Use of shrna for stable suppression of chemokine receptor expression and function in human cancer cell lines

Methods in Molecular Biology

Volumn 1172, Issue , 2014, Pages 209-218

  Salazar, Nicole ^a   Muñoz, Daniel ^a   Hoy, James ^a   Lokeshwar, Bal L ^a  

^a NONE

Author keywords

CXCR7; Lipofectamine 2000; Selection; ShRNA; Stable transfection

Indexed keywords

CHEMOKINE RECEPTOR CXCR7; LIPOFECTAMINE; MESSENGER RNA; PUROMYCIN; SHORT HAIRPIN RNA; ANTIBODY; CHEMOKINE RECEPTOR CXCR; CXCR7 PROTEIN, HUMAN; LIPID; SMALL INTERFERING RNA;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; GENE EXPRESSION; GENE FUNCTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IN VIVO STUDY; LNCAP CELL LINE; MALE; MCF 7 CELL LINE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RNA INTERFERENCE; STABLE EXPRESSION; TUMOR XENOGRAFT; ANIMAL; ANTAGONISTS AND INHIBITORS; CELL CLONE; CHEMISTRY; GENETICS; METABOLISM; RABBIT; TUMOR CELL LINE; WESTERN BLOTTING;

ANIMALS; ANTIBODIES; BLOTTING, WESTERN; CELL LINE, TUMOR; CLONE CELLS; GENE EXPRESSION; HUMANS; LIPIDS; PLASMIDS; PROMOTER REGIONS, GENETIC; RABBITS; RECEPTORS, CXCR; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 84907063716     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0928-5_19     Document Type: Article

References (11)

1. Fire A, Xu S, Montgomery MK et al (1998) Potent and specifi c genetic interference bydouble-stranded RNA in Caenorhabditis elegans. Nature 391:806–811
2. “The Nobel Prize in Physiology or Medicine2006”. Nobelprize.org. Nobel Media AB(2013) http://www.nobelprize.org/nobel_prizes/medicine/laureates/2006/
3. Lee RC, Ambros V (2001) An extensive classof small RNAs in Caenorhabditis elegans. Science 294:862–864
4. Lee Y, Ahn C, Han J et al (2003) The nuclearRNase III Drosha initiates microRNA processing. Nature 425:415–419
5. Hutvágner G, McLachlan J, Pasquinelli AE et al (2001) A cellular function for the RNAinterferenceenzyme Dicer in the maturation ofthe let-7 small temporal RNA. Science 293:834–838
6. Brennecke A, Stark RB, Russell SM et al (2005) Principles of microRNA-target recognition. PLoS Biol 3:e85
7. Origene Hu ShRNA plasmid panels applicationguide OriGene Technologies (Rockville, MD)
8. O’Keefe EP (2013) Nucleic acid delivery:lentiviral and retroviral vectors. Labome.com
9. Singh RK, Lokeshwar BL (2011) The IL-8-regulated chemokine receptor CXCR7 stimulatesEGFR signaling to promote prostatecancer growth. Cancer Res 71:3268–3277
10. Shamaladevi N, Lyn DA, Escudero DO et al (2009) CXC receptor-1 silencing inhibitsandrogen-independent prostate cancer. CancerRes 69:8265–8274
11. Singh RK, Lokeshwar BL (2009) Depletion ofintrinsic expression of Interleukin-8 in prostatecancer cells causes cell cycle arrest, spontaneousapoptosis and increases the effi cacy of chemotherapeuticdrugs. Mol Cancer 8:57.doi: 10.1186/1476-4598-8-57