Conjugation of proteins to polymer chains to create multivalent molecules

Methods in Molecular Biology

Volumn 1202, Issue , 2014, Pages 95-102

  Conway, Anthony ^a   Spelke, Dawn P ^a   Schaffer, David V ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Biopolymers; Conjugation; Multivalency; Stem cells

Indexed keywords

CELL RECEPTOR; LIGAND; POLYMER; RECOMBINANT PROTEIN;

ARTICLE; CELL FUNCTION; CONJUGATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; STEM CELL; SYNTHESIS;

EID: 84907359868     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/7651-2013-32     Document Type: Article

References (15)

1. Mammen M, Choi S-K, Whitesides GM (1998) Polyvalent interactions in biological systems: implications for design and use of multivalent ligands and inhibitors. Angew Chem Int Ed 37:2754-2794
2. Kiessling LL, Gestwicki JE, Strong LE (2000) Synthetic multivalent ligands in the exploration of cell-surface interactions. Curr Opin Chem Biol 4:696-703
3. Artavanis-Tsakonas S, Rand MD, Lake RJ (1999) Notch signaling: cell fate control and signal integration in development. Science 284:770-776
4. Ashton RS, Conway A, Pangarkar C, Bergen J, Lim KI, Shah P, Bissell M, Schaffer DV (2012) Astrocytes regulate adult hippocampal neurogenesis through ephrin-B signaling. Nat Neurosci 15:1399-1406
5. Vyas N, Goswami D, Manonmani A, Sharma P, Ranganath HA, VijayRaghavan K, Shashidhara LS, Sowdhamini R, Mayor S (2008) Nanoscale organization of hedgehog is essential for longrange signaling. Cell 133:1214-1227
6. Ye S, Luo Y, Lu W, Jones RB, Linhardt RJ, Capila I, Toida T, Kan M, Pelletier H, McKeehan WL (2001) Structural basis for interaction of FGF-1, FGF-2, and FGF-7 with different heparan sulfate motifs. Biochemistry 40:14429-14439
7. Krilleke D, Ng YS, Shima DT (2009) The heparin-binding domain confers diverse functions of VEGF-A in development and disease: a structure-function study. Biochem Soc Trans 37:1201-1206
8. Davis S, Gale NW, Aldrich TH, Maisonpierre PC, Lhotak V, Pawson T, Goldfarb M, Yancopoulos GD (1994) Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity. Science 266:816-819
9. Arlaud GJ, Colomb MG, Gagnon J (1987) A functional-model of the human C1 complex-emergence of a functional-model. Immunol Today 8:106-111
10. Wall ST, Saha K, Ashton RS, Kam KR, Schaffer DV, Healy KE (2008) Multivalency of Sonic hedgehog conjugated to linear polymer chains modulates protein potency. Bioconjug Chem 19:806-812
11. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning : a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
12. Zachariou M (2008) Affinity chromatography: methods and protocols. Humana, Totowa, NJ
13. Hames BD (1998) Gel electrophoresis of proteins : a practical approach. Oxford University Press, Oxford
14. Yu Y, DesLauriers PJ, Rohlfing DC (2005) SEC-MALS method for the determination of long-chain branching and long-chain branching distribution in polyethylene. Polymer 46:5165-5182
15. Pollock JF, Ashton RS, Rode NA, Schaffer DV, Healy KE (2012) Molecular characterization of multivalent bioconjugates by size-exclusion chromatography with multiangle laser light scattering. Bioconjug Chem 23:1794-1801