A-T linker adapter polymerase chain reaction for determining flanking sequences by rescuing inverse PCR or thermal asymmetric interlaced PCR products

Analytical Biochemistry

Volumn 466, Issue , 2014, Pages 24-26

  Trinh, Quoclinh ^a   Zhu, Pengyu ^a   Shi, Hui ^a   Xu, Wentao ^a   Hao, Junran ^a   Luo, Yunbo ^a,b   Huang, Kunlun ^a,b  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b AGRO ENVIRONMENTAL PROTECTION INSTITUTE   (China)

Author keywords

A T linker adapter PCR; Flanking sequence; Genome walking; PCR product rescue

Indexed keywords

CLONE CELLS; DNA SEQUENCES; GENE ENCODING; INVERSE PROBLEMS;

A-T LINKER ADAPTER PCR; ARABIDOPSIS MUTANT; FLANKING SEQUENCE; GENOME-WALKING; GENOMIC DNA; INVERSE PCR; PCR PRODUCTS;

POLYMERASE CHAIN REACTION;

DNA POLYMERASE; GENOMIC DNA; MESSENGER RNA; OLIGONUCLEOTIDE; PAPAIN; RESTRICTION ENDONUCLEASE;

5' UNTRANSLATED REGION; A T LINKER ADAPTER POLYMERASE CHAIN REACTION; ARABIDOPSIS; ARTICLE; CHROMOSOME WALKING; DNA FLANKING REGION; DNA TEMPLATE; DNA TRANSFER; GENE INSERTION; GENE SEQUENCE; INTRON; INVERSE POLYMERASE CHAIN REACTION; MOLECULAR CLONING; NONHUMAN; PAPAYA; PLANT GENE; PLANT GENOME; POLYMERASE CHAIN REACTION; PROMOTER REGION; RESTRICTION SITE; THERMAL ASYMMETRIC INTERLACED POLYMERASE CHAIN REACTION; DNA SEQUENCE; GENETICS; PROCEDURES;

5' FLANKING REGION; CHROMOSOME WALKING; PAPAIN; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; SEQUENCE ANALYSIS, DNA;

EID: 84908218895     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.07.022     Document Type: Article

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