Production and use of lentivirus to selectively transduce primary oligodendrocyte precursor cells for in vitro myelination assays

Journal of Visualized Experiments

Volumn , Issue 95, 2015, Pages

  Peckham, Haley M ^a   Ferner, Anita H ^a   Giuffrida, Lauren ^a   Murray, Simon S ^a   Xiao, Junhua ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Cocultures; Developmental biology; Dorsal root ganglion neurons; Issue 95; Lentivirus; Myelination; Oligodendrocyte; Oligodendrocyte precursor cells

Indexed keywords

CELL CULTURE; COCULTURE; CYTOLOGY; HEK293 CELL LINE; HUMAN; LENTIVIRINAE; METABOLISM; MYELIN SHEATH; NERVE CELL; NERVOUS SYSTEM DEVELOPMENT; NEURAL STEM CELL; OLIGODENDROGLIA; PHYSIOLOGY; SIGNAL TRANSDUCTION; SPINAL GANGLION; VIROLOGY;

CELLS, CULTURED; COCULTURE TECHNIQUES; GANGLIA, SPINAL; HEK293 CELLS; HUMANS; LENTIVIRUS; MYELIN SHEATH; NEURAL STEM CELLS; NEUROGENESIS; NEURONS; OLIGODENDROGLIA; SIGNAL TRANSDUCTION;

EID: 84909993049     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52179     Document Type: Article

References (13)

1. Baumann, N, Pham-Dinh, D. Biology of oligodendrocyte and myelin in the mammalian central nervous system. Physiol Rev. 81, (2), 871-927 (2001).
2. Watkins, T. A, Emery, B, Mulinyawe, S, Barres, B. A. Distinct stages of myelination regulated by gamma-secretase and astrocytes in a rapidly myelinating CNS coculture system. Neuron. 60, (4), 555-569 (2008).
3. Lee, K, et al. MDGAs interact selectively with neuroligin-2 but not other neuroligins to regulate inhibitory synapse development. Proc Natl Acad Sci U S A. 110, (1), 336-341 (2013).
4. Xiao, J, et al. BDNF exerts contrasting effects on peripheral myelination of NGF-dependent and BDNF-dependent DRG neurons. J Neurosci. 29, (13), 4016-4022 (2009).
5. Chan, J. R, et al. NGF controls axonal receptivity to myelination by Schwann cells or oligodendrocytes.Neuron. 43, (2), 183-191 (2004).
6. Xiao, J, et al. Brain-Derived Neurotrophic Factor Promotes Central Nervous System Myelination via a Direct Effect upon Oligodendrocytes. Neurosignals. 18, (3), 186-202 (2010).
7. Lundgaard, I, et al. Neuregulin and BDNF induce a switch to NMDA receptor-dependent myelination by oligodendrocytes. PLoS Biology. 11, (12), e1001743(2013).
8. Kleitman, N, W, P. M, Bunge, R. P. Tissue culture methodes for the study of myelination. MIT Press Cambridge, MA (1991).
9. Xiao, J, et al. Extracellular signal-regulated kinase 1/2 signaling promotes oligodendrocyte myelination in vitro. J Neurochem. 122, (6), 1167-1180 (2012).
10. Wong, A. W, Xiao, J, Kemper, D, Kilpatrick, T. J, Murray, S. S. Oligodendroglial expression of TrkB independently regulates myelination and progenitor cell proliferation. The Journal of Neuroscience. 33, (11), 4947-4957 (2013).
11. Li, Z, et al. Molecular cloning, Characterization and Expression of miR-15a-3p and miR-15b-3p in Dairy Cattle. Molecular and Cellular Probes. (2014).
12. Emery, B, et al. Myelin gene regulatory factor is a critical transcriptional regulator required for CNS myelination. Cell. 138, (1), 172-185 (2009).
13. Murai, K, et al. Nuclear receptor TLX stimulates hippocampal neurogenesis and enhances learning and memory in a transgenic mouse model. Proc Natl Acad Sci U S A. 111, (25), 9115-9120 (2014).