Phenotypic and genotypic diversity of multidrug-resistant Pseudomonas aeruginosa isolates from bloodstream infections recovered in the hospitals of Belo Horizonte, Brazil

Chemotherapy

Volumn 60, Issue 1, 2014, Pages 54-62

  Inacio, Hanoch S M ^a   Bomfim, Maria Rosa Q ^b   França, Rafaela O ^a   Farias, Luiz M ^a   Carvalho, Maria Auxiliadora R ^a   Serufo, José Carlos ^a   Santos, Simone Gonçalves ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b CEUMA UNIVERSITY   (Brazil)

Author keywords

Bloodstream infections; Genetic diversity; Pseudomonas aeruginosa; Resistance markers

Indexed keywords

AMIKACIN; AZTREONAM; BACTERIAL ENZYME; BETA LACTAMASE; CEFEPIME; CEFTAZIDIME; CEPHALOSPORINASE; CIPROFLOXACIN; GENTAMICIN; IMIPENEM; LEVOFLOXACIN; MEROPENEM; METALLO BETA LACTAMASE; OXACILLINASE; PIPERACILLIN PLUS TAZOBACTAM; POLYMYXIN B; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; BACTERIAL DNA; METALLO-BETA-LACTAMASE (VIM-13), PSEUDOMONAS AERUGINOSA;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BLOOD CULTURE; BLOODSTREAM INFECTION; BRAZIL; CONTROLLED STUDY; DISK DIFFUSION; ENTEROBACTERIAL REPETITIVE INTERGENIC CONSENSUS POLYMERASE CHAIN REACTION; ENZYME SYNTHESIS; EPSILOMETER TEST; GENE AMPLIFICATION; GENETIC VARIABILITY; HUMAN; MULTIDRUG RESISTANCE; NONHUMAN; NUCLEIC ACID ANALYSIS; PHENOTYPIC VARIATION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; RANDOM AMPLIFIED POLYMORPHIC DNA; DRUG EFFECTS; GENETICS; GENOTYPE; HOSPITAL; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; PATHOLOGY; PHENOTYPE; PSEUDOMONAS INFECTION;

ANTI-INFECTIVE AGENTS; BETA-LACTAMASES; BRAZIL; CEPHALOSPORINASE; DISK DIFFUSION ANTIMICROBIAL TESTS; DNA, BACTERIAL; DRUG RESISTANCE, MULTIPLE, BACTERIAL; GENOTYPE; HOSPITALS; HUMANS; PHENOTYPE; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE;

EID: 84911479032     PISSN: 00093157     EISSN: 14219794     Source Type: Journal    
DOI: 10.1159/000365726     Document Type: Article

References (30)

