Proliferation assay amplification by IL-2 in model primary and recall antigen systems

BMC Research Notes

Volumn 7, Issue 1, 2014, Pages

  Kennell, Amy Sm ^a   Gould, Keith G ^a   Salaman, Myer R ^a  

^a IMPERIAL COLLEGE SCHOOL OF MEDICINE   (United Kingdom)

Author keywords

IL 2; Keyhole limpet haemocyanin; Pokeweed mitogen; Primary response; T lymphocyte proliferation

Indexed keywords

ANTIGEN; INTERLEUKIN 2;

CELL PROLIFERATION; HUMAN; IMMUNOLOGICAL MEMORY; IMMUNOLOGY; PHYSIOLOGY;

ANTIGENS; CELL PROLIFERATION; HUMANS; IMMUNOLOGIC MEMORY; INTERLEUKIN-2;

EID: 84910633023     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-7-662     Document Type: Article

References (17)

1. The IL-2 receptor complex: its structure, function, and target genes. Minami Y, Kono T, Miyazaki T, Taniguchi T, Annu Rev Immunol 1993 11 245 268 10.1146/annurev.iy.11.040193.001333 8476561
2. T cell lines and hybrids in mouse and man. Fathman CG, Engleman EG, Handbook of experimental immunology Oxford: Blackwell Scientific, Weir DM, Herzenberg LA, Blackwell C, Herzenberg LA, 4 1993 69.1 69.12
3. GAD65-reactive T cells are activated in patients with autoimmune type 1a diabetes. Viglietta V, Kent SC, Orban T, Hafler DA, J Clin Invest 2002 109 895 903 10.1172/JCI0214114 11927616
4. A culture amplified multi-parametric intracellular cytokine assay (CAMP-ICC) for enhanced detection of antigen specific T-cell responses. Munier CM, Zaunders JJ, Ip S, Cooper DA, Kelleher AD, J Immunol Methods 2009 345 1 16 10.1016/j.jim.2009.03.013 19345223
5. The human primary immune response to keyhole limpet haemocyanin: interrelationships of delayed hypersensitivity, antibody response and in vitro blast transformation. Curtis JE, Hersh EM, Harris JE, McBride C, Freireich EJ, Clin Exp Immunol 1970 6 473 491 4320164
6. In vitro primary responses of human T cells to soluble protein antigens. Plebanski M, Burtles SS, J Immunol Methods 1994 170 15 25 10.1016/0022-1759(94)90241-0 7512606
7. IL 2 alone is mitogenic only for Tac-positive lymphocytes in human peripheral blood. Taylor DS, Kern JA, Nowell PC, J Immunol 1986 136 1620 1624 3005396
8. Improvement of the in vitro T cell proliferation assay by a modified method that separates the antigen recognition and IL-2-dependent steps. Chain B, McCafferty I, Wallace G, Askenase PW, J Immunol Methods 1987 99 221 228 10.1016/0022-1759(87)90131-1 3108408
9. Role of B70/B7-2 in CD4+ T-cell immune responses induced by dendritic cells. Fagnoni FF, Takamizawa M, Godfrey WR, Rivas A, Azuma M, Okumura K, Engelman EG, Immunology 1995 85 467 474 7558137
10. B7-1 and B7-2: similar costimulatory ligands with different biochemical, oligomeric and signaling properties. Bhatia S, Edidin M, Almo SC, Nathenson SG, Immunol Lett 2006 104 70 75 10.1016/j.imlet.2005.11.019 16413062
11. Stimulation of human T lymphocytes by LPS is MHC unrestricted, but strongly dependent on B7 interactions. Mattern T, Flad HD, Brade L, Rietschel ET, Ulmer AJ, J Immunol 1998 160 3412 3418 9531301
12. Expression and function of Toll-like receptors in T lymphocytes. Kabelitz D, Curr Opin Immunol 2007 19 39 45 10.1016/j.coi.2006.11.007 17129718
13. The T cell dependence of B cell differentiation induced by pokeweed mitogen. Keightley RG, Cooper MD, Lawton AR, J Immunol 1976 117 1538 1544 794413
14. Analysis of the mechanisms of T cell-dependent polyclonal activation of human B cells. Induction of human B cell responses by fixed activated T cells. Tohma S, Lipsky PE, J Immunol 1991 146 2544 2552 1707911
15. Cytokine production in cell culture by peripheral blood mononuclear cells from immunocompetent hosts. Katial RK, Sachanandani D, Pinney C, Lieberman MM, Clin Diagn Lab Immunol 1998 5 78 81 9455885
16. Complex cytokine profiles induced by BCG vaccination in UK infants. Lalor MK, Smith SG, Floyd S, Gorak-Stolinska P, Weir RE, Blitz R, Branson K, Fine PE, Dockrell HM, Vaccine 2010 28 1635 1641 10.1016/j.vaccine.2009.11.004 19941997
17. A two-step hypothesis for the appearance of autoimmune disease. Salaman MR, Autoimmunity 2003 36 57 61 10.1080/0891693031000068637 12820686