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Volumn 49, Issue 11, 2014, Pages 1161-1168

A rapid nile red fluorescence-based method for triacylglycerol content in microspore-derived cell suspension cultures of Brassica napus

Author keywords

Lipid analysis; Lipid biosynthesis; Neutral lipid content; Nile red; Oilseed rape

Indexed keywords

TRIACYLGLYCEROL; FLUORESCENT DYE; NILE RED; OXAZINE DERIVATIVE;

EID: 84910646215     PISSN: 00244201     EISSN: 15589307     Source Type: Journal    
DOI: 10.1007/s11745-014-3952-1     Document Type: Article
Times cited : (8)

References (38)
  • 1
    • 84856070934 scopus 로고    scopus 로고
    • Compartmentation of triacylglycerol accumulation in plants
    • 22090025 1:CAS:528:DC%2BC38XpslKrug%3D%3D 3268389
    • Chapman KD, Ohlrogge JB (2012) Compartmentation of triacylglycerol accumulation in plants. J Biol Chem 287:2288-2294
    • (2012) J Biol Chem , vol.287 , pp. 2288-2294
    • Chapman, K.D.1    Ohlrogge, J.B.2
  • 2
    • 84878975935 scopus 로고    scopus 로고
    • Biochemical pathways in seed oil synthesis
    • 23529069 1:CAS:528:DC%2BC3sXksFOlsLo%3D
    • Bates PD, Stymne S, Ohlrogge J (2013) Biochemical pathways in seed oil synthesis. Curr Opin Plant Biol 16:358-364
    • (2013) Curr Opin Plant Biol , vol.16 , pp. 358-364
    • Bates, P.D.1    Stymne, S.2    Ohlrogge, J.3
  • 3
    • 0032730777 scopus 로고    scopus 로고
    • The study of storage lipid biosynthesis using microspore-derived cultures of oil seed rape
    • 10793890 1:CAS:528:DC%2BD3cXjslKmsw%3D%3D
    • Weselake RJ, Taylor DC (1999) The study of storage lipid biosynthesis using microspore-derived cultures of oil seed rape. Prog Lipid Res 38:401-460
    • (1999) Prog Lipid Res , vol.38 , pp. 401-460
    • Weselake, R.J.1    Taylor, D.C.2
  • 4
    • 0034490775 scopus 로고    scopus 로고
    • Lipid biosynthesis in cultures of oilseed rape
    • 1:CAS:528:DC%2BD3MXhtVartLo%3D
    • Weselake RJ (2000) Lipid biosynthesis in cultures of oilseed rape. In Vitro Cell Dev Biol Plant 36:338-348
    • (2000) In Vitro Cell Dev Biol Plant , vol.36 , pp. 338-348
    • Weselake, R.J.1
  • 5
    • 0032472845 scopus 로고    scopus 로고
    • Identification of N-ethylmaleimide-sensitive and -insensitive phosphatidate phosphatase activity in microspore-derived cultures of oilseed rape
    • 1:CAS:528:DyaK1cXhslegt7s%3D
    • Furukawa-Stoffer TL, Byers SD, Hodges DM, Laroche A, Weselake RJ (1998) Identification of N-ethylmaleimide-sensitive and -insensitive phosphatidate phosphatase activity in microspore-derived cultures of oilseed rape. Plant Sci 131:139-147
    • (1998) Plant Sci , vol.131 , pp. 139-147
    • Furukawa-Stoffer, T.L.1    Byers, S.D.2    Hodges, D.M.3    Laroche, A.4    Weselake, R.J.5
  • 7
    • 0032763186 scopus 로고    scopus 로고
    • Factors enhancing diacylglycerol acyltransferase activity in microsomes of cell suspension cultures of oilseed rape
    • 10606036 1:CAS:528:DyaK1MXnvFKmsLo%3D
    • Byers SD, Laroche A, Smith KC, Weselake RJ (1999) Factors enhancing diacylglycerol acyltransferase activity in microsomes of cell suspension cultures of oilseed rape. Lipids 34:1143-1149
    • (1999) Lipids , vol.34 , pp. 1143-1149
    • Byers, S.D.1    Laroche, A.2    Smith, K.C.3    Weselake, R.J.4
  • 8
    • 0037186538 scopus 로고    scopus 로고
    • Characterization of cDNAs encoding diacylglycerol acyltransferase from cultures of Brassica napus and sucrose-mediated induction of enzyme biosynthesis
    • 11880235 1:CAS:528:DC%2BD38Xjt1Khurk%3D
