Reconstruction of normal and pathological human epidermis on polycarbonate filter

Methods in Molecular Biology

Volumn 1195, Issue , 2014, Pages 191-201

  De Vuyst, Evelyne ^a   Charlier, Céline ^a   Giltaire, Séverine ^a   De Glas, Valérie ^a   De Rouvroit, Catherine Lambert ^a   Poumay, Yves ^a  

^a UNIVERSITY OF NAMUR   (Belgium)

Author keywords

Darier's disease; Epidermis reconstruction; Human keratinocytes; Primary culture; Skin biopsy

Indexed keywords

POLYCARBONATE;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; DARIER DISEASE; EPIDERMIS; FILTER; HUMAN; HUMAN CELL; KERATINOCYTE; PRIMARY CULTURE; PRIORITY JOURNAL; PUNCH BIOPSY; SKIN BIOPSY;

EID: 84907338599     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/7651-2013-40     Document Type: Article

References (9)

1. Pruniéras M, Régnier M, Woodley D (1983) Methods for cultivation of keratinocytes with an air-liquid interface. J Invest Dermatol 81:28s-33s
2. Rosdy M, Clauss LC (1990) Terminal epidermal differentiation of human keratinocytes grown in chemically defined medium on inert filter substrates at the air-liquid interface. J Invest Dermatol 95:409-414
3. Coquette A, Berna N, Vandenbosch A, Rosdy M, Poumay Y (1999) Differential expression and release of cytokines by an in vitro reconstructed human epidermis following exposure to skin irritant and sensitizing chemicals. Toxicol In Vitro 13:867-877
4. Poumay Y, Coquette A (2007) Modelling the human epidermis in vitro: Tools for basic and applied research.ArchDermatolRes 298:361-369
5. Poumay Y, Dupont F, Marcoux S, Leclercq-SmekensM, HérinM, Coquette A (2004) A simple reconstructed human epidermis: Preparation of the culture model and utilization in in vitro studies. Arch Dermatol Res 296:203-211
6. Frankart A, Coquette A, Schroeder K-R, Poumay Y (2012) Studies of cell signalling in a reconstructed human epidermis exposed to sensitizers: IL-8 synthesis and release depends on EGFR activation. Arch Dermatol Res 304:289-303
7. Frankart A, Malaisse J, De Vuyst E, Minner F, Lambert de Rouvroit C, Poumay Y (2012) Epidermal morphogenesis during progressive in vitro 3D reconstruction at the air-liquid interface. Exp Dermatol 21:871-875
8. Minner F, Herphelin F, Poumay Y (2010) Study of epidermal differentiation in human keratinocytes cultured in autocrine conditions. Methods Mol Biol 585:71-82
9. Lambert de Rouvroit C, Charlier C, Lederer D, De Glas V, De Vuyst E, Dargent J-L, Grammatico P, Binni F, Rousseau C, Hennecker J-L, Nikkels AF, Poumay Y (2013) In vitro reconstruction of epidermis from primary Darier's disease keratinocytes replicates the histopathological phenotype. J Dermatol Sci 71:138-140