Tropism and replication of Middle East respiratory syndrome coronavirus from dromedary camels in the human respiratory tract: An in-vitro and ex-vivo study

The Lancet Respiratory Medicine

Volumn 2, Issue 10, 2014, Pages 813-822

  Chan, Renee W Y ^a,b   Hemida, Maged G ^c,d   Kayali, Ghazi ^e   Chu, Daniel K W ^a,b   Poon, Leo L M ^a,b   Alnaeem, Abdelmohsen ^c   Ali, Mohamed A ^f   Tao, Kin P ^a,b   Ng, Hoi Y ^a,b   Chan, Michael C W ^a,b   Guan, Yi ^a,b   Nicholls, John M ^a   Peiris, J S Malik ^a,b  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

^c KING FAISAL UNIVERSITY   (Saudi Arabia)

^d Kafrelsheikh University   (Egypt)

^e St Jude Children's Rsch Hosp   (United States)

^f NATIONAL RESEARCH CENTRE   (Egypt)

Author keywords

[No Author keywords available]

Indexed keywords

GAMMA INTERFERON INDUCIBLE PROTEIN 10; INTERLEUKIN 6; TUMOR NECROSIS FACTOR ALPHA; VIRUS RNA;

ANIMAL CELL; ARTICLE; BRONCHUS; CONTROLLED STUDY; CORONAVIRUS; CYTOKINE PRODUCTION; CYTOKINE RESPONSE; DROMEDARY; EGYPT; EX VIVO STUDY; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNE EVASION; IN VITRO STUDY; INFLUENZA VIRUS A H5N1; LUNG; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; NONHUMAN; SAUDI ARABIA; STRAIN DIFFERENCE; VIRAL TROPISM; VIRUS INFECTIVITY; VIRUS ISOLATION; VIRUS REPLICATION; VIRUS STRAIN; ANIMAL; CAMEL; CORONAVIRUS INFECTION; PATHOGENICITY; PHYSIOLOGY; RESPIRATORY SYSTEM; VIROLOGY;

ANIMALS; CAMELS; CORONAVIRUS INFECTIONS; EGYPT; HUMANS; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; RESPIRATORY SYSTEM; SAUDI ARABIA; VIRAL TROPISM; VIRUS REPLICATION;

EID: 84908030055     PISSN: 22132600     EISSN: 22132619     Source Type: Journal    
DOI: 10.1016/S2213-2600(14)70158-4     Document Type: Article

References (22)

1. Middle East respiratory syndrome coronavirus (MERS-CoV)-update WHO, (accessed Aug 12, 2014). http://www.who.int/csr/don/2014_07_23_mers/en.
2. Middle East respiratory syndrome coronavirus (MERS-CoV) summary and literature update WHO, (accessed July 9, 2014). http://www.who.int/csr/disease/coronavirus_infections/MERS-CoV_summary_update_20140611.pdf.
3. Middle East respiratory syndrome coronavirus (MERS-CoV) WHO, (accessed Aug 23, 2014). http://www.who.int/csr/disease/coronavirus_infections/MERS_CoV_RA_20140424.pdf?ua=1.
4. Cauchemez S, Fraser C, Van Kerkhove MD, et al. Middle East respiratory syndrome coronavirus: quantification of the extent of the epidemic, surveillance biases, and transmissibility. Lancet Infect Dis 2014, 14:50-56.
5. Reusken CB, Haagmans BL, Muller MA, et al. Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study. Lancet Infect Dis 2013, 13:859-866.
6. Perera RA, Wang P, Gomaa MR, et al. Seroepidemiology for MERS coronavirus using microneutralisation and pseudoparticle virus neutralisation assays reveal a high prevalence of antibody in dromedary camels in Egypt, June 2013. Euro Surveill 2013, 18:20574.
7. Haagmans BL, Al Dhahiry SH, Reusken CB, et al. Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation. Lancet Infect Dis 2014, 14:140-145.
8. Alagaili AN, Briese T, Mishra N, et al. Middle East respiratory syndrome coronavirus infection in dromedary camels in Saudi Arabia. MBio 2014, 5:e00884. 14.
9. Azhar EI, El-Kafrawy SA, Farraj SA, et al. Evidence for camel-to-human transmission of MERS coronavirus. N Engl J Med 2014, 370:2499-2505.
10. Cotten M, Watson SJ, Zumla AI, et al. Spread, circulation, and evolution of the Middle East respiratory syndrome coronavirus. MBio 2014, 5:e01062. 13.
11. Chu DKW, Poon LLM, Gomaa MM, et al. MERS coronaviruses in dromedary camels, Egypt. Emerg Infect Dis 2014, 20:149-153.
12. Reusken CBEM, Messadi L, Feyisa A, et al. Geographic distribution of MERS coronavirus among dromedary camels, Africa. Emerg Infect Dis 2014, 20:1370-1374.
13. Aburizaiza AS, Mattes FM, Azhar EI, et al. Investigation of anti-middle East respiratory syndrome antibodies in blood donors and slaughterhouse workers in Jeddah and Makkah, Saudi Arabia, fall 2012. J Infect Dis 2014, 209:243-246.
14. Hemida MG, Chu DKW, Poon LLM, et al. MERS coronavirus in dromedary camel herd, Saudi Arabia. Emerg Infect Dis 2014, 20:1231-1234.
15. Holmes KV Structural biology: adaptation of SARS coronavirus to humans. Science 2005, 309:1822-1823.
16. Stalin Raj V, Farag EABA, Reusken CBEM, et al. Isolation of MERS coronavirus from dromedary camel, Qatar, 2014. Emerg Infect Dis 2014, 20:1339-1342.
17. Chan RW, Chan MC, Agnihothram S, et al. Tropism of and innate immune responses to the novel human betacoronavirus lineage C virus in human ex vivo respiratory organ cultures. J Virol 2013, 87:6604-6614.
18. Briese T, Mishra N, Jain K, et al. Middle East respiratory syndrome coronavirus quasispecies that include homologues of human isolates revealed through whole-genome analysis and virus cultured from dromedary camels in Saudi Arabia. MBio 2014, 5:e01146. 14.
19. Kindler E, Jonsdottir HR, Muth D, et al. Efficient replication of the novel human betacoronavirus EMC on primary human epithelium highlights its zoonotic potential. MBio 2013, 4:e00611. 12.
20. Harcourt JL, Caidi H, Anderson LJ, et al. Evaluation of the Calu-3 cell line as a model of in vitro respiratory syncytial virus infection. J Virol Methods 2011, 174:144-149.
21. Peiris JS, de Jong MD, Guan Y Avian influenza virus (H5N1): a threat to human health. Clin Microbiol Rev 2007, 20:243-267.
22. van Doremalen N, Bushmaker T, Karesh WB, Munster VJ Stability of Middle East respiratory syndrome coronavirus in milk. Emerg Infect Dis 2014, 20:1263-1264.