Label-free fluorometric detection of S1 nuclease activity by using polycytosine oligonucleotide-templated silver nanoclusters

Analytical Biochemistry

Volumn 468, Issue , 2015, Pages 34-38

  Wang, Lihui ^a   Ma, Keke ^a   Zhang, Yaodong ^a  

^a SHAANXI NORMAL UNIVERSITY   (China)

Author keywords

Enzyme activity; Enzyme inhibitor; Fluorometry; S1 nuclease; Silver nanoclusters

Indexed keywords

ENZYME ACTIVITY; FLUORESCENCE; NANOCLUSTERS; OLIGONUCLEOTIDES;

ENZYME INHIBITORS; FLUORESCENT METHODS; FLUOROMETRIC DETECTION; FLUOROMETRY; INHIBITOR SCREENINGS; POLYCYTOSINE OLIGONUCLEOTIDES; S1 NUCLEASE; SILVER NANOCLUSTERS;

SILVER;

CYTOSINE; NUCLEASE S1; OLIGONUCLEOTIDE; POLYCYTOSINE OLIGONUCLEOTIDE; PYROPHOSPHATE; SILVER NANOPARTICLE; UNCLASSIFIED DRUG; DIPHOSPHORIC ACID; ENDONUCLEASE S1, ASPERGILLUS; ENZYME INHIBITOR; FLUORESCENT DYE; FUNGAL PROTEIN; METAL NANOPARTICLE; POLYCYTIDYLIC ACID; PYROPHOSPHORIC ACID DERIVATIVE; SILVER; SINGLE STRANDED DNA;

ARTICLE; ENZYME ACTIVITY; FLUORESCENCE; FLUOROMETRY; PRIMED IN SITU LABELING; ANTAGONISTS AND INHIBITORS; CHEMISTRY; FEASIBILITY STUDY; METABOLISM; PROCEDURES; SPECTROFLUOROMETRY;

DIPHOSPHATES; DNA, SINGLE-STRANDED; ENZYME INHIBITORS; FEASIBILITY STUDIES; FLUORESCENT DYES; FUNGAL PROTEINS; METAL NANOPARTICLES; OLIGONUCLEOTIDES; POLY C; SILVER; SINGLE-STRAND SPECIFIC DNA AND RNA ENDONUCLEASES; SPECTROMETRY, FLUORESCENCE;

EID: 84908621713     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.09.011     Document Type: Article

References (33)

