Pigments, elemental composition (C, N, P, and Si), and stoichiometry of particulate matter in the naturally iron fertilized region of Kerguelen in the Southern Ocean

Biogeosciences

Volumn 11, Issue 20, 2014, Pages 5931-5955

  Lasbleiz, M ^a   Leblanc, K ^a   Blain, S ^b,c   Ras, J ^b,d   Cornet Barthaux, V ^a   Helias Nunige S ^a   Queguiner B ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

^b SORBONNE UNIVERSITÉ   (France)

^c LABORATOIRE ARAGO   (France)

^d CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALGAL BLOOM; BIOMASS; CARBON; COMMUNITY STRUCTURE; IRON; NITROGEN; PHOSPHORUS; PHYTOPLANKTON; PIGMENT; SILICON; SPATIAL DISTRIBUTION; STOICHIOMETRY;

FRENCH SOUTHERN TERRITORIES; KERGUELEN; KERGUELEN ISLANDS; SOUTHERN OCEAN;

EID: 84908612202     PISSN: 17264170     EISSN: 17264189     Source Type: Journal    
DOI: 10.5194/bg-11-5931-2014     Document Type: Article

References (117)

1. Armand, L. K., Cornet-Barthaux, V., Mosseri, J., and Quéguiner, B.: Late summer diatom biomass and community structure on and around the naturally iron-fertilised Kerguelen Plateau in the Southern Ocean, Deep-Sea Res. Pt. II, 55, 653-676, doi:10.1016/j.dsr2.2007.12.031, 2008.
2. Armstrong, R. A., Peterson, M. L., Lee, C., and Wakeham, S. G.: Settling velocity spectra and the ballast ratio hypothesis, Deep-Sea Res. Pt. II, 56, 1470-1478, doi:10.1016/j.dsr2.2008.11.032, 2009.
3. Arrigo, K. R. and Alderkamp, A.-C.: Shedding dynamic light on Fe limitation (DynaLiFe), Deep-Sea Res. Pt. II, 71-76, 1-4, doi:10.1016/j.dsr2.2012.03.004, 2012.
4. Arrigo, K. R., Dunbar, R. B., Lizotte, M. P., and Robinson, D. H.: Taxon-specific differences in C/P and N/P drawdown for phyto-plankton in the Ross Sea, Antarctica, Geophys. Res. Lett., 29, 1938, doi:10.1029/2002gl015277, 2002.
5. Assmy, P., Smetacek, V., Montresor, M., Klaas, C., Henjes, J., Strass, V. H., Arrieta, J. M., Bathmann, U., Berg, G. M., Breitbarth, E., Cisewski, B., Friedrichs, L., Fuchs, N., Herndl, G. J., Jansen, S., Krägefsky, S., Latasa, M., Peeken, I., Rüttgers, R., Scharek, R., Schüller, S. E., Steigenberger, S., Webb, A., and Wolf-Gladrow, D.: Thick-shelled, grazer-protected diatoms decouple ocean carbon and silicon cycles in the iron-limited Antarctic Circumpolar Current, P. Natl. Acad Sci. USA, 110, 20633-20638, 2013.
6. Banse, K.: Should we continue to use the 1% light depth convention for estimating the compensation depth of phytoplankton for another 70 years?, Limnol. Oceanog. Bullet., 13, 49-51, 2004.
7. Bathmann, U. V., Scharek, R., Klaas, C., Dubischar, C. D., and Smetacek, V.: Spring development of phytoplankton biomass and composition in major water masses of the Atlantic sector of the Southern Ocean, Deep-Sea Res. Pt. II, 44, 51-67, doi:10.1016/S0967-0645(96)00063-X, 1997.
8. Blain, S., Tréguer, P., Belviso, S., Bucciarelli, E., Denis, M., Desabre, S., Fiala, M., Martin Jézéquel, V., Le Fèvre, J., Mayzaud, P., Marty, J.-C., and Razouls, S.: A biogeochemical study of the island mass effect in the context of the iron hypothesis: Kerguelen Islands, Southern Ocean, Deep-Sea Res. Pt. I, 48, 163-187, doi:10.1016/S0967-0637(00)00047-9, 2001.
9. Blain, S., Queguiner, B., Armand, L., Belviso, S., Bombled, B., Bopp, L., Bowie, A., Brunet, C., Brussaard, C., Carlotti, F., Christaki, U., Corbiere, A., Durand, I., Ebersbach, F., Fuda, J.-L., Garcia, N., Gerringa, L., Griffiths, B., Guigue, C., Guillerm, C., Jacquet, S., Jeandel, C., Laan, P., Lefevre, D., Lo Monaco, C., Malits, A., Mosseri, J., Obernosterer, I., Park, Y.-H., Picheral, M., Pondaven, P., Remenyi, T., Sandroni, V., Sarthou, G., Savoye, N., Scouarnec, L., Souhaut, M., Thuiller, D., Timmermans, K., Trull, T., Uitz, J., van Beek, P., Veldhuis, M., Vincent, D., Viollier, E., Vong, L., and Wagener, T.: Effect of natural iron fertilization on carbon sequestration in the Southern Ocean, Nature, 446, 1070-1074, doi:10.1038/nature05700, 2007.