1. Zilberberg MD, Shorr AF: Prevalence of multidrug-resistant Pseudomonas aeruginosa and carbapenem-resistant enterobacteriaceae among specimens from hospitalized patients with pneumonia and bloodstream infections in the United States from 2000 to 2009. J Hosp Med 2013; 8: 559-563.
2. Walsh TR, Toleman MA, Hryniewicz W, Bennett PM, Jones RN: Evolution of an integron carrying blaVIM-2 in Eastern Europe: report from the SENTRY Antimicrobial Surveillance Program. J Antimicrob Chemother 2003; 52: 116-119.
3. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, Paterson DL, Rice LB, Stelling J, Struelens MJ, Vatopoulos A, Weber JT, Monnet DL: Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 2012; 18: 268-281.
4. Queenan AM, Bush K: Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev 2007; 20: 440-458.
5. Bowers DR, Liew YX, Lye DC, Kwa AL, Hsu LY, Tam VH: Outcomes of appropriate empiric combination versus monotherapy for Pseudomonas aeruginosa bacteremia. Antimicrob Agents Chemother 2013; 57; 1270-1274.
6. Hammami S, Boutiba-Ben Boubaker I, Ghozzi R, Saidani M, Amine S, Ben Redjeb S: Nosocomial outbreak of imipenem-resistant Pseudomonas aeruginosa producing VIM-2 metallo-beta-lactamase in a kidney transplantation unit. Diagn Pathol 2011; 6: 106.
7. Yan JJ, Hsueh PR, Ko WC, Luh KT, Tsai SH, Wu HM, Wu JJ: Metallo-beta-lactamases in clinical Pseudomonas isolates in Taiwan and identification of VIM-3, a novel variant of the VIM-2 enzyme. Antimicrob Agents Chemother 2001; 45: 2224-2228.
8. Speijer H, Savelkoul PHM, Bonten MJ, Stobberingh EE, Tjhie JHT: Application of different genotyping methods for Pseudomonas aeruginosa in a setting of endemicity in an intensive care unit. J Clin Microbiol 1999; 37: 3654-3661.
9. Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing, 17th informational supplement, 2011.
10. Martins HS, Bomfim MRQ, França RO, Farias LM, Carvalho MAR, Serufo JC, Santos SG: Resistance markers and genetic diversity in Acinetobacter baumannii strains recovered from nosocomial bloodstream infections. Int J Environ Res Public Health 2014; 11: 1465-1478.
11. Dalmarco EM, Blatt SL, Córdova CMM: Identificação laboratorial de β-lactamases de espectro estendido (ESBLs). Ver Bras Anal Clin 2006; 38: 171-177.
12. Lee K, Yum JH, Yong D, Lee HM, Kim HD, Docquier JD, Rossolini GM, Chong Y: Novel acquired metallo-beta-lactamase gene, bla(SIM-1), in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. Antimicrob Agents Chemother 2005; 49: 4485-4491.
13. Coudron PE, Moland ES, Thomson KS: Occurrence and detection of AmpC beta-lactamases among Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates at a veterans medical center. J Clin Microbiol 2000; 38: 1791-1796.
14. Woodford N, Ellington MJ, Coelho JM, Turton JF, Ward ME, Brown S, Amyes SGB, Livermore DM: Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Ag 2006; 27: 351-353.
15. Silbert S, Pfaller MA, Hollis RJ, Barth AL, Sader HS: Evaluation of three molecular typing techniques for nonfermentative Gram-negative bacilli. Infect Control Hosp Epidemiol 2004; 25: 847-851.
16. Versalovic J, Koeuth T, Lupski JR: Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 1991; 19: 6823-6831.
17. Dabrowski W, Czekajlo-Kolodziej U, Medrala D, Giedrys-Kalemba S: Optimisation of AP-PCR fingerprinting discriminatory power for clinical isolates of Pseudomonas aeruginosa. FEMS Microbiol Lett 2003; 218: 51-57.
18. Sanguinetti CJ, Dias Neto E, Simpson AJG: Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotech 1994; 17: 915-918.
19. Ribeiro J, Mendes RE, Domingos R, Franca E, Silbert S, Jones RN, Sader HS: Microbiological and epidemiological characterization of imipenem-resistant Pseudomonas aeruginosa strains from a Brazilian tertiary hospital: report from the SENTRY Antimicrobial Surveillance Program. J Chemother 2006; 18: 461-467.
20. Machado GM, Lago A, Fuentefria SR, Fuentefria DB: Occurrence and the susceptibility to antimicrobial agents in Pseudomonas aeruginosa and Acinetobacter sp. at a tertiary hospital in southern Brazil. Rev Soc Bras Med Trop 2011; 44: 168-172.
21. Llaca-Díaz JM, Mendonza-Olazarán S, Camacho-Ortiz A, Flores S, Garza-González E: One-year surveillance of ESKAPE pathogens in an intensive care unit in Monterrey, Mexico. Chemotherapy 2012; 58: 475-481.
22. Cornaglia G, Giamarellou H, Rossolini GM: Metallo-beta-lactamases: a last frontier for beta-lactams? Lancet Infect Dis 2011; 11: 381-393.
23. Livermore DM: Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa : our worst nightmare? Clin Infect Dis 2002; 34: 634-640.
24. Falagas ME, Rafailidis PI, Matthaiou DK: Resistance to polymyxins: Mechanisms, frequency and treatment options. Drug Resist Updat 2010; 13: 132-138.
25. Qu T, Zhang J, Wang J, Tao J, Yu Y, Chen Y, Zhou J, Li L: Evaluation of phenotypic tests for detection of metallo-β-lactamase-producing Pseudomonas aeruginosa strains in China. J Clin Microbiol 2009; 47: 1136-1142.
26. Picão RC, Carrara-Marroni FE, Gales AC, Venâncio EJ, Xavier DE, Tognim MC, Pelavo JS: Metallo-β-lactamase-production in meropenem susceptible Pseudomonas aeruginosa isolates: risk for silent spread. Mem Inst Oswaldo Cruz 2012; 107: 747-751.
27. Speert DP: Molecular epidemiology of Pseudomonas aeruginosa. Front Biosci 2002; 1: 354-361.
28. Cezario RC, Duarte De Morais L, Ferreira JC, Costa-Pinto RM, da Costa Darini AL, Gontijo-Filho PP: Nosocomial outbreak by imipenem-resistant metallo-beta-lactamase-producing Pseudomonas aeruginosa in an adult intensive care unit in a Brazilian teaching hospital. Enferm Infecc Microbiol Clin 2009; 27: 269-274.
29. Toleman MA, Simm AM, Murphy TA, Gales AC, Biedenbach DJ, Jones RN, Walsh TR: Molecular characterization of SPM-1, a novel metallo-beta-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme. J Antimicrob Chemother 2002; 50: 673-679.
30. Franco MR, Caiaffa-Filho HH, Burattini MN, Rossi F: Metallo-beta-lactamases among imipenem-resistant Pseudomonas aeruginosa in a Brazilian university hospital. Clinics 2010; 65: 825-829.