A parallel diagnostic accuracy study of three molecular panels for the detection of bacterial gastroenteritis

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 33, Issue 11, 2014, Pages 2075-2081

  Biswas, J S ^a   Al Ali, A ^b   Rajput, P ^b   Smith, D ^b   Goldenberg, S D ^a,c  

^a GUY'S AND ST THOMAS' NHS FOUNDATION TRUST   (United Kingdom)

^b ViaPath Pathology LLP   (United Kingdom)

^c ST THOMAS HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; COMPARATIVE STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; FECES ANALYSIS; GASTROENTERITIS; HUMAN; NONHUMAN; SENSITIVITY AND SPECIFICITY; TURNAROUND TIME; ADULT; BACTERIAL INFECTIONS; BACTERIUM; COHORT ANALYSIS; DIAGNOSTIC TEST; EVALUATION STUDY; FECES; FEMALE; GENETICS; ISOLATION AND PURIFICATION; MALE; MICROBIOLOGY; MIDDLE AGED; MOLECULAR DIAGNOSIS; PROCEDURES; RETROSPECTIVE STUDY; TIME; YOUNG ADULT;

ADULT; BACTERIA; BACTERIAL INFECTIONS; COHORT STUDIES; DIAGNOSTIC TESTS, ROUTINE; FECES; FEMALE; GASTROENTERITIS; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; RETROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; TIME FACTORS; YOUNG ADULT;

EID: 84911005340     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-014-2177-9     Document Type: Article

References (18)

1. Tam CC, Rodrigues LC, Viviani L, Dodds JP, Evans MR, Hunter PR et al (2012) Longitudinal study of infectious intestinal disease in the UK (IID2 study): incidence in the community and presenting to general practice. Gut 61(1):69–77
2. Haagsma JA, Geenen PL, Ethelberg S, Fetsch A, Hansdotter F, Jansen A et al (2013) Community incidence of pathogen-specific gastroenteritis: reconstructing the surveillance pyramid for seven pathogens in seven European Union member states. Epidemiol Infect 141:1625–1639
3. Halligan E, Edgeworth J, Bisnauthsing K, Bible J, Cliff P, Aarons E et al (2013) Multiplex molecular testing for management of infectious gastroenteritis in a hospital setting: a comparative diagnostic and clinical utility study. Clin Microbiol Infect (in press)
4. Amar CF, East CL, Gray J, Iturriza-Gomara M, Maclure EA, McLauchlin J (2007) Detection by PCR of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the English case–control infectious intestinal disease study (1993–1996). Eur J Clin Microbiol Infect Dis 26(5):311–323
5. de Boer RF, Ott A, Kesztyüs B, Kooistra-Smid AM (2010) Improved detection of five major gastrointestinal pathogens by use of a molecular screening approach. J Clin Microbiol 48(11):4140–4146
6. Wessels E, Rusman LG, van Bussel MJ, Claas EC (2014) Added value of multiplex Luminex gastrointestinal pathogen panel (xTAG® GPP) testing in the diagnosis of infectious gastroenteritis. Clin Microbiol Infect 20:O182–O187
7. Anderson NW, Buchan BW, Ledeboer NA (2014) Comparison of the BD MAX™ enteric bacterial panel to routine culture methods for detection of Campylobacter, enterohemorrhagic Escherichia coli (O157), Salmonella, and Shigella isolates in preserved stool specimens. J Clin Microbiol 52(4):1222–1224. doi:10.1128/JCM.03099-13
8. Mengelle C, Mansuy JM, Prere MF, Grouteau E, Claudet I, Kamar N et al (2013) Simultaneous detection of gastrointestinal pathogens with a multiplex Luminex-based molecular assay in stool samples from diarrhoeic patients. Clin Microbiol Infect 19:E458–E465
9. McAuliffe GN, Anderson TP, Stevens M, Adams J, Coleman R, Mahagamasekera P et al (2013) Systematic application of multiplex PCR enhances the detection of bacteria, parasites, and viruses in stool samples. J Infect 67(2):122–129
10. Coupland LJ, McElarney I, Meader E, Cowley K, Alcock L, Naunton J et al (2013) Simultaneous detection of viral and bacterial enteric pathogens using the Seeplex® Diarrhea ACE detection system. Epidemiol Infect 141(10):2111–2121
11. Koziel M, Kiely R, Blake L, O’Callaghan I, Corcoran GD, Lucey B et al (2013) Improved detection of bacterial pathogens in patients presenting with gastroenteritis by use of the EntericBio real-time Gastro Panel I assay. J Clin Microbiol 51:2679–2685
12. Rohner P, Pittet D, Pepey B, Nije-Kinge T, Auckenthaler R (1997) Etiological agents of infectious diarrhea: implications for requests for microbial culture. J Clin Microbiol 35(6):1427–1432
13. Onori M, Coltella L, Mancinelli L, Argentieri M, Menichella D, Villani A et al (2014) Evaluation of a multiplex PCR assay for simultaneous detection of bacterial and viral enteropathogens in stool samples of paediatric patients. Diagn Microbiol Infect Dis 79(2):149–154. doi:10.1016/j.diagmicrobio.2014.02.004
14. Cunningham SA, Sloan LM, Nyre LM, Vetter EA, Mandrekar J, Patel R (2010) Three-hour molecular detection of Campylobacter, Salmonella, Yersinia, and Shigella species in feces with accuracy as high as that of culture. J Clin Microbiol 48(8):2929–2933
15. Leblanc-Maridor M, Beaudeau F, Seegers H, Denis M, Belloc C (2011) Rapid identification and quantification of Campylobacter coli and Campylobacter jejuni by real-time PCR in pure cultures and in complex samples. BMC Microbiol 11:113
16. Seyler L, Lalvani A, Collins L, Goddard L, Bowler IC (2007) Safety and cost savings of an improved three-day rule for stool culture in hospitalised children and adults. J Hosp Infect 67(2):121–126
17. Wood M (2001) When stool cultures from adult inpatients are appropriate. Lancet 357(9260):901–902
18. Morris AJ, Murray PR, Reller LB (1996) Contemporary testing for enteric pathogens: the potential for cost, time, and health care savings. J Clin Microbiol 34(7):1776–1778