10. Blain, S., Capparos, J., Guéneuguès, A., Obernosterer, I., and Oriol, L.: Distributions and stoichiometry of dissolved nitrogen and phosphorus in the iron fertilized region near Kergue-len (Southern Ocean), Biogeosciences Discuss., 11, 9949-9977, doi:10.5194/bgd-11-9949-2014, 2014.
11. Bowie, A. W., van der Merwe, P., Trull, T., Quéroué, F., Fourquez, M., Planchon, F., Sarthou, G., and Blain, S.: Iron budgets for three distinct biogeochemical sites around the Kerguelen plateau (Southern Ocean) during the natural fertilization experiment KEOPS-2, Biogeosciences, in preparation, 2014.
12. Boyd, P. W.: Environmental factors controlling phytoplankton processes in the Southern Ocean, J. Phycol., 38, 844-861, doi:10.1046/j.1529-8817.2002.t01-1-01203.x, 2002.
13. Boyd, P. W.: Biogeochemistry: Iron findings, Nature, 446, 989-991, doi:10.1038/446989a, 2007.
14. Boyd, P. W.: Diatom traits regulate Southern Ocean silica leakage, P. Natl. Acad. Sci. USA, 110, 20358-20359, doi:10.1073/pnas.1320327110, 2013.
15. Boyd, P. W., LaRoche, J., Gall, M., Frew, R., and McKay, R. M. L.: Role of iron, light, and silicate in controlling algal biomass in subantarctic waters SE of New Zealand, J. Geophys. Res.-Oceans, 104, 13395-13408, doi:10.1029/1999jc900009, 1999.
16. Brzezinski, M. A.: The Si:C:N ratio of marine diatoms: interspecific variability and the effect of some environmental variables, J. Phycol., 21, 347-357, 1985.
17. Brzezinski, M. A., Nelson, D. M., Franck, V. M., and Sigmon, D. E.: Silicon dynamics within an intense open-ocean diatom bloom in the Pacific sector of the Southern Ocean, Deep-Sea Res. Pt. II, 48, 3997-4018, doi:10.1016/S0967-0645(01)00078-9, 2001.
18. Bucciarelli, E., Blain, S., and Tréguer, P.: Iron and manganese in the wake of the Kerguelen Islands (Southern Ocean), Mar. Chem., 73, 21-36, doi:10.1016/S0304-4203(00)00070-0, 2001.
19. Buesseler, K. O.: The decoupling of production and particulate export in the surface ocean, Global Biogeochem. Cy., 12, 297-310, doi:10.1029/97gb03366, 1998.
20. Cailliau, C., Claustre, H., and Giannino, S.: Chemotaxonomic analysis of phytoplankton distribution in the Indian sector of the Southern Ocean during late austral summer, Oceanolica Acta, 20, 721-732, 1997.
21. Carlotti, F., Nowaczyk, A., Jouandet, M.-P., Lefèvre, D., and Harmelin, M.: Mesozooplankton structure and functioning during the onset of the Kerguelen bloom during KEOP2 survey, Bio-geosciences, in preparation, 2014.
22. Cassar, N., Bender, M. L., Barnett, B. A., Fan, S., Moxim, W. J., Levy, H., and Tilbrook, B.: The southern ocean biological response to aeolian iron deposition, Science, 317, 1067-1070, doi:10.1126/science.1144602, 2007.
23. Cavagna, A. J., Lefèvre, D., Dehairs, F., Elskens, M., Fripiat, F., Closset, I., Lasbleiz, M., Flores-Leive, L., Cardinal, D., Leblanc, K., Fernandez, C., Oriol, L., Blain, S., and Quéguiner, B.: Biological productivity regime in the surface waters around the Ker-guelen Island in the Southern Ocean - from the use of an inte-grative approach, Biogeosciences, in preparation, 2014.
24. Christaki, U., Lefèvre, D., Georges, C., Colombet, J., Catala, P., Courties, C., Sime-Ngando, T., Blain, S., and Obernos-terer, I.: Microbial food web dynamics during spring phyto-plankton blooms in the naturally iron-fertilized Kerguelen area (Southern Ocean), Biogeosciences Discuss., 11, 6985-7028, doi:10.5194/bgd-11-6985-2014, 2014.
25. Claustre, H.: The trophic status of various oceanic provinces as revealed by phytoplankton pigment signatures, Limnol. Oceanogr., 39, 1206-1210, doi:10.4319/lo.1994.39.5.1206, 1994.
