In vitro culture of mouse blastocysts beyond the implantation stages

Nature Protocols

Volumn 9, Issue 12, 2014, Pages 2732-2739

  Bedzhov, Ivan ^a,b   Leung, Chuen Yan ^a,b   Bialecka, Monika ^a,b   Zernicka Goetz, Magdalena ^a,b  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL TISSUE; ARTICLE; BLASTOCYST; CELL ADHESION; CELL DIFFERENTIATION; CELL PROLIFERATION; COCULTURE; CONTROLLED STUDY; EMBRYO; EMBRYO CULTURE; EMBRYO GROWTH; FEMALE; FIBROBLAST; GAS EXCHANGE; GIANT CELL; IN VITRO STUDY; MOUSE; NIDATION; NONHUMAN; VERO CELL LINE; ANIMAL; C57BL MOUSE; CYTOLOGY; DEVICES; ECTODERM; PHYSIOLOGY; PROCEDURES;

ANIMALS; BLASTOCYST; CELL ADHESION; ECTODERM; EMBRYO CULTURE TECHNIQUES; EMBRYO IMPLANTATION; FEMALE; MICE, INBRED C57BL;

EID: 84911468282     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.186     Document Type: Article

References (24)

1. Ma, W.-G., Song, H., Das, S.K., Paria, B.C. & Dey, S.K. Estrogen is a critical determinant that specifies the duration of the window of uterine receptivity for implantation. Proc. Natl. Acad. Sci. 100, 2963-2968 (2003).
2. Song, H., Han, K. & Lim, H. Progesterone supplementation extends uterine receptivity for blastocyst implantation in mice. Reproduction 133, 487-493 (2007).
3. Parr, E.L., Tung, H.N. & Parr, M.B. Apoptosis as the mode of uterine epithelial cell death during embryo implantation in mice and rats. Biol. Reprod. 36, 211-225 (1987).
4. Ansell, J.D., Barlow, P.W. & McLaren, A. Binucleate and polyploid cells in the decidua of the mouse. J. Embryol. Exp. Morphol. 31, 223-227 (1974).
5. Bedzhov, I. & Zernicka-Goetz, M. Self-organizing properties of mouse pluripotent cells initiate morphogenesis upon implantation. Cell 156, 1032-1044 (2014).
6. Jenkinson, E.J. & Wilson, I.B. In vitro support system for the study of blastocyst differentiation in the mouse. Nature 228, 776-778 (1970).
7. Copp, A.J. The mechanism of mouse eg g-cylinder morphogenesis in vitro. J. Embryol. Exp. Morphol. 61, 277-287 (1981).
8. Hsu, Y.C. Differentiation in vitro of mouse embryos beyond the implantation stage. Nature 239, 200-202 (1972).
9. Solter, D., Biczysko, W., Pienkowski, M. & Koprowski, H. Ultrastructure of mouse egg cylinders developed in vitro. Anat. Rec. 180, 263-279 (1974).
10. Wiley, L. M. & Pedersen, R.A. Morphology of mouse egg cylinder development in vitro: a light and electron microscopic study. J. Exp. Zool. 200, 389-402 (1977).
11. Hsu, Y.C. In vitro development of individually cultured whole mouse embryos from blastocyst to early somite stage. Dev. Biol. 68, 453-461 (1979).
12. Morris, S.A. et al. Dynamics of anterior-posterior axis formation in the developing mouse embryo. Nat. Commun. 3, 673 (2012).
13. Wu, T.C., Wan, Y.J. & Damjanov, I. Positioning of inner cell mass determines the development of mouse blastocysts in vitro. J. Embryol. Exp. Morphol. 65, 105-117 (1981).
14. Carson, D.D., Tang, J.P. & Gay, S. Collagens support embryo attachment and outgrowth in vitro: effects of the Arg-Gly-Asp sequence. Dev. Biol. 127, 368-375 (1988).
15. Hsu, Y.C. Differentiation in vitro of mouse embryos to the stage of early somite. Dev. Biol. 33, 403-411 (1973).
16. Salomon, D.S. & Sherman, M.I. Implantation and invasiveness of mouse blastocysts on uterine monolayers. Exp. Cell Res. 90, 261-268 (1975).
17. Kauma, S.W. & Matt, D.W. Coculture cells that express leukemia inhibitory factor (LIF) enhance mouse blastocyst development in vitro. J. Assist. Reprod. Gen. 12, 153-156 (1995).
18. Gonda, M.A. & Hsu, Y.-C. Correlative scanning electron, transmission electron, and light microscopic studies of the in vitro development of mouse embryos on a p lastic substrate at the implantation stage. J. Embryol. Exp. Morphol. 56, 23-39 (1980).
19. Olovsson, M. & Nilsson, B.O. Structural and functional properties of trophoblast cells of mouse egg-cylinders in vitro. Anat. Rec. 236, 417-424 (1993).
20. Hsu, Y.C. In vitro development of whole mouse embryos beyond the implantation stage. in Methods in Mammalian Reproduction (ed. Daniel, J.C.) 229-245 (Academic Press, 1978).
21. Wilson, I.B. & Jenkinson, E.J. Blastocyst differentiation in vitro. J. Reprod. Fertil. 39, 243-249 (1974).
22. Tam, P.P. Postimplantation mouse development: whole embryo culture and micro-manipulation. Int. J. Dev. Biol. 42, 895-902 (1998).
23. Behringer, R., Gertsenstein, M., Nagy, K.V. & Nagy, A. Manipulating the Mouse Embryo: a Laboratory Manual 4th ed. (Cold Spring Harbor Laboratory Press, 2014)
24. Mesnard, D., Filipe, M., Belo, J.A. & Zernicka-Goetz, M. The anterior-posterior axis emerges respecting the morphology of the mouse embryo that changes and aligns with the uterus before gastrulation. Curr. Bio l. 14, 184-196 (2004).