1. R.C. Wiegand, G.N. Godson, C.M. Radding, Specificity of the S1 nuclease from Aspergillus oryzae, J. Biol. Chem. 250 (1975) 8848-8855.
2. N.A. Desai, V. Shankar, Single-strand-specific nucleases, FEMS Microbiol. Rev. 26 (2003) 457-491.
3. X. Yang, F. Pu, J. Ren, X. Qu, DNA-templated ensemble for label-free and real-time fluorescence turn-on detection of enzymatic/oxidative cleavage of single-stranded DNA, Chem. Commun. 47 (2011) 8133-8135 (Camb.).
4. J.L. Butour, A.M. Mazard, C. Vieussens, N.P. Johnson, Kinetic studies of the hydrolysis of platinum-DNA complexes by nuclease S1, Chem. Biol. Interact. 73 (1990) 195-205.
5. B. Mouratou, S. Rouyre, S. Pauillac, J.L. Guesdon, Development of nonradioactive microtiter plate assays for nuclease activity, Anal. Biochem. 309 (2002) 40-47.
6. R. Cao, B. Li, Y. Zhang, Z. Zhang, Naked-eye sensitive detection of nuclease activity using positively-charged gold nanoparticles as colorimetric probes, Chem. Commun. 47 (2011) 12301-12303 (Camb.).
7. J. Ding, W. Qin, Potentiometric sensing of nuclease activities and oxidative damage of single-stranded DNA using a polycation-sensitive membrane electrode, Biosens. Bioelectron. 47 (2013) 559-565.
8. S.C. Hillier, C.G. Frost, A.T. Jenkins, H.T. Braven, R.W. Keay, S.E. Flower, J.M. Clarkson, An electrochemical study of enzymatic oligonucleotide digestion, Bioelectrochem. Bioenerg. 63 (2004) 307-310.
9. Z. Zhou, J. Zhu, L. Zhang, Y. Du, S. Dong, E. Wang, G-quadruplex-based fluorescent assay of S1 nuclease activity and K+, Anal. Chem. 85 (2013) 2431-2435.
10. Y. He, B. Jiao, H. Tang, Interaction of single-stranded DNA with graphene oxide: fluorescence study and its application for S1 nuclease detection, RSC Adv. 4 (2014) 18294-18300.
11. Z.D. Liu, P.P. Hu, H.X. Zhao, Y.F. Li, C.Z. Huang, Carbon nanotube-DNA hybrid used for activity monitoring and inhibitor screening of nuclease, Anal. Chim. Acta 706 (2011) 171-175.
12. Y. Tang, F. Feng, F. He, S. Wang, Y. Li, D. Zhu, Direct visualization of enzymatic cleavage and oxidative damage by hydroxyl radicals of single-stranded DNA with a cationic polythiophene derivative, J. Am. Chem. Soc. 128 (2006) 14972-14976.
13. R. Jin, Quantum sized, thiolate-protected gold nanoclusters, Nanoscale 2 (2010) 343-362.
14. J. Lee, J. Park, H.H. Lee, H.I. Kim, W.J. Kim, DNA-templated silver nanoclusters as label-free, sensitive detection probes for potassium ion and nitric oxide, J. Mater. Chem. B 2 (2014) 2616-2621.
15. M. Zhang, S.M. Guo, Y.R. Li, P. Zuo, B.C. Ye, A label-free fluorescent molecular beacon based on DNA-templated silver nanoclusters for detection of adenosine and adenosine deaminase, Chem. Commun. 48 (2012) 5488-5490 (Camb.).
16. M. Zhang, Y.Q. Liu, C.Y. Yu, B.C. Yin, B.C. Ye, Multiplexed detection of microRNAs by tuning DNA-scaffolded silver nanoclusters, Analyst 138 (2013) 4812-4817.
17. X. Xia, Y. Long, J. Wang, Glucose oxidase-functionalized fluorescent gold nanoclusters as probes for glucose, Anal. Chim. Acta 772 (2013) 81-86.
18. Y. Qian, Y. Zhang, L. Lu, Y. Cai, A label-free DNA-templated silver nanocluster probe for fluorescence on-off detection of endonuclease activity and inhibition, Biosens. Bioelectron. 51 (2014) 408-412.
19. K. Zhang, K. Wang, M. Xie, X. Zhu, L. Xu, R. Yang, B. Huang, X. Zhu, DNAtemplated silver nanoclusters based label-free fluorescent molecular beacon for the detection of adenosine deaminase, Biosens. Bioelectron. 52 (2014) 124-128.
20. D. Schultz, S.M. Copp, N. Markesevic, K. Gardner, S.S. Oemrawsingh, D. Bouwmeester, E. Gwinn, Dual-color nanoscale assemblies of structurally stable, few-atom silver clusters, as reported by fluorescence resonance energy transfer, ACS Nano 7 (2013) 9798-9807.
21. L. Zhang, J. Zhu, Z. Zhou, S. Guo, J. Li, S. Dong, E. Wang, A new approach to light up DNA/Ag nanocluster-based beacons for bioanalysis, Chem. Sci. 4 (2013) 4004-4010.
22. A. Desireddy, B.E. Conn, J. Guo, B. Yoon, R.N. Barnett, B.M. Monahan, K. Kirschbaum, W.P. Griffith, R.L. Whetten, U. Landman, Ultrastable silver nanoparticles, Nature 501 (2013) 399-402.
23. J.T. Petty, J. Zheng, N.V. Hud, R.M. Dickson, DNA-templated Ag nanocluster formation, J. Am. Chem. Soc. 126 (2004) 5207-5212.
24. D. Schultz, K. Gardner, S.S. Oemrawsingh, N. Markesevic, K. Olsson, M. Debord, D. Bouwmeester, E. Gwinn, Evidence for rod-shaped DNA-stabilized silver nanocluster emitters, Adv. Mater. 25 (2013) 2797-2803.
25. J. Xie, Y. Zheng, J.Y. Ying, Protein-directed synthesis of highly fluorescent gold nanoclusters, J. Am. Chem. Soc. 131 (2009) 888-889.
26. J. Zheng, R.M. Dickson, Individual water-soluble dendrimer-encapsulated silver nanodot fluorescence, J. Am. Chem. Soc. 124 (2002) 13982-13983.
27. J. Zheng, C. Zhang, R.M. Dickson, Highly fluorescent, water-soluble, size-tunable gold quantum dots, Phys. Rev. Lett. 93 (2004) 077402.
28. J. Liu, DNA-stabilized, fluorescent, metal nanoclusters for biosensor development, Trends Anal. Chem. 58 (2014) 99-111.
29. R. Hu, Y.R. Liu, R.M. Kong, M.J. Donovan, X.B. Zhang, W. Tan, G.L. Shen, R.Q. Yu, Double-strand DNA-templated formation of copper nanoparticles as fluorescent probe for label free nuclease enzyme detection, Biosens. Bioelectron. 42 (2013) 31-35.
30. Z. Qing, X. He, T. Qing, K. Wang, H. Shi, D. He, Z. Zou, L. Yan, F. Xu, X. Ye, Z. Mao, Poly(thymine)-templated fluorescent copper nanoparticles for ultrasensitive label-free nuclease assay and its inhibitors screening, Anal. Chem. 85 (2013) 12138-12143.
31. C.M. Ritchie, K.R. Johnsen, J.R. Kiser, Y. Antoku, R.M. Dickson, J.T. Petty, Ag nanocluster formation using a cytosine oligonucleotide template, J. Phys. Chem. C 111 (2007) 175-181.
32. Y. Zhang, Y. Cai, Z. Qi, L. Lu, Y. Qian, DNA-templated silver nanoclusters for fluorescence turn-on assay of acetylcholinesterase activity, Anal. Chem. 85 (2013) 8455-8461.
33. M. Wang, X. Gu, G. Zhang, D. Zhang, D. Zhu, Continuous colorimetric assay for acetylcholinesterase and inhibitor screening with gold nanoparticles, Langmuir 25 (2009) 2504-2507.