26. Closset, I., Lasbleiz, M., Leblanc, K., Quéguiner, B., Cavagna, A.-J., Elskens, M., Navez, J., and Cardinal, D.: Seasonal evolution of net and regenerated silica production around a natural Fe-fertilized area in the Southern Ocean estimated from Si isotopic approaches, Biogeosciences Discuss., 11, 6329-6381, doi:10.5194/bgd-11-6329-2014, 2014.
27. Copin-Montegut, C. and Copin-Montegut, G.: The chemistry of particulate matter from the south Indian and Antarctic oceans, Deep-Sea Res., 25, 911-931, doi:10.1016/0146-6291(78)90633-1, 1978.
28. Cullen, J. J.: The Deep Chlorophyll Maximum: Comparing Vertical Profiles of Chlorophyll a, Can. J. Fish. Aquat. Sci., 39, 791-803, doi:10.1139/f82-108, 1982.
29. d'Ovidio, F., Zhou, M., Park, Y. H., Nencioli, F., Resplandy, L., Doglioli, A., Petrenko, A., Blain, S., and Queguiner, B.: Guiding biogeochemical campaigns with high resolution altimetry: waiting for the SWOT mission, Proceedings of 20 Years of Progress in Radar Altimetry Symposium, Venice, Italy, 2012.
30. de Baar, H. J. W., Van Leeuwe, M. A., Scharek, R., Goeyens, L., Bakker, K. M. J., and Fritsche, P.: Nutrient anomalies in Fragilariopsis kerguelensis blooms, iron deficiency and the nitrate/phosphate ratio of the Antarctic Ocean, edited by: Redfield, A. C., Deep-Sea Res. Pt. II, 44, 229-260, doi:10.1016/S0967-0645(96)00102-6, 1997.
31. de Baar, H. J. W., Boyd, P., Coale, K., Landry, M., Tsuda, A., Assmy, P., Bakker, D. C., Bozec, Y., Barber, R. T., Brzezinski, M., Buesseler, K., Boyé, M., Croot, P., Gervais, F., Gorbunov, M., Harrison, P., Hiscock, W., Laan, P., Lancelot, C., Law, C., Levasseur, M., Marchetti, A., Millero, F., Nishioka, J., Nojiri, Y., van Oijen, T., Riebesell, U., Rijkenberg, M., Saito, H., Takeda, S., Timmermans, K., Veldhuis, M., Waite, A., and Wong, C.-S.: Synthesis of iron fertilization experiments: From the Iron Age in the Age of Enlightenment, J. Geophys. Res., 110, C09S16, doi:10.1029/2004JC002601, 2005.
32. Dugdale, R. C., Wilkerson, F. P., and Minas, H. J.: The role of a silicate pump in driving new production, Deep-Sea Res. Pt. I, 42, 697-719, doi:10.1016/0967-0637(95)00015-X, 1995.
33. Ediger, D., Raine, R., Weeks, A. R., Robinson, I. S., and Sagan, S.: Pigment signatures reveal temporal and regional differences in taxonomic phytoplankton composition off the west coast of Ireland, J. Plankton Rese., 23, 893-902, doi:10.1093/plankt/23.8.893, 2001.
34. Falkowski, P. G., Laws, E. A., Barber, R. T., and Murray, J. W.: Phytoplankton and Their Role in Primary, New, and Export Production, in: Ocean Biogeochemistry, edited by: Fasham, M. R., Global Change - The IGBP Series, Springer Berlin Heidelberg, 99-121, 2003.
35. Firme, G. F., Rue, E. L., Weeks, D. A., Bruland, K. W., and Hutchins, D. A.: Spatial and temporal variability in phytoplank-ton iron limitation along the California coast and consequences for Si, N, and C biogeochemistry, Global Biogeochem. Cy., 17, 1016, doi:10.1029/2001gb001824, 2003.
36. Franck, V. M., Brzezinski, M. A., Coale, K. H., and Nelson, D. M.: Iron and silicic acid concentrations regulate Si uptake north and south of the Polar Frontal Zone in the Pacific Sector of the Southern Ocean, Deep-Sea Res. Pt. II, 47, 3315-3338, doi:10.1016/S0967-0645(00)00070-9, 2000.
37. Froneman, P. W., Pakhomov, E. A., and Balarin, M. G.: Size-fractionated phytoplankton biomass, production and biogenic carbon flux in the eastern Atlantic sector of the Southern Ocean in late austral summer 1997-1998, Deep-Sea Res. Pt. II, 51, 2715-2729, doi:10.1016/j.dsr2.2002.09.001, 2004.
38. Gall, M. P., Boyd, P. W., Hall, J., Safi, K. A., and Chang, H.: Phy-toplankton processes, Part 1: Community structure during the Southern Ocean Iron RElease Experiment (SOIREE), Deep-Sea Res. Pt. II, 48, 2551-2570, doi:10.1016/S0967-0645(01)00008-X, 2001.
39. Gautier, I.: Les basaltes des îles Kerguelen (Terres Australes et Antarctiques Françaises), Thèse de Doctorat, Université Paris VI, France, 383 pp., 1987.
