A multiplexed microfluidic droplet platform for Matrix Metalloproteinase screening

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

Volumn 3, Issue , 2013, Pages 1595-1597

  Rane, Tushar D ^a   Zee, Helena C ^a   Wang, Tza Huei ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

Confocal fluorescence spectroscopy; Droplet microfluidics; Matrix Metalloproteinases; MMPs

Indexed keywords

DROPS; ENZYME INHIBITION; FLUORESCENCE SPECTROSCOPY; SUBSTRATES;

CONFOCAL FLUORESCENCE SPECTROSCOPY; CONTINUOUS FLOWS; DROPLET MICROFLUIDICS; INDIVIDUAL DROPLETS; MATRIX METALLOPROTEINASES; MICROFLUIDIC DROPLETS; MMPS;

MICROFLUIDICS;

EID: 84907313815     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (5)

1. Roy, R.; Yang, J.; Moses, M. A. Matrix Metalloproteinases as Novel Biomarkers and Potential Therapeutic Targets in Human Cancer. J. Clin. Oncol. 2009, 27, 5287-5297.
2. Miller, M. A.; Barkal, L.; Jeng, K.; Herrlich, A.; Moss, M.; Griffith, L. G.; Lauffenburger, DA. Proteolytic Activity Matrix Analysis (PrAMA) for Simultaneous Determination of Multiple Protease Activities, Integr. Biol. (Camb) 2011, 3, 422-438.
3. Brouzes, E.; Medkova, M.; Savenelli, N; Marran, D.; Twardowski, M.; Hutchison, J. B.; Rothberg, J. M.; Link, D. R.; Perrimon, N.; Samuels, M. L. Droplet Microfluidic Technology for Single-Cell High-Throughput Screening. Proc. Natl. Acad. Sci. U. S. A. 2009, 106, 14195-14200.
4. Chen, C. H.; Miller, M. A.; Sarkar, A.; Beste, M. T.; Isaacson, K. B.; Lauffenburger, DA.; Griffith, L. G.; Han, J. Multiplexed Protease Activity Assay for Low-Volume Clinical Samples using Droplet-Based Microfluidics and its Application to Endometriosis. J. Am. Chem. Soc. 2013, 135, 1645-1648.
5. Zee, H; Rane, T. D.; Wang, T. H. Microfluidic Platform for on-Dem and Generation of Spatially Indexed Combinatorial Droplets. Lab. Chip 2012, 12, 3055-3062.