chanzy H. Structural study of isolated poly(β-hydroxybutyrate) granules. Polymer 1992, 33(4):823-827.
26. Abdel Ghaffar A.M. Development of a Biodegradable Material Based on Poly(3-Hydroxybutyrate) (PHB) 2002, Martin Luther Universität Halle, (Ph.D. Thesis).
27. Arza C.R., Jannasch P., Maurer F.H.D. Network formation of graphene oxide in poly(3-hydroxybutyrate) nanocomposites. Eur. Polym. J. 2014, 59:262-269.
28. Torres J.M., Stafford C.M., Vogt B.D. Impact of molecular mass on the elastic modulus of thin polystyrene films. Polymer 2010, 51(18):4211-4217.
29. Chen Y., Xu Q., Yang H., Gu G. Effects of cell fermentation time and biomass drying strategies on the recovery of poly-3-hydroxyalkanoates from Alcaligenes eutrophus using a surfactant-chelate aqueous system. Process Biochem. 2001, 36:773-779.
30. Yang Y.-H., Brigham C., Willis L., Rha C., Sinskey A. Improved detergent-based recovery of polyhydroxyalkanoates (PHAs). Biotechnol. Lett. 2011, 33:937-942.