40. Geider, R. and La Roche, J.: Redfield revisited: variability of C:N:P in marine microalgae and its biochemical basis, Europ. J. Phy-col., 37, 1-17, doi:10.1017/s0967026201003456, 2002.
41. Goffart, A., Catalano, G., and Hecq, J. H.: Factors controlling the distribution of diatoms and Phaeocystis in the Ross Sea, J. Marine Syst., 27, 161-175, doi:10.1016/S0924-7963(00)00065-8, 2000.
42. Hare, C. E., DiTullio, G. R., Trick, C. G., Bruland, S. W. W. W., Rue, E. L., and Hutchins, D. A.: Phytoplankton community structure changes following simulated upwelled iron inputs in the Peru upwelling region, Aquat. Microb. Ecol., 38, 269-282, doi:10.3354/ame038269, 2005.
43. Hare, C. E., DiTullio, G. R., Riseman, S. F., Crossley, A. C., Popels, L. C., Sedwick, P. N., and Hutchins, D. A.: Effects of changing continuous iron input rates on a Southern Ocean algal assemblage, Deep-Sea Res. Pt. I, 54, 732-746, doi:10.1016/j.dsr.2007.02.001, 2007.
44. Heimburger, A., Losno, R., Triquet, S., Dulac, F., and Mahowald, N.: Direct measurements of atmospheric iron, cobalt, and aluminum-derived dust deposition at Kerguelen Islands, Global Biogeochem. Cy., 26, GB4016, doi:10.1029/2012gb004301, 2012.
45. Henjes, J., Assmy, P., Klaas, C., Verity, P., and Smetacek, V.: Response of microzooplankton (protists and small cope-pods) to an iron-induced phytoplankton bloom in the Southern Ocean (EisenEx), Deep-Sea Res. Pt. I, 54, 363-384, doi:10.1016/j.dsr.2006.12.004, 2007.
46. Hoffmann, L. J., Peeken, I., Lochte, K., Assmy, P., and Veldhuis, M.: Different reactions of Southern Ocean phytoplankton size classes to iron fertilization., Limnol. Oceanogr., 51, 1217-1229, doi:10.4319/lo.2006.51.3.1217, 2006.
47. Hoffmann, L. J., Peeken, I., and Lochte, K.: Effects of iron on the elemental stoichiometry during EIFEX and in the diatoms Fragi-lariopsis kerguelensis and Chaetoceros dichaeta, Biogeosciences, 4, 569-579, doi:10.5194/bg-4-569-2007, 2007.
48. Howard, A. G., Coxhead, A. J., Potter, I. A., and Watt, A. P.: Determination of dissolved aluminium by the micelle-enhanced fluorescence of its lumogallion complex, Analyst, 111, 1379-1382, doi:10.1039/an9861101379, 1986.
49. Hutchins, D. A. and Bruland, K. W.: Iron-limited diatom growth and Si:N uptake ratios in a coastal upwelling regime, Nature, 393, 561-564, doi:10.1038/31203, 1998.
50. Hydes, D. J. and Liss, P. S.: Fluorimetric method for the determination of low concentrations of dissolved aluminium in natural waters, Analyst, 101, 922-931, doi:10.1039/an9760100922, 1976.
51. Jacquet, S. H. M., Dehairs, F., Cavagna, A. J., Planchon, F., Monin, L., André, L., Closset, I., and Cardinal, D.: Early season mesopelagic carbon remineralization and transfer efficiency in the naturally iron-fertilized Kerguelen area, Biogeosciences Discuss., 11, 9035-9069, doi:10.5194/bgd-11-9035-2014, 2014.
52. Johnsen, G. and Sakshaug, E.: Bio-optical characteristics and pho-toadaptive responses in the toxic and bloom-forming dinoflag-ellates Gyrodinium aureolum, Gymnodinium galatheanum, and two strains of Prorocentrum minimum, J. Phycol., 29, 627-642, doi:10.1111/j.0022-3646.1993.00627.x, 1993.
53. Jouandet, M. P., Blain, S., Metzl, N., Brunet, C., Trull, T. W., and Obernosterer, I.: A seasonal carbon budget for a naturally iron-fertilized bloom over the Kerguelen Plateau in the Southern Ocean, Deep-Sea Res. Pt. II, 55, 856-867, doi:10.1016/j.dsr2.2007.12.037, 2008.
54. Klausmeier, C. A., Litchman, E., and Levin, S. A.: Phyto-plankton growth and stoichiometry under multiple nutrient limitation, Limnol. Oceanogr., 49, 1463-1470, doi:10.4319/lo.2004.49.4-part-2.1463, 2004.
55. Kopczyńska, E. E., Fiala, M., and Jeandel, C.: Annual and inter-annual variability in phytoplankton at a permanent station off Kerguelen Islands, Southern Ocean, Polar Biol., 20, 342-351, doi:10.1007/s003000050312, 1998.
