Trypanosoma cruzi diversity in the Gran Chaco: Mixed infections and differential host distribution of TcV and TcVI

Infection, Genetics and Evolution

Volumn 29, Issue , 2015, Pages 53-59

  Monje Rumi, María M ^a   Brandán, Cecilia Pérez ^a   Ragone, Paula G ^a   Tomasini, Nicolás ^a   Lauthier, Juan J ^a   Alberti D'Amato, Anahí M ^a   Cimino, Rubén O ^a   Orellana, Viviana ^a   Basombrío, Miguel A ^a   Diosque, Patricio ^a  

^a UNIVERSIDAD NACIONAL DE SALTA   (Argentina)

Author keywords

Argentina; Domestic cycle; Hybridization; PCR; Prevalence

Indexed keywords

CANIS FAMILIARIS; TRYPANOSOMA CRUZI;

DNA, PROTOZOAN;

ADOLESCENT; ADULT; ANIMALS; ARGENTINA; BLOOD; CHAGAS DISEASE; CHILD; CLASSIFICATION; COINFECTION; CROSS-SECTIONAL STUDIES; DOG DISEASES; DOGS; GENETICS; GENOTYPE; HUMANS; ISOLATION AND PURIFICATION; MALE; MIDDLE AGED; PARASITOLOGY; RURAL POPULATION; TRYPANOSOMA CRUZI; VETERINARY; YOUNG ADULT;

ADOLESCENT; ADULT; ANIMALS; ARGENTINA; CHAGAS DISEASE; CHILD; COINFECTION; CROSS-SECTIONAL STUDIES; DNA, PROTOZOAN; DOG DISEASES; DOGS; GENOTYPE; HUMANS; MALE; MIDDLE AGED; RURAL POPULATION; TRYPANOSOMA CRUZI; YOUNG ADULT;

EID: 84910638620     PISSN: 15671348     EISSN: 15677257     Source Type: Journal    
DOI: 10.1016/j.meegid.2014.11.001     Document Type: Article

References (29)

