Penicillin-resistant, ampicillin-susceptible Enterococcus faecalis of hospital origin: Pbp4 gene polymorphism and genetic diversity

Infection, Genetics and Evolution

Volumn 28, Issue , 2014, Pages 289-295

  Conceição, Natália ^a,b   da Silva, Lucas Emanuel Pinheiro ^a   da Costa Darini, Ana Lúcia ^b   Pitondo Silva, André ^b   de Oliveira, Adriana Gonçalves ^a  

^a FEDERAL UNIVERSITY OF TRIÂNGULO MINEIRO   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

E. faecalis; MLST; Penicillin binding proteins; Penicillin resistance; PFGE

Indexed keywords

AMOXICILLIN; AMPICILLIN; BETA LACTAM; BETA LACTAMASE; IMIPENEM; PENICILLIN DERIVATIVE; PIPERACILLIN; PENICILLIN BINDING PROTEIN;

AMINO ACID SUBSTITUTION; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; AUTOMATED SEQUENCER; AUTOMATION; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CONTROLLED STUDY; ENTEROCOCCUS FAECALIS; EPSILOMETER TEST; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC ASSOCIATION; GENETIC POLYMORPHISM; GENETIC VARIABILITY; HOSPITAL PATIENT; MINIMUM INHIBITORY CONCENTRATION; MULTILOCUS SEQUENCE TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; PBP4 GENE; PENICILLIN RESISTANT AMPICILLIN SUSCEPTIBLE ENTEROCOCCUS FAECALIS; PENICILLIN SUSCEPTIBLE AMPICILLIN SUSCEPTIBLE ENTEROCOCCUS FAECALIS; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; CROSS INFECTION; DRUG EFFECTS; GENETICS; GRAM POSITIVE INFECTION; HUMAN; MICROBIAL SENSITIVITY TEST; MICROBIOLOGY; PENICILLIN RESISTANCE;

AMPICILLIN; BETA-LACTAMASES; CROSS INFECTION; ELECTROPHORESIS, GEL, PULSED-FIELD; ENTEROCOCCUS FAECALIS; GENETIC VARIATION; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; MICROBIAL SENSITIVITY TESTS; MULTILOCUS SEQUENCE TYPING; PENICILLIN RESISTANCE; PENICILLIN-BINDING PROTEINS; PENICILLINS;

EID: 84909592097     PISSN: 15671348     EISSN: 15677257     Source Type: Journal    
DOI: 10.1016/j.meegid.2014.10.018     Document Type: Article

References (30)