56. Lance, V. P., Hiscock, M. R., Hilting, A. K., Stuebe, D. A., Bidigare, R. R., Smith Jr, W. O., and Barber, R. T.: Primary productivity, differential size fraction and pigment composition responses in two Southern Ocean in situ iron enrichments, Deep-Sea Res. Pt. I, 54, 747-773, doi:10.1016/j.dsr.2007.02.008, 2007.
57. Lancelot, C., Hannon, E., Becquevort, S., Veth, C., and de Baar, H. J. W.: Modeling phytoplankton blooms and carbon export production in the Southern Ocean: dominant controls by light and iron in the Atlantic sector in Austral spring 1992, Deep-Sea Res. Pt. I, 47, 1621-1662, doi:10.1016/S0967-0637(00)00005-4, 2000.
58. Lasbleiz, M.: Biogeochemical cycles (Si, C, N, P) in relation to nutritional dynamics of phytoplankton in the iron-fertilized region of Kerguelen, Unpublished thesis, Aix-Marseille Université, France, 336 pp., 2014.
59. Laurenceau, E. C., Trull, T. W., Davies, D. M., Bray, S. G., Do-ran, J., Planchon, F., Carlotti, F., Jouandet, M.-P., Cavagna, A.-J., Waite, A. M., and Blain, S.: The relative importance of phytoplankton aggregates and zooplankton fecal pellets to carbon export: insights from free-drifting sediment trap deployments in naturally iron-fertilised waters near the Ker-guelen plateau, Biogeosciences Discuss., 11, 13623-13673, doi:10.5194/bgd-11-13623-2014, 2014.
60. Leblanc, K., Quéguiner, B., Fiala, M., Blain, S., Morvan, J., and Corvaisier, R.: Particulate biogenic silica and carbon production rates and particulate matter distribution in the Indian sector of the Subantarctic Ocean, Deep-Sea Res. Pt. II, 49, 3189-3206, doi:10.1016/S0967-0645(02)00078-4, 2002.
61. Legendre, L. and Le Fèvre, J.: Hydrodynamical singularities as controls of recycled versus export production in oceans in: Geological Journal, edited by: Berger, W. H., Smetacek, V. S., and Wefer, G., Productivity of the oceans: Present and past, Wiley, 49-63, 1989.
62. Lévy, M., Klein, P., and Treguier, A.-M.: Impact of sub-mesoscale physics on production and subduction of phytoplankton in an oligotrophic regime, J. Mar. Res., 59, 535-565, 2001.
63. Margalef, R.: Ecological Correlations and the Relationship Between Primary Productivity and Community Structure, in: Memorie dell'Istituto Italiano di Idrobiologia, 355-364, 1965.
64. Marra, J. F., Lance, V. P., Vaillancourt, R. D., and Hargreaves, B. R.: Resolving the ocean's euphotic zone, Deep-Sea Res. Pt. I, 83, 45-50, doi:10.1016/j.dsr.2013.09.005, 2014.
65. 2 change: The Iron Hypothesis, Paleoceanography, 5, 1-13, doi:10.1029/PA005i001p00001, 1990.
66. Martin, J. H., Gordon, R. M., and Fitzwater, S. E.: The case for iron, Journal Name: Limnol. Oceanogr., 36, 1793-1802, 1991.
67. Martiny, A. C., Pham, C. T. A., Primeau, F. W., Vrugt, J. A., Moore, J. K., Levin, S. A., and Lomas, M. W.: Strong latitudinal patterns in the elemental ratios of marine plankton and organic matter, Nat. Geosci., 6, 279-283, doi:10.1038/ngeo1757, 2013.
68. 2, Global Biogeochem. Cy., 16, 5-1- 5-23, doi:10.1029/2001gb001442, 2002.
69. Moore, C. M., Hickman, A. E., Poulton, A. J., Seeyave, S., and Lucas, M. I.: Iron-light interactions during the CROZet natural iron bloom and EXport experiment (CROZEX): Taxonomic responses and elemental stoichiometry, Deep-Sea Res. Pt. II, 54, 2066-2084, doi:10.1016/j.dsr2.2007.06.015, 2007.
70. Morris, P. J. and Charette, M. A.: A synthesis of upper ocean carbon and dissolved iron budgets for Southern Ocean natural iron fertilisation studies, Deep-Sea Res. Pt. II, 90, 147-157, doi:10.1016/j.dsr2.2013.02.001, 2013.
71. Mosseri, J., Quéguiner, B., Armand, L., and Cornet-Barthaux, V.: Impact of iron on silicon utilization by diatoms in the Southern Ocean: A case study of Si/N cycle decoupling in a naturally iron-enriched area, Deep-Sea Res. Pt. II, 55, 801-819, doi:10.1016/j.dsr2.2007.12.003, 2008.