    • Nykiforuk CL, Furukawa-Stoffer TL, Huff PW, Sarna M, Laroche M, Moloney MM, Weselake RJ (2002) Characterization of cDNAs encoding diacylglycerol acyltransferase from cultures of Brassica napus and sucrose-mediated induction of enzyme biosynthesis. Biochim Biophys Acta 1580:95-109
    • (2002) Biochim Biophys Acta , vol.1580 , pp. 95-109
    • Nykiforuk, C.L.1    Furukawa-Stoffer, T.L.2    Huff, P.W.3    Sarna, M.4    Laroche, M.5    Moloney, M.M.6    Weselake, R.J.7
  • 9
    • 0036753091 scopus 로고    scopus 로고
    • Sucrose-induced changes in the transcriptome of cell suspension cultures of oilseed rape reveal genes associated with lipid biosynthesis
    • 1:CAS:528:DC%2BD38Xnt1GgtLw%3D
    • Davoren JD, Nykiforuk CL, Laroche A, Weselake RJ (2002) Sucrose-induced changes in the transcriptome of cell suspension cultures of oilseed rape reveal genes associated with lipid biosynthesis. Plant Physiol Biochem 40:719-725
    • (2002) Plant Physiol Biochem , vol.40 , pp. 719-725
    • Davoren, J.D.1    Nykiforuk, C.L.2    Laroche, A.3    Weselake, R.J.4
  • 10
    • 0042624718 scopus 로고    scopus 로고
    • Properties of lysophosphatidylcholine acyltransferase from Brassica napus cultures
    • 12934675 1:CAS:528:DC%2BD3sXmt1SjtL0%3D
    • Furukawa-Stoffer T, Boyle RM, Thomson AL, Sarna MA, Weselake RJ (2003) Properties of lysophosphatidylcholine acyltransferase from Brassica napus cultures. Lipids 38:651-656
    • (2003) Lipids , vol.38 , pp. 651-656
    • Furukawa-Stoffer, T.1    Boyle, R.M.2    Thomson, A.L.3    Sarna, M.A.4    Weselake, R.J.5
  • 11
    • 84862585031 scopus 로고    scopus 로고
    • Feedback regulation of plastidic acetyl-CoA carboxylase by 18:1-acyl carrier protein in Brassica napus
    • 22665812 1:CAS:528:DC%2BC38XhtVOitLfK 3382543
    • Andre C, Haslam RP, Shanklin J (2012) Feedback regulation of plastidic acetyl-CoA carboxylase by 18:1-acyl carrier protein in Brassica napus. Proc Natl Acad Sci USA 109:10107-10112
    • (2012) Proc Natl Acad Sci USA , vol.109 , pp. 10107-10112
    • Andre, C.1    Haslam, R.P.2    Shanklin, J.3
  • 12
    • 0033942050 scopus 로고    scopus 로고
    • Purification and characterization of phosphoenolpyruvate carboxylase from Brassica napus (rapeseed) suspension cell cultures. Implications for phosphoenolpyruvate carboxylase regulation during phosphate starvation, and the integration of glycolysis with nitrogen assimilation
    • 10880970 1:CAS:528:DC%2BD3cXlt1Gnu7Y%3D
    • Moraes TF, Plaxton WC (2000) Purification and characterization of phosphoenolpyruvate carboxylase from Brassica napus (rapeseed) suspension cell cultures. Implications for phosphoenolpyruvate carboxylase regulation during phosphate starvation, and the integration of glycolysis with nitrogen assimilation. Eur J Biochem 267:4465-4476
    • (2000) Eur J Biochem , vol.267 , pp. 4465-4476
    • Moraes, T.F.1    Plaxton, W.C.2
  • 13
    • 0033942009 scopus 로고    scopus 로고
    • Purification and characterization of cytosolic pyruvate kinase from Brassica napus (rapeseed) suspension cell cultures. Implications for the integration of glycolysis with nitrogen assimilation
    • 10880971 1:CAS:528:DC%2BD3cXlt1Gnu7c%3D
    • Smith CR, Knowles VL, Plaxton WC (2000) Purification and characterization of cytosolic pyruvate kinase from Brassica napus (rapeseed) suspension cell cultures. Implications for the integration of glycolysis with nitrogen assimilation. Eur J Biochem 267:4477-4485
    • (2000) Eur J Biochem , vol.267 , pp. 4477-4485
