Slide preparation method to preserve three-dimensional chromatin architecture of testicular germ cells

Journal of Visualized Experiments

Volumn , Issue 83, 2014, Pages

  Namekawa, Satoshi H ^a,b  

^a CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER   (United States)

^b UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

Basic Protocol; Chromatin; Germ cells; Issue 83; Meiotic sex chromosome inactivation; Postmeiotic sex chromatin; Sex chromosomes; Testis

Indexed keywords

EID: 84907652234     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50819     Document Type: Article

References (10)

1. Peters, A. H., Plug, A. W., van Vugt, M. J. & de Boer, P. A drying-down technique for the spreading of mammalian meiocytes from the male and female germline. Chromosome Res. 5, 66-68 (1997).
2. Namekawa, S. H. et al. Postmeiotic sex chromatin in the male germline of mice. Curr. Biol. 16, 660-667, doi:S0960-9822(06)01278-4 [pii]10.1016/j.cub.2006.01.066 (2006).
3. Namekawa, S. H. & Lee, J. T. Detection of nascent RNA, single-copy DNA and protein localization by immunoFISH in mouse germ cells and preimplantation embryos. Nat. Protoc. 6, 270-284, doi:nprot.2010.195 [pii]10.1038/nprot.2010.195 (2011).
4. Mahadevaiah, S. K., Costa, Y. & Turner, J. M. Using RNA FISH to study gene expression during mammalian meiosis. Methods Mol. Biol. 558, 433-444, doi:10.1007/978-1-60761-103-5_25 (2009).
5. Hall, L. L. et al. An ectopic human XIST gene can induce chromosome inactivation in postdifferentiation human HT-1080 cells. Proc. Natl. Acad. Sci. U.S.A. 99, 8677-8682, doi:10.1073/pnas.132468999132468999 [pii] (2002).
6. Huynh, K. D. & Lee, J. T. Inheritance of a pre-inactivated paternal X chromosome in early mouse embryos. Nature. 426, 857-862, doi:10.1038/nature02222nature02222 [pii] (2003).
7. Namekawa, S. H., VandeBerg, J. L., McCarrey, J. R. & Lee, J. T. Sex chromosome silencing in the marsupial male germ line. Proc. Natl. Acad. Sci. U.S.A. 104, 9730-9735, doi:10.1073/pnas.0700323104 (2007).
8. Namekawa, S. H., Payer, B., Huynh, K. D., Jaenisch, R. & Lee, J. T. Two-step imprinted X inactivation: repeat versus genic silencing in the mouse. Mol. Cell Biol. 30, 3187-3205, doi:10.1128/MCB.00227-10MCB.00227-10 [pii] (2010).
9. Ichijima, Y. et al. MDC1 directs chromosome-wide silencing of the sex chromosomes in male germ cells. Genes Dev. 25, 959-971, doi:10.1101/gad.203081125/9/959 [pii] (2011).
10. Sin, H. S. et al. RNF8 regulates active epigenetic modifications and escape gene activation from inactive sex chromosomes in post-meiotic spermatids. Genes Dev. 26, 2737-2748, doi:10.1101/gad.202713.11226/24/2737 [pii] (2012).