1. Brenière S.F., Bosseno M.F., Telleria J., Bastrenta B., Yaksic N., Noireau F., Alcazar J.L., Barnabé C., Wincker P., Tibayrenc M. Different behaviour of two Trypanosoma cruzi major clones: transmission and circulation in Bolivian young patients. Exp. Parasitol. 1998, 89:285-295.
2. Bosseno M.F., Espinoza B., Sanchez B., Breniere S.F. Mexican Trypanosoma cruzi stocks: analysis of minicircle kDNA homologies by cross-hybridization. Mem. Inst. Oswaldo Cruz 2000, 95:473-476.
3. Burgos J.M., Altcheh J., Bisio M., Duffy T., Valadares H.M., Seidenstein M.E., Piccinali R., Freitas J.M., Levin M.J., Macchi L., Macedo A.M., Freilij H., Schijman A.G. Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease. Int. J. Parasitol. 2007, 37:1319-1327.
4. Brenière S.F., Bosseno M.F., Noireau F., Yacsik N., Liegeard P., Aznar C., Hontebeyrie M. Integrate study of a Bolivian population infected by Trypanosoma cruzi, the agent of Chagas disease. Mem. Inst. Oswaldo Cruz 2002, 97:289-295.
5. Britto C., Cardoso M.A., Vanni C.M., Hasslocher-Moreno A., Xavier S.S., Oelemann W., Santoro A., Pirmez C., Morel C.M., Wincker P. Polymerase chain reaction detection of Trypanosoma cruzi in human blood samples as a tool for diagnosis and treatment evaluation. Parasitology 1995, 110:241-247.
6. Cardinal M.V., Lauricella M.A., Ceballos L.A., Lanati L., Marcet P.L., Levin M.J., Kitron U., Gurtler R.E., Schijman A.G. Molecular epidemiology of domestic and sylvatic Trypanosoma cruzi infection in rural northwestern Argentina. Int. J. Parasitol. 2008, 38:1533-1543.
7. Cimino R.O., Rumi M.M., Ragone P., Lauthier J., D'Amato A.A., Quiroga I.R., Gil J.F., Cajal S.P., Acosta N., Juarez M., Krolewiecki A., Orellana V., Zacca R., Marcipar I., Diosque P., Nasser J.R. Immuno-enzymatic evaluation of the recombinant TSSA-II protein of Trypanosoma cruzi in dogs and human sera: a tool for epidemiological studies. Parasitology 2011, 138:995-1002.
8. Cura C.I., Lucero R.H., Bisio M., Oshiro E., Formichelli L.B., Burgos J.M., Lejona S., Brusés B.L., Hernández D.O., Severini G.V., Velazquez E., Duffy T., Anchart E., Lattes R., Altcheh J., Freilij H., Diez M., Nagel C., Vigliano C., Favaloro L., Favaloro R.R., Merino D.E., Sosa-Estani S., Schijman A.G. Trypanosoma cruzi discrete typing units in Chagas disease patients from endemic and non-endemic regions of Argentina. Parasitology 2012, 139:516-521.
9. Diez C., Lorenz V., Ortiz S., Gonzalez V., Racca A., Bontempi I., Manattini S., Solari A., Marcipar I. Genotyping of Trypanosoma cruzi sublineage in human samples from a North-East Argentina area by hybridization with DNA probes and specific polymerase chain reaction (PCR). Am. J. Trop. Med. Hyg. 2010, 82:67-73.
10. Di Rienzo J., Casanoves F., Balzarini M., Gonzales L., Tablada M., Robledo O. InfoStat versión 2009 2009, Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina.
11. Diosque P., Barnabe C., Padilla A.M., Marco J.D., Cardozo R.M., Cimino R.O., Nasser J.R., Tibayrenc M., Basombrio M.A. Multilocus enzyme electrophoresis analysis of Trypanosoma cruzi isolates from a geographically restricted endemic area for Chagas' disease in Argentina. Int. J. Parasitol. 2003, 33:997-1003.
12. Diosque P., Padilla A.M., Cimino R.O., Cardozo R.M., Negrette O.S., Marco J.D., Zacca R., Meza C., Juarez A., Rojo H., Rey R., Corrales R.M., Nasser J.R., Basombrio M.A. Chagas disease in rural areas of Chaco Province, Argentina: epidemiologic survey in humans, reservoirs, and vectors. Am. J. Trop. Med. Hyg. 2004, 71:590-593.
13. Enriquez G.F., Cardinal M.V., Orozco M.M., Lanati L., Schijman A.G., Gürtler R.E. Discrete typing units of Trypanosoma cruzi identified in rural dogs and cats in the humid Argentinean Chaco. Parasitology 2013, 140:303-308.
14. Faul F., Erdfelder E., Lang A.-G., Buchner A. G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav. Res. Methods 2007, 39:175-191.
15. Gürtler R.E. Sustainability of vector control strategies in the Gran Chaco Region: current challenges and possible approaches. Mem. Inst. Oswaldo Cruz 2009, 104(Suppl. 1):52-59.