    • Smith, C.R.1    Knowles, V.L.2    Plaxton, W.C.3
  • 14
    • 84981607316 scopus 로고
    • Induction of freezing tolerance in an embryogenic cell suspension culture of Brassica napus by abscisic acid at room temperature
    • 1:CAS:528:DyaL2sXhtFGit74%3D
    • Orr W, Keller WA, Singh J (1986) Induction of freezing tolerance in an embryogenic cell suspension culture of Brassica napus by abscisic acid at room temperature. J Plant Physiol 126:23-32
    • (1986) J Plant Physiol , vol.126 , pp. 23-32
    • Orr, W.1    Keller, W.A.2    Singh, J.3
  • 15
    • 0001352057 scopus 로고
    • Alteration of gene expression during the induction of freezing tolerance in Brassica napus suspension cultures
    • 16665763 1:CAS:528:DyaL1cXlt12qtA%3D%3D 1054325
    • Johnson-Flanagan AM, Singh J (1987) Alteration of gene expression during the induction of freezing tolerance in Brassica napus suspension cultures. Plant Physiol 85:699-705
    • (1987) Plant Physiol , vol.85 , pp. 699-705
    • Johnson-Flanagan, A.M.1    Singh, J.2
  • 16
    • 0001130444 scopus 로고
    • Role of abscisic acid in the induction of freezing tolerance in Brassica napus suspension-cultured cells
    • 16668089 1:CAS:528:DyaK3MXktVGhu7o%3D 1077649
    • Johnson-Flanagan AM, Huiwen Z, Thiagarajah MR, Saini HS (1991) Role of abscisic acid in the induction of freezing tolerance in Brassica napus suspension-cultured cells. Plant Physiol 95:1044-1048
    • (1991) Plant Physiol , vol.95 , pp. 1044-1048
    • Johnson-Flanagan, A.M.1    Huiwen, Z.2    Thiagarajah, M.R.3    Saini, H.S.4
  • 17
    • 0031238682 scopus 로고    scopus 로고
    • Disruption of the phosphate-starvation response of oilseed rape suspension cells by the fungicide phosphonate
    • 9299791 1:CAS:528:DyaK2sXlvVChuro%3D
    • Carswell MC, Grant BR, Plaxton WC (1997) Disruption of the phosphate-starvation response of oilseed rape suspension cells by the fungicide phosphonate. Planta 203:67-74
    • (1997) Planta , vol.203 , pp. 67-74
    • Carswell, M.C.1    Grant, B.R.2    Plaxton, W.C.3
  • 18
    • 0034624221 scopus 로고    scopus 로고
    • Upregulation of vacuolar H+-translocating pyrophosphatase by phosphate starvation of Brassica napus (rapeseed) suspension cell cultures
    • 11113457 1:CAS:528:DC%2BD3cXoslGhs78%3D
    • Palma DA, Blumwald E, Plaxton WC (2000) Upregulation of vacuolar H+-translocating pyrophosphatase by phosphate starvation of Brassica napus (rapeseed) suspension cell cultures. FEBS Lett 486:155-158
    • (2000) FEBS Lett , vol.486 , pp. 155-158
    • Palma, D.A.1    Blumwald, E.2    Plaxton, W.C.3
  • 19
    • 1642546961 scopus 로고    scopus 로고
    • Phosphite accelerates programmed cell death in phosphate-starved oilseed rape (Brassica napus) suspension cell cultures
    • 12920596 1:CAS:528:DC%2BD3sXptleltbw%3D
    • Singh VK, Wood SM, Knowles VL, Plaxton WC (2003) Phosphite accelerates programmed cell death in phosphate-starved oilseed rape (Brassica napus) suspension cell cultures. Planta 218:233-239
    • (2003) Planta , vol.218 , pp. 233-239
    • Singh, V.K.1    Wood, S.M.2    Knowles, V.L.3    Plaxton, W.C.4
  • 21
    • 0001156407 scopus 로고
    • High plating efficiency and plant regeneration frequency in low density protoplast cultures derived from an embryogenic Brassica napus cell suspension
    • Simmonds DH, Long NE, Keller WA (1991) High plating efficiency and plant regeneration frequency in low density protoplast cultures derived from an embryogenic Brassica napus cell suspension. Plant Cell Tiss Organ Cult 27:231-241