72. Nelson, D. M., Tréguer, P., Brzezinski, M. A., Leynaert, A., and Quéguiner, B.: Production and dissolution of biogenic silica in the ocean: Revised global estimates, comparison with regional data and relationship to biogenic sedimentation, Global Bio-geochem. Cy., 9, 359-372, doi:10.1029/95gb01070, 1995.
73. Park, Y.-H. and Gamberoni, L.: Cross-frontal exchange of Antarctic Intermediate Water and Antarctic Bottom Water in the Crozet Basin, Deep-Sea Res. Pt. II, 44, 963-986, doi:10.1016/S0967-0645(97)00004-0, 1997.
74. Park, Y.-H., Fuda, J.-L., Durand, I., and Naveira Gara-bato, A. C.: Internal tides and vertical mixing over the Kerguelen Plateau, Deep-Sea Res. Pt. II, 55, 582-593, doi:10.1016/j.dsr2.2007.12.027, 2008a.
75. Park, Y.-H., Roquet, F., Durand, I., and Fuda, J.-L.: Large-scale circulation over and around the Northern Kerguelen Plateau, Deep-Sea Res. Pt. II, 55, 566-581, doi:10.1016/j.dsr2.2007.12.030, 2008b.
76. Park, Y.-H., Durand, I., Kestenare, E., Rougier, G., Zhou, M., d'Ovidio, F., and Lee, J.-H.: Polar Front around Kerguelen: An up-to-date determination and associated circulation of subsurface waters, Biogeosciences, in preparation, 2014.
77. Park, Y.-H., Durand, I., Kestenare, E., Rougier, G., Zhou, M., d'Ovidio, F., Cotté, C., and Lee, J.-H.: Polar Front around the Kerguelen Islands: An up-to-date determination and associated circulation of surface/subsurface waters, J. Geophys. Res. Oceans, 119, doi:10.1002/2014jc010061, 2014.
78. Peeken, I.: Photosynthetic pigment fingerprints as indicators of phy-toplankton biomass and development in different water masses of the Southern Ocean during austral spring, Deep-Sea Res. Pt. II, 44, 261-282, doi:10.1016/S0967-0645(96)00077-X, 1997.
79. 234Th based approach, Biogeo-sciences, in preparation, 2014.
80. Pollard, R. T., Salter, I., Sanders, R. J., Lucas, M. I., Moore, C. M., Mills, R. A., Statham, P. J., Allen, J. T., Baker, A. R., Bakker, D. C. E., Charette, M. A., Fielding, S., Fones, G. R., French, M., Hickman, A. E., Holland, R. J., Hughes, J. A., Jickells, T. D., Lampitt, R. S., Morris, P. J., Nedelec, F. H., Nielsdottir, M., Planquette, H., Popova, E. E., Poulton, A. J., Read, J. F., Seeyave, S., Smith, T., Stinchcombe, M., Taylor, S., Thomalla, S., Ven-ables, H. J., Williamson, R., and Zubkov, M. V.: Southern Ocean deep-water carbon export enhanced by natural iron fertilization, Nature, 457, 577-580, doi:10.1038/nature07716, 2009.
81. Price, N. M.: The elemental stoichiometry and composition of an iron-limited diatom, Limnol. Oceanogr., 50, 1159-1171, doi:10.4319/lo.2005.50.4.1159, 2005.
82. Pujo-Pay, M. and Raimbault, P.: Improvement of the wet-oxidation procedure for simultaneous determination of particulate organic nitrogen and phosphorus collected on filters, Mar. Ecol.-Prog. Ser., 105, 203-207, 1994.
83. Quéguiner, B.: Biogenic silica production in the Australian sector of the Subantarctic Zone of the Southern Ocean in late summer 1998, J. Geophys. Res.-Oceans, 106, 31627-31636, doi:10.1029/2000jc000249, 2001.
84. Quéguiner, B.: Iron fertilization and the structure of planktonic communities in high nutrient regions of the Southern Ocean, Deep-Sea Res. Pt. II, 90, 43-54, doi:10.1016/j.dsr2.2012.07.024, 2013.
85. Quéguiner, B. and Brzezinski, M. A.: Biogenic silica production rates and particulate organic matter distribution in the Atlantic sector of the Southern Ocean during austral spring 1992, Deep-Sea Res. Pt. II, 49, 1765-1786, doi:10.1016/S0967-0645(02)00011-5, 2002.
86. Quéguiner, B., Tréguer, P., Peeken, I., and Scharek, R.: Biogeo-chemical dynamics and the silicon cycle in the Atlantic sector of the Southern Ocean during austral spring 1992, Deep-Sea Res. Pt. II, 44, 69-89, doi:10.1016/S0967-0645(96)00066-5, 1997.
87. Quéroué, F., Sarthou, G., Chever, F., Van der Merwe, P., Lannuzel, D., Townsend, A., Bucciarelli, E., Planquette, H., Cheize, M., Blain, S., d'Ovidio, F., and Bowie, A.: A new study of natural Fe fertilization processes in the vicinity of the Kerguelen Islands (KEOPS2 experiment), Biogeosciences, in preparation, 2014.
