A rapid quantitative activity assay shows that the Vibrio cholerae colonization factor GbpA is an active lytic polysaccharide monooxygenase

FEBS Letters

Volumn 588, Issue 18, 2014, Pages 3435-3440

  Loose, Jennifer S M ^a   Forsberg, Zarah ^a   Fraaije, Marco W ^b   Eijsink, Vincent G H ^a   Vaaje Kolstad, Gustav ^a  

^a NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CHITIN; COLONIZATION FACTOR ANTIGENS; FIMBRIA PROTEIN; LYTIC POLYSACCHARIDE MONOOXYGENASE; MIXED FUNCTION OXIDASE;

AA10; AA11; AA9; ARTICLE; CHEMISTRY; COLONIZATION FACTOR; ENZYME ASSAY; ENZYMOLOGY; GBPA; OXIDATION REDUCTION REACTION; STANDARD; VIBRIO CHOLERAE;

AA10; AA11; AA9; COLONIZATION FACTOR; GBPA; LYTIC POLYSACCHARIDE MONOOXYGENASE; VIBRIO CHOLERAE;

BACTERIAL PROTEINS; CHITIN; ENZYME ASSAYS; FIMBRIAE PROTEINS; MIXED FUNCTION OXYGENASES; OXIDATION-REDUCTION; REFERENCE STANDARDS; VIBRIO CHOLERAE;

EID: 84908688781     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2014.07.036     Document Type: Article

References (27)