    • (1991) Plant Cell Tiss Organ Cult , vol.27 , pp. 231-241
    • Simmonds, D.H.1    Long, N.E.2    Keller, W.A.3
  • 23
    • 0023270025 scopus 로고
    • Use of Nile red for the rapid in situ quantitation of lipids on thin-layer chromatograms
    • 3681147 1:CAS:528:DyaL2sXmtlantr0%3D
    • Fowler SD, Brown WJ, Warfel J, Greenspan P (1987) Use of Nile red for the rapid in situ quantitation of lipids on thin-layer chromatograms. J Lipid Res 28:1225-1232
    • (1987) J Lipid Res , vol.28 , pp. 1225-1232
    • Fowler, S.D.1    Brown, W.J.2    Warfel, J.3    Greenspan, P.4
  • 24
    • 0141643387 scopus 로고    scopus 로고
    • Rapid screening method for quantitation of bacterial cell lipids from whole cells
    • 1:CAS:528:DC%2BD3sXns12ls70%3D
    • Izard J, Limberger RJ (2003) Rapid screening method for quantitation of bacterial cell lipids from whole cells. J Microbiol Meth 55:411-418
    • (2003) J Microbiol Meth , vol.55 , pp. 411-418
    • Izard, J.1    Limberger, R.J.2
  • 25
    • 0022360487 scopus 로고
    • A rapid screening method for lipid-accumulating yeast using a replica-printing technique
    • Christopher TE, Ratledge C, Gilbert SC (1983) A rapid screening method for lipid-accumulating yeast using a replica-printing technique. J Microbiol Meth 4:203-210
    • (1983) J Microbiol Meth , vol.4 , pp. 203-210
    • Christopher, T.E.1    Ratledge, C.2    Gilbert, S.C.3
  • 26
    • 1142273243 scopus 로고    scopus 로고
    • Rapid estimation of lipids in oleaginous fungi and yeasts using Nile red fluorescence
    • 14967224 1:CAS:528:DC%2BD2cXht1ykurw%3D
    • Kimura K, Yamaoka M, Kamisaka Y (2004) Rapid estimation of lipids in oleaginous fungi and yeasts using Nile red fluorescence. J Microbiol Methods 56:331-338
    • (2004) J Microbiol Methods , vol.56 , pp. 331-338
    • Kimura, K.1    Yamaoka, M.2    Kamisaka, Y.3
  • 27
    • 70350247593 scopus 로고    scopus 로고
    • Simple methods to detect triacylglycerol biosynthesis in a yeast-based recombinant system
    • 19763656 1:CAS:528:DC%2BD1MXht1OgtLvK
    • Siloto RMP, Truksa M, He X, McKeon T, Weselake RJ (2009) Simple methods to detect triacylglycerol biosynthesis in a yeast-based recombinant system. Lipids 44:963-973
    • (2009) Lipids , vol.44 , pp. 963-973
    • Siloto, R.M.P.1    Truksa, M.2    He, X.3    McKeon, T.4    Weselake, R.J.5
  • 28
    • 0030808359 scopus 로고    scopus 로고
    • Characterization of the growth and lipid content of the diatom Chaetoceros muelleri
    • 1:CAS:528:DyaK2sXlsVKkt7o%3D
    • McGinnis KM, Dempster TA, Sommerfeld MR (1997) Characterization of the growth and lipid content of the diatom Chaetoceros muelleri. J Appl Phycol 9:19-24
    • (1997) J Appl Phycol , vol.9 , pp. 19-24
    • McGinnis, K.M.1    Dempster, T.A.2    Sommerfeld, M.R.3
  • 29
    • 26844522529 scopus 로고    scopus 로고
    • Production and cellular localization of neutral long-chain lipids in the haptophyte algae Isochrysis galbana and Emiliania huxleyi
    • 1:CAS:528:DC%2BD2MXhtF2nsb3O
    • Eltgroth ML, Watwood RL, Wolfe GV (2005) Production and cellular localization of neutral long-chain lipids in the haptophyte algae Isochrysis galbana and Emiliania huxleyi. J Phycol 41:1000-1009
    • (2005) J Phycol , vol.41 , pp. 1000-1009
    • Eltgroth, M.L.1    Watwood, R.L.2    Wolfe, G.V.3
  • 30
    • 33847292769 scopus 로고    scopus 로고
    • Fluorescent measurement of microalgal neutral lipids
    • 1:CAS:528:DC%2BD2sXhvVCrt7w%3D