88. Ragueneau, O., Savoye, N., Del Amo, Y., Cotten, J., Tardiveau, B., and Leynaert, A.: A new method for the measurement of biogenic silica in suspended matter of coastal waters: using Si:Al ratios to correct for the mineral interference, Cont. Shelf Res., 25, 697-710, doi:10.1016/j.csr.2004.09.017, 2005.
89. Ras, J., Claustre, H., and Uitz, J.: Spatial variability of phytoplank-ton pigment distributions in the Subtropical South Pacific Ocean: comparison between in situ and predicted data, Biogeosciences, 5, 353-369, doi:10.5194/bg-5-353-2008, 2008.
90. Read, J. F., Pollard, R. T., and Allen, J. T.: Sub-mesoscale structure and the development of an eddy in the Subantarctic Front north of the Crozet Islands, Deep-Sea Res. Pt. II, 54, 1930-1948, doi:10.1016/j.dsr2.2007.06.013, 2007.
91. Redfield, A. C., Ketchum, B. H., and Richards, F. A.: The influence of organisms on the composition of sea water, in: The Sea, edited by: Hill, M. N., Wiley-Interscience, New York, 2677, 1963.
92. Sarmiento, J. L., Gruber, N., Brzezinski, M. A., and Dunne, J. P.: High-latitude controls of thermocline nutrients and low latitude biological productivity, Nature, 427, 56-60, doi:10.1038/nature02127, 2004.
93. Sarthou, G., Vincent, D., Christaki, U., Obernosterer, I., Timmermans, K. R., and Brussaard, C. P. D.: The fate of biogenic iron during a phytoplankton bloom induced by natural fertilisation: Impact of copepod grazing, Deep-Sea Res. Pt. II, 55, 734-751, doi:10.1016/j.dsr2.2007.12.033, 2008.
94. Sarthou, G., Chever, F., Quéroué, F., Bowie, A. R., van der Merwe, P., Cheize, M., Sirois, M., and Bucciarelli, E.: Fe-Cu impact in incubation experiments of natural plankton communities and Fe-and Cu-binding ligand production at the vicinity of the Kerguelen Islands, Southern Ocean, Biogeosciences, in preparation, 2014.
95. Schlüter, M. and Rickert, D.: Effect of pH on the measurement of biogenic silica, Mar. Chem., 63, 81-92, doi:10.1016/S0304-4203(98)00052-8, 1998.
96. Smetacek, V., Klaas, C., Strass, V. H., Assmy, P., Montresor, M., Cisewski, B., Savoye, N., Webb, A., d'Ovidio, F., Arrieta, J. M., Bathmann, U., Bellerby, R., Berg, G. M., Croot, P., Gonzalez, S., Henjes, J., Herndl, G. J., Hoffmann, L. J., Leach, H., Losch, M., Mills, M. M., Neill, C., Peeken, I., Rottgers, R., Sachs, O., Sauter, E., Schmidt, M. M., Schwarz, J., Terbruggen, A., and Wolf-Gladrow, D.: Deep carbon export from a Southern Ocean iron-fertilized diatom bloom, Nature, 487, 313-319, doi:10.1038/nature11229, 2012.
97. Strickland, J. D. H. and Parsons, T. R.: A practical handbook of sea-water analysis, Second ed., Fisheries Research Board of Canada, Ottawa, Canada, 310 pp., 207-226, 1972.
98. Sunda, W. G. and Huntsman, S. A.: Interrelated influence of iron, light and cell size on marine phytoplankton growth, Nature, 390, 389-392, doi:10.1038/37093, 1997.
99. Takeda, S.: Influence of iron availability on nutrient consumption ratio of diatoms in oceanic waters, Nature, 393, 774-777, doi:10.1038/31674, 1998.
100. Tarling, G. A., Ward, P., Atkinson, A., Collins, M. A., and Murphy, E. J.: DISCOVERY 2010: Spatial and temporal variability in a dynamic polar ecosystem, Deep-Sea Res. Pt. II, 59-60, 1-13, doi:10.1016/j.dsr2.2011.10.001, 2012.
101. Tester, P. A., Geesey, M. E., Guo, C., Paerl, H. W., and Millie, D.: Evaluating phytoplankton dynamics in the Newport River Estuary (North Carolina, USA) by HPLC-derived pigment profiles, Mar. Ecol.-Prog. Ser., 124, 237-245, doi:10.3354/meps124237, 1995.
102. Timmermans, K. R., Gerringa, L. J., Baar, H. J. d., van der Wagt, B., Veldhuis, M. J., Jong, J. T. D., Croot, P. L., and Boye, M.: Growth rates and small Southern Ocean diatoms in relation to availability of iron in natural seawater, Limnol.d Oceanogr., 46, 260-266, doi:10.4319/lo.2001.46.2.0260, 2001.
