Quantitative PCR for detection of mRNA and gDNA in environmental isolates

Methods in Molecular Biology

Volumn 1096, Issue , 2014, Pages 25-42

  Brzoska, Anthony J ^a   Hassan, Karl A ^b  

^a UNIVERSITY OF SYDNEY   (Australia)

^b MACQUARIE UNIVERSITY   (Australia)

Author keywords

Amplification efficiency; PCR; Quantitative; Reverse transcription

Indexed keywords

COMPLEMENTARY DNA; GENOMIC DNA; MESSENGER RNA; SIDEROPHORE; DNA; NUCLEIC ACID; RNA;

ACINETOBACTER BAUMANNII; ARTICLE; BACTERIUM ISOLATE; METHODOLOGY; NONHUMAN; NUCLEIC ACID ANALYSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RELIABILITY; CONTROLLED STUDY; DATA ANALYSIS; DNA EXTRACTION; DNA MARKER; DNA PURIFICATION; DNA SYNTHESIS; GENE EXPRESSION; GENETIC MARKER; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PURIFICATION; MICROBIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; STANDARD;

DNA; ENVIRONMENTAL MICROBIOLOGY; GENETIC MARKERS; REAL-TIME POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 84907624019     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-712-9_3     Document Type: Article

References (13)

1. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611-622
2. VanGuilder HD, Vrana KE, Freeman WM (2008) Twenty-five years of quantitative PCR for gene expression analysis. Biotechniques 44:619-626 (Pubitemid 351656280)
3. Higuchi R, Fockler C, Dollinger G, Watson R (1993) Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology 11:1026-1030 (Pubitemid 23254023)
4. Holland PM, Abramson RD, Watson R, Gelfand DH (1991) Detection of specific polymerase chain reaction product by utilizing the 5-3 exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci U S A 88:7276-7280 (Pubitemid 21915133)
5. Tyagi S, Kramer FR (1996) Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol 14:303-308
6. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365-386
7. You FM, Huo N, Gu YQ, Luo MC, Ma Y, Hane D, Lazo GR, Dvorak J, Anderson OD (2008) BatchPrimer3: a high throughput web application for PCR and sequencing primer design. BMC Bioinformatics 9:253
8. Brzoska AJ, Hassan KA, de Leon EJ, Paulsen IT, Lewis PJ (2013) Single-step selection of drug resistant Acinetobacter baylyi ADP1 mutants reveals a functional redundancy in the recruitment of multidrug efflux systems. PLoS ONE 8:e56090
9. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402-408 (Pubitemid 34164012)
10. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45
11. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159 (Pubitemid 17064313)
12. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
13. Hassan KA, Brzoska AJ, Wilson NL, Eijkelkamp BA, Brown MH, Paulsen IT (2011) Roles of DHA2 family transporters in drug resistance and iron homeostasis in Acinetobacter spp. J Mol Microbiol Biotechnol 20:116-124