1. A. Levasseur, E. Drula, V. Lombard, P.M. Coutinho, and B. Henrissat Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes Biotechnol. Biofuels 6 2013 41
2. Z. Forsberg, A.K. Mackenzie, M. Sørlie, A.K. Røhr, R. Helland, A.S. Arvai, G. Vaaje-Kolstad, and V.G.H. Eijsink Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases Proc. Natl. Acad. Sci. USA 111 2014 8446 8451
3. G.R. Hemsworth, G.J. Davies, and P.H. Walton Recent insights into copper-containing lytic polysaccharide mono-oxygenases Curr. Opin. Struct. Biol. 23 2013 660 668
4. G.R. Hemsworth, E.J. Taylor, R.Q. Kim, R.C. Gregory, S.J. Lewis, J.P. Turkenburg, A. Parkin, G.J. Davies, and P.H. Walton The copper active site of CBM33 polysaccharide oxygenases J. Am. Chem. Soc. 135 2013 6069 6077
5. R.J. Quinlan, M.D. Sweeney, L. Lo Leggio, H. Otten, J.C. Poulsen, K.S. Johansen, K.B. Krogh, C.I. Jorgensen, M. Tovborg, A. Anthonsen, T. Tryfona, C.P. Walter, P. Dupree, F. Xu, G.J. Davies, and P.H. Walton Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components Proc. Natl. Acad. Sci. USA 108 2011 15079 15084
6. W.T. Beeson, C.M. Phillips, J.H. Cate, and M.A. Marletta Oxidative cleavage of cellulose by fungal copper-dependent polysaccharide monooxygenases J. Am. Chem. Soc. 134 2012 890 892
7. Z. Forsberg, G. Vaaje-Kolstad, B. Westereng, A.C. Bunæs, Y. Stenstrøm, A. Mackenzie, M. Sørlie, S.J. Horn, and V.G.H. Eijsink Cleavage of cellulose by a CBM33 protein Protein Sci. 20 2011 1479 1483
8. C.M. Phillips, W.T. Beeson, J.H. Cate, and M.A. Marletta Cellobiose dehydrogenase and a copper-dependent polysaccharide monooxygenase potentiate cellulose degradation by Neurospora crassa ACS Chem. Biol. 6 2011 1399 1406
9. G. Vaaje-Kolstad, B. Westereng, S.J. Horn, Z.L. Liu, H. Zhai, M. Sørlie, and V.G.H. Eijsink An oxidative enzyme boosting the enzymatic conversion of recalcitrant polysaccharides Science 330 2010 219 222
10. S. Kim, J. Ståhlberg, M. Sandgren, R.S. Paton, and G.T. Beckham Quantum mechanical calculations suggest that lytic polysaccharide monooxygenases use a copper-oxyl, oxygen-rebound mechanism Proc. Natl. Acad. Sci. USA 111 2014 149 154
11. 2 by the mononuclear Cu center in polysaccharide monooxygenases Proc. Natl. Acad. Sci. USA 111 2014 8797 8802
12. R.F. Frederiksen, D.K. Paspaliari, T. Larsen, B.G. Storgaard, M.H. Larsen, H. Ingmer, M.M. Palcic, and J.J. Leisner Bacterial chitinases and chitin-binding proteins as virulence factors Microbiology 159 2013 833 847
13. R. Bhowmick, A. Ghosal, B. Das, H. Koley, D.R. Saha, S. Ganguly, R.K. Nandy, R.K. Bhadra, and N.S. Chatterjee Intestinal adherence of Vibrio cholerae involves a coordinated interaction between colonization factor GbpA and mucin Infect. Immun. 76 2008 4968 4977
14. T.J. Kirn, B.A. Jude, and R.K. Taylor A colonization factor links Vibrio cholerae environmental survival and human infection Nature 438 2005 863 866
15. E. Wong, G. Vaaje-Kolstad, A. Ghosh, R. Hurtado-Guerrero, P.V. Konarev, A.F.M. Ibrahim, D.I. Svergun, V.G.H. Eijsink, N.S. Chatterjee, and D.M.F. van Aalten The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces PLoS Pathog. 8 2012 1
16. M. Zampini, C. Pruzzo, V.P. Bondre, R. Tarsi, M. Cosmo, A. Bacciaglia, A. Chhabra, R. Srivastava, and B.S. Srivastava Vibrio cholerae persistence in aquatic environments and colonization of intestinal cells: involvement of a common adhesion mechanism FEMS Microbiol. Lett. 244 2005 267 273
17. R. Kittl, D. Kracher, D. Burgstaller, D. Haltrich, and R. Ludwig Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay Biotechnol. Biofuels 5 2012 79
18. D.P. Heuts, R.T. Winter, G.E. Damsma, D.B. Janssen, and M.W. Fraaije The role of double covalent flavin binding in chito-oligosaccharide oxidase from Fusarium graminearum Biochem. J. 413 2008 175 183
19. A.R. Ferrari, Y. Gaber, and M.W. Fraaije A fast, sensitive and easy colorimetric assay for chitinase and cellulase activity detection Biotechnol. Biofuels 7 2014 37
20. Y. Fan, T. Saito, and A. Isogai Preparation of chitin nanofibers from squid pen beta-chitin by simple mechanical treatment under acid conditions Biomacromolecules 9 2008 1919 1923
21. J.W. Agger, T. Isaksen, A. Varnai, S. Vidal-Melgosa, W.G. Willats, R. Ludwig, S.J. Horn, V.G.H. Eijsink, and B. Westereng Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant cell wall degradation Proc. Natl. Acad. Sci. USA 111 2014 6287 6292
22. S. Drouillard, S. Armand, G.J. Davies, C.E. Vorgias, and B. Henrissat Serratia marcescens chitobiase is a retaining glycosidase utilizing substrate acetamido group participation Biochem. J. 328 1997 945 949
23. I. Tews, A. Perrakis, A. Oppenheim, Z. Dauter, K.S. Wilson, and C.E. Vorgias Bacterial chitobiase structure provides insight into catalytic mechanism and the basis of Tay-Sachs disease Nat. Struct. Biol. 3 1996 638 648
24. G. Vaaje-Kolstad, S.J. Horn, M. Sørlie, and V.G.H. Eijsink The chitinolytic machinery of Serratia marcescens - a model system for enzymatic degradation of recalcitrant polysaccharides FEBS J. 280 2013 3028 3049
25. F.L. Aachmann, M. Sørlie, G. Skjåk-Bræk, V.G.H. Eijsink, and G. Vaaje-Kolstad NMR structure of a lytic polysaccharide monooxygenase provides insight into copper binding, protein dynamics, and substrate interactions Proc. Natl. Acad. Sci. USA 109 2012 18779 18784
26. Z. Forsberg, A.K. Røhr, S. Mekasha, K.K. Andersson, V.G.H. Eijsink, G. Vaaje-Kolstad, and M. Sørlie Comparative study of two chitin-active and two cellulose-active AA10-type lytic polysaccharide monooxygenases Biochemistry 53 2014 1647 1656
27. G.R. Buettner Ascorbate autoxidation in the presence of iron and copper chelates Free Radic. Res. Commun. 1 1986 349 353