Single-cell raman sorting

Methods in Molecular Biology

Volumn 1096, Issue , 2014, Pages 147-153

  Li, Mengqiu ^a   Boardman, Dan G ^a   Ward, Andrew ^b   Huang, Wei E ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b Science and Technology Facilities Council   (United Kingdom)

Author keywords

Raman; Raman sorting; Single cell; Stable isotope probing; Unculturable microorganisms

Indexed keywords

RIBOSOME RNA; STABLE ISOTOPE;

ARTICLE; BACTERIAL CELL; BACTERIAL GENETICS; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CARBON UTILIZATION; CELL DIFFERENTIATION; CELL SELECTION; CELL SUSPENSION; CELL TYPE; CHEMICAL ANALYSIS; CONTROLLED STUDY; ECOSYSTEM; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE FUNCTION; GENE SEQUENCE; NONHUMAN; NUTRIENT; OPTICAL TWEEZERS; PHENOTYPE; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS; RAMAN SPECTROMETRY; SEQUENCE ANALYSIS; SINGLE CELL RAMAN SPECTROSCOPY; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; SINGLE CELL ANALYSIS; BACTERIAL GENOME; BACTERIUM CULTURE; CELL ISOLATION; NON INVASIVE PROCEDURE; TECHNOLOGY;

MICROBIOLOGICAL TECHNIQUES; SINGLE-CELL ANALYSIS; SPECTRUM ANALYSIS, RAMAN;

EID: 84907676502     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-712-9_12     Document Type: Article

References (16)

1. Wagner M et al (1993) Probing activated-sludge with oligonucleotides specifi c for proteobacteria - inadequacy of culture-dependent methods for describing microbial community structure. Appl Environ Microbiol 59: 1520-1525 (Pubitemid 23141165)
2. Riesenfeld CS et al (2004) Metagenomics: genomic analysis of microbial communities. Annu Rev Genet 38:525-552 (Pubitemid 40013737)
3. Jones JG (1977) Effect of environmentalfactors on estimated viable and total populations of planktonic bacteria in lakes and experimental enclosures. Freshwater Biol 7: 67-91
4. Ferguson RL et al (1984) Response of marine bacterioplankton to differential fi ltration and confi nement. Appl Environ Microbiol 47:49-55
5. Torsvik V et al (1990) High diversity in DNA of soil bacteria. Appl Environ Microbiol 56: 782-787 (Pubitemid 20088454)
6. Ellis RJ et al (2003) Cultivation-dependent and-independent approaches for determining bacterial diversity in heavy-metal-contaminated soil. Appl Environ Microbiol 69:3223-3230 (Pubitemid 36693325)
7. Rappe MS, Giovannoni SJ (2003) The uncultured microbial majority. Annu Rev Microbiol 57:369-394 (Pubitemid 37392948)
8. Huang WE et al (2010) Shining light on the microbial world: the application of Raman microspectroscopy. Adv Appl Microbiol 70: 153-186
9. Huang WE et al (2009) Resolving genetic functions within microbial populations: in situ analyses using rRNA and mRNA stable isotope probing coupled with single-cell Ramanfl uorescence in situ hybridization. Appl Environ Microbiol 75:234-241
10. Huang WE et al (2004) Raman microscopic analysis of single microbial cells. Anal Chem 76:4452-4458 (Pubitemid 39014020)
11. Huang WE et al (2007) Raman-FISH: combining stable-isotope Raman spectroscopy and fl uorescence in situ hybridization for the single cell analysis of identity and function. Environ Microbiol 9:1878-1889 (Pubitemid 47057364)
12. Huang WE et al (2009) Raman tweezers sorting of single microbial cells. Environ Microbiol Rep 1:44-49
13. Liberal ATD et al (2005) Contaminant yeast detection in industrial ethanol fermentation must by rDNA-PCR. Lett Appl Microbiol 40: 19-23
14. Marchesi JR et al (1998) Design and evaluation of useful bacterium-specifi c PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64: 795-799 (Pubitemid 28079862)
15. Huang WE et al (2005) Chromosomally located gene fusions constructed in Acinetobacter sp ADP1 for the detection of salicylate. Environ Microbiol 7:1339-1348 (Pubitemid 41204928)
16. Delorenzo V et al (1990) Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J Bacteriol 172:6568-6572