16. Lauthier J.J., Tomasini N., Barnabe C., Rumi M.M., D'Amato A.M., Ragone P.G., Yeo M., Lewis M.D., Llewellyn M.S., Basombrio M.A., Miles M.A., Tibayrenc M., Diosque P. Candidate targets for multilocus sequence typing of Trypanosoma cruzi: validation using parasite stocks from the Chaco Region and a set of reference strains. Infect. Genet. Evol. 2012, 12:350-358.
17. Llewellyn M.S., Lewis M.D., Acosta N., Yeo M., Carrasco H.J., et al. Trypanosoma cruzi IIc: phylogenetic and phylogeographic insights from sequence and microsatellite analysis and potential impact on emergent chagas disease. PLoS Negl. Trop. Dis. 2009, 3:e510.
18. Macedo A.M., Machado C.R., Oliveira R.P., Pena S.D.J. Trypanosoma cruzi: genetic structure of populations and relevance of genetic variability to the pathogenesis of Chagas Disease. Mem. Inst. Oswaldo Cruz 2004, 99:1-12.
19. Maffey L., Cardinal M.V., Ordonez-Krasnowski P.C., Lanati L.A., Lauricella M.A., Schijman A.G., Gurtler R.E. Direct molecular identification of Trypanosoma cruzi discrete typing units in domestic and peridomestic Triatoma infestans and Triatomasordida from the Argentine Chaco. Parasitology 2012, 139:1570-1579.
20. Miles M.A., Llewellyn M.S., Lewis M.D., Yeo M., Baleela R., Fitzpatrick S., Gaunt M.W., Mauricio I.L. The molecular epidemiology and phylogeography of Trypanosoma cruzi and parallel research on Leishmania: looking back and to the future. Parasitology 2009, 136:1509-1528.
21. Moreno M.L., Hoyos L., Cabido M., Catala S.S., Gorla D.E. Exploring the association between Trypanosoma cruzi infection in rural communities and environmental changes in the southern Gran Chaco. Mem. Inst. Oswaldo Cruz 2012, 107:231-237.
22. Perez E., Monje M., Chang B., Buitrago R., Parrado R., Barnabe C., Noireau F., Breniere S.F. Predominance of hybrid discrete typing units of Trypanosoma cruzi in domestic Triatoma infestans from the Bolivian Gran Chaco region. Infect. Genet. Evol. 2013, 13:116-123.
23. Ragone P.G., Perez Brandan C., Padilla A.M., Monje Rumi M., Lauthier J.J., Alberti D'Amato A.M., Tomasini N., Cimino R.O., Romero N.M., Portelli M., Nasser J.R., Basombrio M.A., Diosque P. Biological behavior of different Trypanosoma cruzi isolates circulating in an endemic area for Chagas disease in the Gran Chaco region of Argentina. Acta Trop. 2012, 123:196-201.
24. Rumi M.M., Perez Brandan C., Gil J.F., D'Amato A.M., Ragone P.G., Lauthier J.J., Tomasini N., Cimino R.O., Orellana V., Lacunza C.D., Nasser J.R., Basombrio M.A., Diosque P. Benznidazole treatment in chronic children infected with Trypanosoma cruzi: serological and molecular follow-up of patients and identification of Discrete Typing Units. Acta Trop. 2013, 128:130-136.
25. Tomasini N., Lauthier J.J., Monje Rumi M.M., Ragone P.G., Alberti D'Amato A.A., Perez Brandan C., Cura C.I., Schijman A.G., Barnabe C., Tibayrenc M., Basombrio M.A., Falla A., Herrera C., Guhl F., Diosque P. Interest and limitations of spliced leader intergenic region sequences for analyzing Trypanosoma cruzi I phylogenetic diversity in the Argentinean Chaco. Infect. Genet. Evol. 2011, 11:300-307.
26. Veas F., Breniere S.F., Cuny G., Brengues C., Solari A., Tibayrenc M. General procedure to construct highly specific kDNA probes for clones of Trypanosoma cruzi for sensitive detection by polymerase chain reaction. Cell Mol. Biol. 1991, 37:73-84.
27. WHO, 2014. World Health Organization (WHO). Chagas Disease (American trypanosomiasis). Fact sheet N°340. Update March 2014. <>. http://www.who.int/mediacenter/factsheets/fs340/en/.
28. Zingales B., Andrade S.G., Briones M.R., Campbell D.A., Chiari E., Fernandes O., Guhl F., Lages-Silva E., Macedo A.M., Machado C.R., Miles M.A., Romanha A.J., Sturm N.R., Tibayrenc M., Schijman A.G. A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI. Mem. Inst. Oswaldo Cruz 2009, 104:1051-1054.
29. Zingales B., Miles M.A., Campbell D.A., Tibayrenc M., Macedo A.M., Teixeira M.M., Schijman A.G., Llewellyn M.S., Lages-Silva E., Machado C.R., Andrade S.G., Sturm N.R. The revised Trypanosoma cruzi subspecific nomenclature: rationale, epidemiological relevance and research applications. Infect. Genet. Evol. 2012, 12:240-253.