Detection and differentiation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and restriction enzyme analysis

Medical Mycology

Volumn 31, Issue 1, 1993, Pages 65-75

  Hopfer, R L ^a   Walden, P ^a   Setterquist, S ^b   Highsmith, W E ^a  

^a UNIVERSITY OF NORTH CAROLINA HOSPITALS   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RESTRICTION ENDONUCLEASE;

ARTICLE; CANDIDA ALBICANS; CANDIDIASIS; CRYPTOCOCCUS; DIAGNOSTIC TEST; DIAGNOSTIC VALUE; DNA PROBE; HUMAN; MYCOSIS; POLYMERASE CHAIN REACTION; TRICHOSPORON;

BASE SEQUENCE; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA, FUNGAL; ELECTROPHORESIS, AGAR GEL; FUNGI; HUMAN; MOLECULAR SEQUENCE DATA; MYCOSES; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SENSITIVITY AND SPECIFICITY;

EID: 84907124085     PISSN: 13693786     EISSN: None     Source Type: Journal    
DOI: 10.1080/02681219380000071     Document Type: Article

References (20)

1. Detection of surgical pathogens by in vitro DNA amplification. Part I. Rapid identification of Candida albicans by in vitro amplification of a fungus-specific gene
2. Development of DNA probes for Candida albicans
3. Molecular pathology and diagnosis of infectious diseases
4. The use of a DNA probe for epidemiological studies of candidiasis in immunocompromised hosts
5. A 96-well epifluorescence assay for rapid assessment of compounds inhibitory to Candida spp
6. Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species
7. Electrophoretic karyotypes and chromosome numbers in Candida species
8. Molecular probe for identification of medically important Candida species and Torulopsis glabrata
9. Variation of electrophoretic karyotypes among clinical isolates of Candida albicans
10. Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction
11. Serodiagnosis of candidosis
12. Isolation of a species-specific DNA fragment for the rapid detection of Candida albicans by polymerase chain reaction (PCR)
13. Characterization of the sequence of colonization and nosocomial candidemia using DNA fingerprinting and a DNA probe
14. Genetics of Candida albicans
15. Application of DNA typing methods to epidemiology and taxonomy of Candida species
16. Application of DNA typing methods to Candida albicans epidemiology and correlations with phenotype
17. The polymerase chain reaction and infectious diseases: hopes and realities
18. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
19. The polymerase chain reaction: miracle or mirage? A critical review of its uses and limitations in diagnosis and research