103. Timmermans, K. R., Veldhuis, M. J. W., Laan, P., and Brus-saard, C. P. D.: Probing natural iron fertilization near the Ker-guelen (Southern Ocean) using natural phytoplankton assemblages and diatom cultures, Deep-Sea Res. Pt. II, 693-705, doi:10.1016/j.dsr2.2007.12.008, 2008.
104. Tréguer, P., Gueneley, S., and Kamatani, A.: Biogenic silica and particulate organic matter from the indian sector of the Southern Ocean, Mar. Chem., 23, 167-180, doi:10.1016/0304-4203(88)90030-8, 1988.
105. Trull, T. W., Davies, D., and Casciotti, K.: Insights into nutrient assimilation and export in naturally iron-fertilized waters of the Southern Ocean from nitrogen, carbon and oxygen isotopes, Deep-Sea Res. Pt. II, 55, 820-840, doi:10.1016/j.dsr2.2007.12.035, 2008.
106. Uitz, J., Claustre, H., Morel, A., and Hooker, S. B.: Vertical distribution of phytoplankton communities in open ocean: An assessment based on surface chlorophyll, J. Geophys.l Res.-Oceans, 111, C08005, doi:10.1029/2005jc003207, 2006.
107. Uitz, J., Claustre, H., Griffiths, F. B., Ras, J., Garcia, N., and San-droni, V.: A phytoplankton class-specific primary production model applied to the Kerguelen Islands region (Southern Ocean), Deep-Sea Res. Pt. I, 56, 541-560, doi:10.1016/j.dsr.2008.11.006, 2009.
108. van Beek, P., Bourquin, M., Reyss, J. L., Souhaut, M., Charette, M. A., and Jeandel, C.: Radium isotopes to investigate the water mass pathways on the Kerguelen Plateau (Southern Ocean), Deep-Sea Res. Pt. II, 55, 622-637, doi:10.1016/j.dsr2.2007.12.025, 2008.
109. van der Merwe, P., Bowie, A. R., Quéroué, F., Armand, L., Blain, S., Chever, F., Davies, D., Dehairs, F., Planchon, F., Sarthou, G., Townsend, A. T., and Trull, T.: Sourcing the iron in the naturally-fertilised bloom around the Kerguelen Plateau: particu-late trace metal dynamics, Biogeosciences Discuss., 11, 13389-13432, doi:10.5194/bgd-11-13389-2014, 2014
110. Van Heukelem, L. and Thomas, C. S.: Computer-assisted highperformance liquid chromatography method development with applications to the isolation and analysis of phytoplankton pigments, 1, Elsevier, Amsterdam, Pays-bas, 2001.
111. Vidussi, F., Claustre, H., Manca, B. B., Luchetta, A., and Marty, J.-C.: Phytoplankton pigment distribution in relation to upper thermocline circulation in the eastern Mediterranean Sea during winter, J. Geophys. Res.-Oceans, 106, 19939-19956, doi:10.1029/1999jc000308, 2001.
112. Wilks, J. V.: Diatom distribution and community composition variability on the seafloor in a naturally iron fertilised region of the Southern Ocean, Unpublished Honours Thesis, Macquarie University: North Ryde, Australia, 74 pp., 2013.
113. Wright, S. W. and Jeffrey, S. W.: Fucoxanthin pigment markers of marine phytoplankton analysed by HPLC and HPTLC, Mar. Ecol.-Prog. Ser., 38, 259-266, doi:10.3354/meps038259, 1987.
114. Wright, S. W. and van den Enden, R. L.: Phytoplankton community structure and stocks in the East Antarctic marginal ice zone (BROKE survey, January-March 1996) determined by CHEM-TAX analysis of HPLC pigment signatures, Deep-Sea Res. Pt. II, 47, 2363-2400, doi:10.1016/S0967-0645(00)00029-1, 2000.
115. Zhou, M., Zhu, Y., Dorland, R. D., and Measures, C. I.: Dynamics of the current system in the southern Drake Passage, Deep-Sea Res. Pt. I, 57, 1039-1048, doi:10.1016/j.dsr.2010.05.012, 2010.
116. Zhou, M., Zhu, Y., Measures, C. I., Hatta, M., Charette, M. A., Gille, S. T., Frants, M., Jiang, M., and Greg Mitchell, B.: Winter mesoscale circulation on the shelf slope region of the southern Drake Passage, Deep-Sea Res. Pt. II, 90, 4-14, doi:10.1016/j.dsr2.2013.03.041, 2013.
117. Zhou, M., Zhu, Y., d'Ovidio, F., Park, Y.-H., Durand, I., Kestenare, E., Sanial, V., Van-Beek, P., Queguiner, B., Carlotti, F., and Blain, S.: Surface currents and upwelling in Kerguelen Plateau regions, Biogeosciences Discuss., 11, 6845-6876, doi:10.5194/bgd-11-6845-2014, 2014.