A widely used in vitro biofilm assay has questionable clinical significance for enterococcal endocarditis

PLoS ONE

Volumn 9, Issue 9, 2014, Pages

  Leuck, Anne Marie ^a   Johnson, James R ^a,b   Dunny, Gary M ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL TISSUE; ARTICLE; BACTERIAL ENDOCARDITIS; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATE; BIOASSAY; BIOFILM; COLONY FORMING UNIT; CONTROLLED STUDY; ENTEROCOCCAL INFECTION; ENTEROCOCCUS FAECALIS; EX VIVO STUDY; HEART VALVE ASSAY; INCUBATION TIME; INTERMETHOD COMPARISON; NONHUMAN; POLYSTYRENE DISH ASSAY; QUANTITATIVE ANALYSIS; SWINE; ANIMAL; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECTS; ENDOCARDITIS; GROWTH, DEVELOPMENT AND AGING; HEART VALVE; MICROBIOLOGY; PHYSIOLOGY; PIG; PROCEDURES; SPECIES DIFFERENCE;

ANIMALIA; ENTEROCOCCUS FAECALIS; SUS;

GLUCOSE; POLYSTYRENE DERIVATIVE;

ANIMALS; BIOFILMS; BIOLOGICAL ASSAY; CULTURE TECHNIQUES; ENDOCARDITIS; ENTEROCOCCUS FAECALIS; GLUCOSE; HEART VALVES; POLYSTYRENES; SPECIES SPECIFICITY; SWINE;

EID: 84907584434     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0107282     Document Type: Article

References (28)

1. Potera C (1999) Forging a link between biofilms and disease. Science 283: 1837-1839.
2. Research on Microbial Biofilms, NIH Parent Grant Announcement. 2002. NIH website. Available: http://grants.nih.gov/grants/guide/pa-files/PA-03-047.html. Accessed 2014 Sept 2.
3. Ostrolenk M, Hunter AC (1946) The Distribution of Enteric Streptococci. J Bacteriol 51: 735-741.
4. Murdoch DR, Corey GR, Hoen B, Miro JM, Fowler VG Jr, et al. (2009) Clinical presentation, etiology, and outcome of infective endocarditis in the 21st century: the International Collaboration on Endocarditis-Prospective Cohort Study. Arch Intern Med 169: 463-473.
5. Paganelli FL, Willems RJ, Leavis HL (2012) Optimizing future treatment of enterococcal infections: attacking the biofilm? Trends Microbiol 20: 40-49.
6. Baldassarri L, Simpson WA, Donelli G, Christensen GD (1993) Variable fixation of staphylococcal slime by different histochemical fixatives. Eur J Clin Microbiol Infect Dis 12: 866-868.
7. Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, et al. (1985) Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol 22: 996-1006.
8. Kristich CJ, Li YH, Cvitkovitch DG, Dunny GM (2004) Esp-independent biofilm formation by Enterococcus faecalis. J Bacteriol 186: 154-163.
9. Tendolkar PM, Baghdayan AS, Gilmore MS, Shankar N (2004) Enterococcal surface protein, Esp, enhances biofilm formation by Enterococcus faecalis. Infect Immun 72: 6032-6039.
10. Gao P, Pinkston KL, Nallapareddy SR, van Hoof A, Murray BE, et al. (2010) Enterococcus faecalis rnjB is required for pilin gene expression and biofilm formation. J Bacteriol 192: 5489-5498.
11. Toledo-Arana A, Valle J, Solano C, Arrizubieta MJ, Cucarella C, et al. (2001) The enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formation. Appl Environ Microbiol 67: 4538-4545.
12. Lopez-Salas P, Llaca-Diaz J, Morfin-Otero R, Tinoco JC, Rodriguez-Noriega E, et al. (2013) Virulence and antibiotic resistance of Enterococcus faecalis clinical isolates recovered from three states of Mexico. Detection of linezolid resistance. Arch Med Res 44: 422-428.
13. Schlievert PM, Gahr PJ, Assimacopoulos AP, Dinges MM, Stoehr JA, et al. (1998) Aggregation and binding substances enhance pathogenicity in rabbit models of Enterococcus faecalis endocarditis. Infect Immun 66: 218-223.
14. Chuang-Smith ON, Wells CL, Henry-Stanley MJ, Dunny GM (2010) Acceleration of Enterococcus faecalis biofilm formation by aggregation substance expression in an ex vivo model of cardiac valve colonization. PLoS One 5: e15798.
15. Frank KL, Guiton PS, Barnes AM, Manias DA, Chuang-Smith ON, et al. (2013) AhrC and Eep are biofilm infection-associated virulence factors in Enterococcus faecalis. Infect Immun 81: 1696-1708.
16. Shankar V, Baghdayan AS, Huycke MM, Lindahl G, Gilmore MS (1999) Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface protein. Infect Immun 67: 193-200.
17. Udo EE, Al-Sweih N (2011) Frequency of virulence-associated genes in Enterococccus faecalis isolated in Kuwait hospitals. Med Princ Pract 20: 259-264.
18. Dunny GM, Brown BL, Clewell DB (1978) Induced cell aggregation and mating in Streptococcus faecalis: evidence for a bacterial sex pheromone. Proc Natl Acad Sci U S A 75: 3479-3483.
19. Kristich CJ, Nguyen VT, Le T, Barnes AM, Grindle S, et al. (2008) Development and use of an efficient system for random mariner transposon mutagenesis to identify novel genetic determinants of biofilm formation in the core Enterococcus faecalis genome. Appl Environ Microbiol 74: 3377-3386.
20. Baldassarri L, Cecchini R, Bertuccini L, Ammendolia MG, Iosi F, et al. (2001) Enterococcus spp. produces slime and survives in rat peritoneal macrophages. Med Microbiol Immunol 190: 113-120.
21. McBride SM, Fischetti VA, Leblanc DJ, Moellering RC Jr, Gilmore MS (2007) Genetic diversity among Enterococcus faecalis. PLoS One 2: e582.
22. Huycke MM, Gilmore MS (1995) Frequency of aggregation substance and cytolysin genes among enterococcal endocarditis isolates. Plasmid 34: 152-156.
23. Shankar N, Lockatell CV, Baghdayan AS, Drachenberg C, Gilmore MS, et al. (2001) Role of Enterococcus faecalis surface protein Esp in the pathogenesis of ascending urinary tract infection. Infect Immun 69: 4366-4372.
24. Frank KL, Barnes AM, Grindle SM, Manias DA, Schlievert PM, et al. (2012) Use of recombinase-based in vivo expression technology to characterize Enterococcus faecalis gene expression during infection identifies in vivo-expressed antisense RNAs and implicates the protease Eep in pathogenesis. Infect Immun 80: 539-549.
25. Sandoe JA, Wysome J, West AP, Heritage J, Wilcox MH (2006) Measurement of ampicillin, vancomycin, linezolid and gentamicin activity against enterococcal biofilms. J Antimicrob Chemother 57: 767-770.
26. Lebeaux D, Chauhan A, Rendueles O, Beloin C (2013) From in vitro to in vivo Models of Bacterial Biofilm-Related Infections. Pathogens 2: 288-365.
27. Lizcano A, Chin T, Sauer K, Tuomanen EI, Orihuela CJ (2010) Early biofilm formation on microtiter plates is not correlated with the invasive disease potential of Streptococcus pneumoniae. Microb Pathog 48: 124-130.
28. Sandoe JA, Witherden IR, Cove JH, Heritage J, Wilcox MH (2003) Correlation between enterococcal biofilm formation in vitro and medical-device-related infection potential in vivo. J Med Microbiol 52: 547-550.