1. Amin N.E., Lund B., Tjernlund A., Lundberg C., Jalakas K., Wretlind B. Mechanisms of resistance to imipenem in imipenem-resistant, ampicillin-sensitive Enterococcus faecium. APMIS 2001, 109:791-796.
2. Arias C.A., Contreras G.A., Murray B.E. Management of multidrug resistant enterococcal infections. Clin. Microbiol. Infect. 2010, 16:555-562.
3. Campanile F., Bartoloni A., Bartalesi F., Borbone S., Mangani V., Mantella A., Nicoletti G., Paradise F., Russo G., Strohmeyer M., Stefani S. Molecular alterations of VanA element in vancomycin-resistant enterococci isolated during a survey of colonized patients in an Italian intensive care unit. Microb. Drug Resist. 2003, 9:191-199.
4. CLSI (Clinical and Laboratory Standards Institute), 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, ninth ed. Approved Standard M7-A9. Clinical and Laboratory Standards Institute, Wayne, PA.
5. CLSI (Clinical and Laboratory Standards Institute), 2013. Performance Standards for Antimicrobial Susceptibility Testing; 23rd Informational Supplement. CLSI Document M100-S23. Clinical Laboratory Standards Institute, Wayne, PA.
6. Conceição N., Oliveira C.C.H.B., Silva P.R., Avila B.G., Oliveira A.G. Trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital: a 4-year study. Rev. Soc. Bras. Med. Trop. 2011, 44:177-181.
7. Conceição N., Oliveira C.C.H.B., Silva L.E.P., Souza L.R.C., Oliveira A.G. Ampicillin susceptibility can predict in vitro susceptibility of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates to amoxicillin but not to imipenem and piperacillin. J. Clin. Microbiol. 2012, 50:3729-3731.
8. Duez C., Zorzi W., Sapunaric F., Amoroso A., Thamm I., Covette J. The penicillin resistance of Enterococcus faecalis JH2-2R results from an overproduction of the low-affinity penicillin-binding protein PBP4 and does not involve a psr-like gene. Microbiology 2001, 147:2561-2569.
9. Dutka-Malen S., Evers S., Courvalin P. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J. Clin. Microbiol. 1995, 33:24-27.
10. Eliopoulos G.M. Antimicrobial resistance in the Enterococcus. Bacterial Resistance to Antimicrobials 2008, 255-289. CRC Press, Boca Raton, FL. second ed. R.G. Wax, K. Lewis, A.A. Salyers, H. Taber (Eds.).
11. Fontana R., Amalfitano G., Satta G. Mechanisms of resistance growth inhibition and killing by β-lactam antibiotics in enterococci. Clin. Infect. Dis. 1992, 15:486-489.
12. Gawryszewska I., Hryniewicz W., Sadowy E. Penicillin resistance in Enterococcus faecalis: molecular determinants and epidemiology. Pol. J. Microbiol. 2012, 61(3):153-160.
13. Guardabassi L., Larsen J., Skov R., Schonheyder H.C. Gentamicin-resistant Enterococcus faecalis sequence type 6 with reduced penicillin susceptibility: diagnostic and therapeutic implications. J. Clin. Microbiol. 2010, 48:3820-3821.
14. Hiraga N., Muratani T., Naito S., Matsumoto T. Genetic analysis of faropenem-resistant Enterococcus faecalis in urinary isolates. J. Antibiot. 2008, 61:213-221.
15. Hollenbeck B.L., Rice L.B. Intrinsic and acquired resistance mechanisms in Enterococcus. Virulence 2012, 3:421-433.
16. Hsieh S.E., Hsu L.L., Hsu W.H., Chen C.Y., Liao C.T. Importance of amino acid alterations and expression of penicillin-binding protein 5 to ampicillin resistance of Enterococcus faecium in Taiwan. Int. J. Antimicrob. Agents 2006, 28:514-519.
17. Kawalec M., Pietras Z., Danilowicz E., Jakubczak A., Gniadkowski M., Hryniewicz W., Willems R.J.L. Clonal structure of Enterococcus faecalis isolated from Polish hospitals: characterization of epidemic clones. J. Clin. Microbiol. 2007, 45(1):147-153.
18. Kuch A., Willems R.J., Werner G., Coque T.M., Hammerum A.M., Sundsfjord A., Klare I., Ruiz-Garbajosa P., Simonsen G.S., van Luit-Asbroek M., Hryniewicz W., Sadowy E. Insight into antimicrobial susceptibility and population structure of contemporary human Enterococcus faecalis isolates from Europe. J. Antimicrob. Chemother. 2012, 67:551-558.
19. Metzidie E., Manolis E.M., Pournaras S., Sofianou D., Tsakris A. Spread of an unusual penicillin and imipenem resistant but ampicillin-susceptible phenotype among Enterococcus faecalis clinical isolates. J. Antimicrob. Chemother. 2005, 57:158-160.
20. Murray B.E. The life and times of the Enterococcus. Clin. Microbiol. Rev. 1990, 3(1):45-65.
21. Ono S., Muratani T., Matsumoto T. Mechanisms of resistance to imipenem and ampicillin in Enterococcus faecalis. Antimicrob. Agents Chemother. 2005, 49:2954-2958.
22. Penas P.P., Mayer M.P.A., Gomes B.P.F.A., Endo M., Pignatari A.C., Bauab K.C., Pinheiro E.T. Analysis of genetic lineages and their correlation with virulence genes in Enterococcus faecalis clinical isolates from root canal and systemic infections. J. Endod. 2013, 39:858-864.
23. Pitondo-Silva A., Minarini L.A.R., Camargo I.L.B.C., Darini A.L. Clonal relationships determined by multilocus sequence typing among enteropathogenic Escherichia coli isolated in Brazil. Can. J. Microbiol. 2009, 55:672-679.
24. Ruiz-Garbajosa P., Bonten M.J.M., Robinson D.A., Top J., Nallapareddy S.R., Torres C., Coque T.M., Cantón R., Baquero F., Murray B.E., del Campo R., Willems R.J.L. Multilocus sequence typing scheme for Enterococcus faecalis reveals hospital-adapted genetic complexes in a background of high rates of recombination. J. Clin. Microbiol. 2006, 44(6):2220-2228.
25. Sader H.S., Jones R.N., Gales A.C., Silva J.B., Pignatari A.C. SENTRY Antimicrobial Surveillance Program Report: Latin American and Brazilian result for 1997 through 2001. Braz. J. Infect. Dis. 2004, 8(1):25-79.
26. Sauvage E., Kerff F., Terrak M., Ayala J.A., Charlier P. The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis. FEMS Microbiol. Rev. 2008, 32:234-258.
27. Signoretto C., Canepari P. Paradoxical effect of inserting, in Enterococcus faecalis penicillin-binding protein 5, an amino acid box responsible for low affinity for penicillin in Enterococcus faecium. Arch. Microbiol. 2000, 173:213-219.
28. Teixeira L.M., Carvalho M.G., Shewmaker P.L., Facklam R.R. Enterococcus. Manual of Clinical Microbiology 2011, 350-364. American Society for Microbiology Press, Washington, DC. 10th ed.
29. Weinstein M.P., Mirrett S., Kannangara S., Monahan J., Harrell L.J., Wilson A.C., Reller B. Multicenter evaluation of use of penicillin and ampicillin as surrogates for in vitro testing of susceptibility of enterococci to imipenem. J. Clin. Microbiol. 2004, 42:3747-3751.
30. Zapun A., Contreras-Martel C., Vernet T. Penicillin-binding proteins and β-lactam resistance. FEMS Microbiol. Rev. 2008, 32:361-385.