Comparison of sputum and nasopharyngeal swabs for detection of respiratory viruses

Journal of Medical Virology

Volumn 86, Issue 12, 2014, Pages 2122-2127

  Jeong, Ji Hun ^a   Kim, Kyung Hee ^a   Jeong, Sung Hwan ^a   Park, Jeong Woong ^a   Lee, Sang Min ^a   Seo, Yiel Hea ^a  

^a Gachon University College of Medicine   (South Korea)

Author keywords

Nasopharyngeal swab; Reverse transcription polymerase chain reaction; Sputum

Indexed keywords

ADENOVIRIDAE; ADULT; ARTICLE; CHRONIC LUNG DISEASE; COMPARATIVE STUDY; CONTROLLED STUDY; CORONAVIRUS; DIAGNOSTIC VALUE; FEMALE; HUMAN; HUMAN RESPIRATORY SYNCYTIAL VIRUS A; HUMAN RHINOVIRUS; INFLUENZA A; INFLUENZA VIRUS A; MAJOR CLINICAL STUDY; MALE; NASOPHARYNGEAL SWAB; NUCLEIC ACID AMPLIFICATION; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY VIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SMEAR; SPUTUM; VIRAL RESPIRATORY TRACT INFECTION; VIRUS DETECTION; DIAGNOSTIC PROCEDURE; EVALUATION STUDY; ISOLATION AND PURIFICATION; MIDDLE AGED; MULTIPLEX POLYMERASE CHAIN REACTION; NASOPHARYNX; PROCEDURES; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY; VIROLOGY; VIRUS;

ADULT; CLINICAL LABORATORY TECHNIQUES; FEMALE; HUMANS; MALE; MIDDLE AGED; MULTIPLEX POLYMERASE CHAIN REACTION; NASOPHARYNX; RESPIRATORY TRACT INFECTIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPUTUM; VIROLOGY; VIRUSES;

EID: 84911421338     PISSN: 01466615     EISSN: 10969071     Source Type: Journal    
DOI: 10.1002/jmv.23937     Document Type: Article

References (16)

1. Bogoch II, Andrews JR, Zachary KC, Hohmann EL. 2013. Diagnosis of influenza from lower respiratory tract sampling after negative upper respiratory tract sampling. Virulence 4:82-84.
2. Desjardin L, Perkins M, Teixeira L, Cave M, Eisenach K. 1996. Alkaline decontamination of sputum specimens adversely affects stability of mycobacterial mRNA. J Clin Microbiol 34:2435-2439.
3. Drews SJ, Blair J, Lombos E, DeLima C, Burton L, Mazzulli T, Low DE. 2008. Use of the Seeplex RV Detection kit for surveillance of respiratory viral outbreaks in Toronto, Ontario, Canada. Ann Clin Lab Sci 38:376-379.
4. Falsey AR, Formica MA, Walsh EE. 2012. Yield of sputum for viral detection by reverse transcriptase PCR in adults hospitalized with respiratory illness. J Clin Microbiol 50:21-24.
5. Gharabaghi F, Hawan A, Drews S, Richardson S. 2011. Evaluation of multiple commercial molecular and conventional diagnostic assays for the detection of respiratory viruses in children. Clin Microbiol Infect 17:1900-1906.
6. Kim SR, Ki CS, Lee NY. 2009. Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay. J Virol Methods 156:111-116.
7. Landis JR, Koch GG. 1977. The measurement of observer agreement for categorical data. Biometrics 33:159-174.
8. Lieberman D, Shimoni A, Keren-Naus A, Steinberg R, Shemer-Avni Y. 2009. Identification of respiratory viruses in adults: Nasopharyngeal versus oropharyngeal sampling. J Clin Microbiol 47:3439-3443.
9. Loens K, Van Heirstraeten L, Malhotra-Kumar S, Goossens H, Ieven M. 2009. Optimal sampling sites and methods for detection of pathogens possibly causing community-acquired lower respiratory tract infections. J Clin Microbiol 47:21-31.
10. Monto AS. 2002. Epidemiology of viral respiratory infections. Am J Med 112:4-12.
11. Pérez-Ruiz M, Pedrosa-Corral I, Sanbonmatsu-Gámez S, Navarro-Marí M. 2012. Laboratory detection of respiratory viruses by automated techniques. Open Virol J 6:151-159.
12. Roa PL, Rodríguez-Sánchez B, Catalán P, Giannella M, Alcalá L, Padilla B, Viedma DG, Muñoz P, Bouza E. 2012. Diagnosis of influenza in intensive care units: Lower respiratory tract samples are better than nose-throat swabs. Am J Respir Crit Care Med 186:929-930.
13. Storch GA. 2000. Diagnostic virology. Clin Infect Dis 31:739-751.
14. Xiang X, Qiu D, Hegele RD, Tan WC. 2001. Comparison of different methods of total RNA extraction for viral detection in sputum. J Virol Methods 94:129-135.
15. Yu X, Lu R, Wang Z, Zhu N, Wang W, Julian D, Chris B, Lu J, Tan W. 2012. Etiology and clinical characterization of respiratory virus infections in adult patients attending an emergency department in Beijing. PLoS ONE 7:e32174.
16. Zhang G, Hu Y, Wang H, Zhang L, Bao Y, Zhou X. 2012. High incidence of multiple viral infections identified in upper respiratory tract infected children under three years of age in Shanghai, China. PloS one 7:e44568.