    • Elsey D, Jameson D, Raleigh B, Cooney MJ (2007) Fluorescent measurement of microalgal neutral lipids. J Microbiol Meth 68:639-642
    • (2007) J Microbiol Meth , vol.68 , pp. 639-642
    • Elsey, D.1    Jameson, D.2    Raleigh, B.3    Cooney, M.J.4
  • 31
    • 41849116000 scopus 로고    scopus 로고
    • Effect of iron on growth and lipid accumulation in Chlorella vulgaris
    • 17993270 1:CAS:528:DC%2BD1cXks1Crtbg%3D
    • Liu Z, Wang G, Zhou B (2008) Effect of iron on growth and lipid accumulation in Chlorella vulgaris. Bioresour Technol 99:4717-4722
    • (2008) Bioresour Technol , vol.99 , pp. 4717-4722
    • Liu, Z.1    Wang, G.2    Zhou, B.3
  • 32
    • 62349095514 scopus 로고    scopus 로고
    • A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae
    • Chen W, Zhang C, Song L, Sommerfeld M, Hu Q (2009) A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae. J Microbiol Meth 1:41-47
    • (2009) J Microbiol Meth , vol.1 , pp. 41-47
    • Chen, W.1    Zhang, C.2    Song, L.3    Sommerfeld, M.4    Hu, Q.5
  • 33
    • 13544265408 scopus 로고    scopus 로고
    • The use of a fluorescent dye, Nile red, to evaluate the lipid content of single mammalian oocytes
    • 15710202 1:CAS:528:DC%2BD2MXhtlSltb8%3D
    • Genicot G, Leroy J, Van Soom A, Donnay I (2005) The use of a fluorescent dye, Nile red, to evaluate the lipid content of single mammalian oocytes. Theriogenology 63:1181-1194
    • (2005) Theriogenology , vol.63 , pp. 1181-1194
    • Genicot, G.1    Leroy, J.2    Van Soom, A.3    Donnay, I.4
  • 34
    • 77957334598 scopus 로고    scopus 로고
    • Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae
    • 20638272 1:CAS:528:DC%2BC3cXht1CgsLvO
    • Chen W, Sommerfeld M, Hu Q (2011) Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae. Bioresour Technol 102:135-141
    • (2011) Bioresour Technol , vol.102 , pp. 135-141
    • Chen, W.1    Sommerfeld, M.2    Hu, Q.3
  • 35
    • 0028155257 scopus 로고
    • Optimal staining of lipids in bivalve larvae with Nile Red
    • Castell LL, Mann R (1994) Optimal staining of lipids in bivalve larvae with Nile Red. Aquaculture 1:89-100
    • (1994) Aquaculture , vol.1 , pp. 89-100
    • Castell, L.L.1    Mann, R.2
  • 36
    • 0018128226 scopus 로고
    • Lipid extraction of tissues with a low-toxicity solvent
    • 727482 1:CAS:528:DyaE1cXmtFWmsL0%3D
    • Hara A, Radin NS (1978) Lipid extraction of tissues with a low-toxicity solvent. Anal Biochem 90:420-426
    • (1978) Anal Biochem , vol.90 , pp. 420-426
    • Hara, A.1    Radin, N.S.2
  • 37
    • 0022608640 scopus 로고
    • Fatty acid composition of leaf lipids determined after combined digestion and fatty acid methyl ester formation from fresh tissue
    • 3954036 1:CAS:528:DyaL28XkvVWitg%3D%3D
    • Browse J, McCourtt PJ, Sommerville CR (1986) Fatty acid composition of leaf lipids determined after combined digestion and fatty acid methyl ester formation from fresh tissue. Anal Biochem 152:141-145
    • (1986) Anal Biochem , vol.152 , pp. 141-145
    • Browse, J.1    McCourtt, P.J.2    Sommerville, C.R.3
  • 38
    • 84910618150 scopus 로고    scopus 로고
    • Storage product metabolism in microspore-derived cultures of Brassicaceae
    • W. Keller K. Kasha (eds) Haploids in crop improvement II. Springer Berlin
    • Weselake R (2005) Storage product metabolism in microspore-derived cultures of Brassicaceae. In: Keller W, Kasha K (eds) Don Palmer CE. Haploids in crop improvement II. Springer, Berlin
    • (2005) Don Palmer CE
    • Weselake, R.1


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