Non-specific interaction of carbon nanotubes with the resazurin assay reagent: Impact on in vitro assessment of nanoparticle cytotoxicity

Toxicology in Vitro

Volumn 29, Issue 1, 2015, Pages 142-147

  Breznan, Dalibor ^a   Das, Dharani ^a   MacKinnon Roy, Christine ^a   Simard, Benoit ^b   Kumarathasan, Premkumari ^a   Vincent, Renaud ^a  

^a HEALTH CANADA   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Carbon nanotubes; Cell viability; Cytotoxicity; In vitro; Interaction; Resazurin

Indexed keywords

CARBON NANOTUBE; MULTI WALLED NANOTUBE; RESAZURIN; RESORUFIN; SINGLE WALLED NANOTUBE; NANOPARTICLE; OXAZINE DERIVATIVE; XANTHENE DERIVATIVE;

ANIMAL CELL; ARTICLE; ARTIFACT; CELL CULTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; CYTOTOXICITY ASSAY; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR INTERACTION; MOLECULAR STABILITY; NONHUMAN; CELL LINE; CELL SURVIVAL; DIAGNOSTIC USE; DRUG EFFECTS; FLUORESCENCE; PROCEDURES; TOXICITY TESTING;

CELL LINE; CELL SURVIVAL; FLUORESCENCE; HUMANS; NANOPARTICLES; NANOTUBES, CARBON; OXAZINES; TOXICITY TESTS; XANTHENES;

EID: 84908377000     PISSN: 08872333     EISSN: 18793177     Source Type: Journal    
DOI: 10.1016/j.tiv.2014.09.009     Document Type: Article

References (24)

1. Casey A., Herzog E., Davoren M., Lyng F.M., Byrne H.J., Chambers G. Spectroscopic analysis confirms the interactions between single walled carbon nanotubes and various dyes commonly used to assess cytotoxicity. Carbon 2007, 45:1425-1432.
2. Chiu C.F., Dementev N., Borguet E. Fluorescence quenching of dyes covalently attached to single-walled carbon nanotubes. J. Phys. Chem. A 2011, 115:9579-9584.
3. De Fries F.R., Mitsuhashi M. Quantification of mitogen induced human lymphocyte proliferation: comparison of Alamar Blue assay to 3H-thymidine incorporation assay. J. Clin. Lab. Anal. 1995, 9:89-95.
4. De Jong D.W., Woodlief W.G. Fluorimetric assay of tobacco leaf dehydrogenases with resazurin. Biochim. Biophys. Acta 1977, 484:249-259.
5. Doak S.H., Griffiths S.M., Manshian B., Singh N., Williams P.M., Brown A.P., Jenkins G.J. Confounding experimental considerations in nanogenotoxicology. Mutagenesis 2009, 24:285-293.
6. Geraci C.L., Castranova V. Challenges in assessing nanomaterial toxicology: a personal perspective. Wiley. Interdiscip. Rev. Nanomed. Nanobiotechnol. 2010, 2:569-577.
7. Goegan P., Johnson G., Vincent R. Effects of serum protein and colloid on the alamarBlue assay in cell cultures. Toxicol. In Vitro 1995, 9:257-266.
8. Gonzalez R.J., Tarloff J.B. Evaluation of hepatic subcellular fractions for Alamar blue and MTT reductase activity. Toxicol. In Vitro 2001, 15:257-259.
9. Isobe H., Tanaka T., Maeda R., Noiri E., Solin N., Yudasaka M., Iijima S., Nakamura E. Preparation, purification, characterization, and cytotoxicity assessment of water-soluble, transition-metal-free carbon nanotube aggregates. Angew. Chem. Int. Ed. Engl. 2006, 45:6676-6680.
10. Kingston C.T., Jakubek Z.J., Dénommée S., Simard B. Efficient laser synthesis of single-walled carbon nanotubes through laser heating of the condensing vaporization plume. Carbon 2004, 42:1657-1664.
11. Kroll A., Pillukat M.H., Hahn D., Schnekenburger J. Current in vitro methods in nanoparticle risk assessment: limitations and challenges. Eur. J. Pharm. Biopharm. 2009, 72:370-377.
12. Kroll A., Pillukat M.H., Hahn D., Schnekenburger J. Interference of engineered nanoparticles with in vitro toxicity assays. Arch. Toxicol. 2012, 86(7):1123-1136.
13. Kumarathasan P., Das D., Salam M.A., Mohottalage S., DeSilva N., Simard B., Vincent R. Mass spectrometry-based proteomic assessment of the in vitro toxicity of carbon nanotubes. Curr. Top. Biochem. Res. 2012, 14(1):15-27.
14. Kumarathasan P., Breznan D., Das D., Salam M.A., Siddiqui Y., MacKinnon-Roy C., Jingwen G., De Silva N., Simard B., Vincent R. Cytotoxicity of carbon nanotube variants: a comparative in vitro exposure study with A549 epithelial and J774 macrophage cells. Nanotoxicology 2014, [Epub ahead of print] PubMed PMID: 24713075.
15. Monteiro-Riviere N.A., Inman A.O., Zhang L.W. Limitations and relative utility of screening assays to assess engineered nanoparticle toxicity in a human cell line. Toxicol. Appl. Pharmacol. 2009, 234:222-235.
16. Nadeau D., Vincent R., Kumarathasan P., Brook J., Dufresne A. Cytotoxicity of ambient air particles to rat lung macrophages: comparison of cellular and functional assays. Toxicol. In Vitro 1996, 10:161-172.
17. O'Brien J., Wilson I., Orton T., Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 2000, 267:5421-5426.
18. Ong K.J., Maccormack T.J., Clark R.J., Ede J.D., Ortega V.A., Felix L.C., Dang M.K., Ma G., Fenniri H., Veinot J.G., Goss G.G. Widespread nanoparticle-assay interference: implications for nanotoxicity testing. PLoS ONE 2014, 9(3):e90650.
19. Oostingh G.J., Casals E., Italiani P., Colognato R., Stritzinger R., Ponti J., Pfaller T., Kohl Y., Ooms D., Favilli F., Leppens H., Lucchesi D., Rossi F., Nelissen I., Thielecke H., Puntes V.F., Duschl A., Boraschi D. Problems and challenges in the development and validation of human cell-based assays to determine nanoparticle-induced immunomodulatory effects. Part Fibre. Toxicol. 2011, 8:8.
20. Riss T.L., Moravec R.A. Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. Assay Drug Dev. Technol. 2004, 2:51-62.
21. Rodriguez-Yanez Y., Munoz B., Albores A. Mechanisms of toxicity of carbon nanotubes. Toxicol. Mech. Methods 2012, 23(3):178-195.
22. Vincent R., Goegan P., Johnson G., Brook J.R., Kumarathasan P., Bouthillier L., Burnett R.T. Regulation of promoter-CAT stress genes in HepG2 cells by suspensions of particles from ambient air. Fundam. Appl. Toxicol. 1997, 39:18-32.
23. Worle-Knirsch J.M., Pulskamp K., Krug H.F. Oops they did it again! Carbon nanotubes hoax scientists in viability assays. Nano Lett. 2006, 6:1261-1268.
24. Yang K., Liu Z. In vivo biodistribution, pharmacokinetics, and toxicology of carbon nanotubes. Curr. Drug Metab. 2012, 13:1057-1067.