Oxidative refolding from inclusion bodies

Methods in Molecular Biology

Volumn 1140, Issue , 2014, Pages 145-157

  Nelson, Christopher A ^a   Lee, Chung A ^a   Fremont, Daved H ^a  

^a NONE

Author keywords

Aggregation; Cell lysis; Escherichia coli; Feedback inhibition; Inclusion bodies; Induction; Protein concentration; Protein purification; Protein refolding; Recombinant protein expression; Selenomethionine labeling; Size exclusion chromatography; Solubilization

Indexed keywords

ARGININE; BACTERIAL PROTEIN; DISULFIDE; GLUTATHIONE; METHIONINE; RECOMBINANT PROTEIN; SELENOMETHIONINE; PROTEIN;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; CELL INCLUSION; DILUTION; ESCHERICHIA COLI; NEGATIVE FEEDBACK; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN PURIFICATION; PROTEIN REFOLDING; PROTEIN TARGETING; REDUCTION; RENATURATION; SIZE EXCLUSION CHROMATOGRAPHY; SOLUBILIZATION; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR BIOLOGY; OXIDATION REDUCTION REACTION; PROTEIN FOLDING;

ESCHERICHIA COLI; INCLUSION BODIES; MOLECULAR BIOLOGY; OXIDATION-REDUCTION; PROTEIN FOLDING; PROTEINS;

EID: 84907941366     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0354-2_11     Document Type: Article

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