Modeling the lung: Design and development of tissue engineered macro- and micro-physiologic lung models for research use

Experimental Biology and Medicine

Volumn 239, Issue 9, 2014, Pages 1135-1169

  Nichols, Joan E ^a   Niles, Jean A ^a   Vega, Stephanie P ^a   Argueta, Lissenya B ^a   Eastaway, Adriene ^a   Cortiella, Joaquin ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

3D lung models; Engineered lung models; engineered organ models; micro physiologic lung; physiologic lung as a model; upper respiratory tract models

Indexed keywords

ARTICLE; CELL DIFFERENTIATION; CELLULAR STRESS RESPONSE; CLINICAL ASSESSMENT; CLINICAL RESEARCH; DISEASE MODEL; EXPERIMENTAL DESIGN; HUMAN; IN VITRO STUDY; LUNG DEVELOPMENT; LUNG DISEASE; LUNG FUNCTION; LUNG MODEL; NONHUMAN; PROCESS OPTIMIZATION; RESPIRATORY TRACT DISEASE; STEM CELL RESEARCH; TISSUE CULTURE TECHNIQUE; TISSUE ENGINEERING; TISSUE SCAFFOLD; TOXICOLOGY; VALIDATION STUDY; 3D LUNG MODELS; ANIMAL; BIOLOGICAL MODEL; CYTOLOGY; ENGINEERED LUNG MODELS; ENGINEERED ORGAN MODELS; EQUIPMENT; LUNG; METABOLISM; METHODOLOGY; MICRO-PHYSIOLOGIC LUNG; PHYSIOLOGIC LUNG AS A MODEL; REVIEW; UPPER RESPIRATORY TRACT MODELS;

3D LUNG MODELS; ENGINEERED LUNG MODELS; ENGINEERED ORGAN MODELS; MICRO-PHYSIOLOGIC LUNG; PHYSIOLOGIC LUNG AS A MODEL; UPPER RESPIRATORY TRACT MODELS;

ANIMALS; HUMANS; LUNG; MODELS, BIOLOGICAL; TISSUE CULTURE TECHNIQUES; TISSUE ENGINEERING;

EID: 84907016366     PISSN: 15353702     EISSN: 15353699     Source Type: Journal    
DOI: 10.1177/1535370214536679     Document Type: Article

References (208)

1. Hakkinen KM,Harunaga JS,Doyle AD,Yamada KM.Direct comparisons of the morphology, migration, cell adhesions, and actin cytoskeleton of fibroblasts in four different three-dimensional extracellular matrices.Tissue Eng Part A. 2011;17:713-24
2. Eke I,Cordes N.Radiobiology goes 3D: how ECM and cell morphology impact on cell survival after irradiation.Radiother Oncol. 2011;99:271-8
3. Cortiella J,Niles J,Cantu A,Brettler A,Pham A,Vargas G,Winston S,Wang J,Walls S,Nichols JE.Influence of acellular natural lung matrix on murine embryonic stem cell differentiation and tissue formation.Tissue Eng Part A. 2010;16:2565-80
4. Wikswo JP,Curtis EL,Eagleton ZE,Evans BC,Kole A,Hofmeister LH,Matloff WJ.Scaling and systems biology for integrating multiple organs on a chip.Lab Chip. 2013;13:3496-511
5. Huh D,Leslie DC,Matthews BD,Fraser JP,Jurek S,Hamilton GA,Thorneloe KS,McAlexander MA,Ingber DE.A human disease model of drug toxicity-induced pulmonary edema in a lung-on-a-chip microdevice.Sci Transl Med. 2012;4:1-8
6. Lechner JF,Haugen A,Autrup H,McClendon IA,Trump BF,Harris CC.Clonal growth of epithelial cells from normal adult human bronchus.Cancer Res. 1981;41:2294-304
7. Hoang ATN,Chen P,Juarez J,Sachamitr P,Billing B,Bosnjak L,Dahlén B,Coles M,Svensson M.Dendritic cell functional properties in a three-dimensional tissue model of human lung mucosa.Am J Physiol Lung C. 2012;302:L226-37
8. Ramirez RD,Sheridan S,Girard L,Sato M,Kim Y,Pollack J,Peyton M,Zou Y,Kurie JM,Dimaio JM,Milchgrub S,Smith AL,Souza RF,Gilbey L,Zhang X,Gandia K,Vaughan MB,Wright WE,Gazdar AF,Shay JW,Minna JD.Immortalization of human bronchial epithelial cells in the absence of viral oncoproteins.Cancer Res. 2004;64:9027-34
9. Twort C,Van Breemen C.Human airway smooth muscle in culture.Tissue Cell. 1988;20:339-44
10. Choe MM,Sporn PH,Swartz MA.Extracellular matrix remodeling by dynamic strain in a three-dimensional tissue-engineered human airway wall model.Am J Res Cell Mol. 2006;35:306-306
11. Ebina M,Takahashi T,Chiba T,Motomiya M.Cellular hypertrophy and hyperplasia of airway smooth muscles underlying bronchial asthma: a 3-D morphometric study.Am Rev Respir Dis. 1993;148:720-6
12. Knox AJ,Corbett L,Stocks J,Holland E,Zhu YM,Pang L.Human airway smooth muscle cells secrete vascular endothelial growth factor: up-regulation by bradykinin via a protein kinase C and prostanoid-dependent mechanism.FASEB J. 2001;15:2480-88
13. Cozens AL,Yezzi MJ,Kunzelmann K,Ohrui T,Chin L,Eng K,Finkbeiner WE,Widdicombe JH,Gruenert DC.CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells.Am J Res Cell Mol. 1994;10:38-47
14. Masui T,Wakefield LM,Lechner JF,LaVeck MA,Sporn MB,Harris CC.Type beta transforming growth factor is the primary differentiation-inducing serum factor for normal human bronchial epithelial cells.Proc Natl Acad Sci. 1986;83:2438-42
15. Forbes B,Shah A,Martin GP,Lansley AB.The human bronchial epithelial cell line 16HBE14o − as a model system of the airways for studying drug transport.Int J Pharm. 2003;257:161-17
16. Ehrhardt C,Kneuer C,Laue M,Schaefer UF,Kim KJ,Lehr CM.16HBE14o-human bronchial epithelial cell layers express P-glycoprotein, lung resistance-related protein, and caveolin-1.Pharm Res. 2003;20:545-51
17. Fogh J,Fogh JM,Orfeo T.One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.J Natl Cancer. 1977;59:221-6
18. Cavet ME,West M,Simmons NL.Transepithelial transport of the fluoroquinolone ciprofloxacin by human airway epithelial Calu-3 cells.Antimicrob Agents Chemother. 1997;41:2693-8
19. Florea BI,Cassara ML,Junginger HE,Borchard G.Drug transport and metabolism characteristics of the human airway epithelial cell line Calu-3.J Control Release. 2003;87:131-8
20. Foster KA,Avery ML,Yazdanian M,Audus KL.Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery.Int J Pharm. 2000;208:1-11
21. Shen BQ,Finkbeiner WE,Wine JJ,Mrsny RJ,Widdicombe JH.Calu-3: a human airway epithelial cell line that shows cAMP-dependent Cl-secretion.Am J Physiol Lung C. 1994;266:L493-50
22. Bell ET.Hyperplaisa of the pulmonary alveolar epithelium in disease.Am J Pathol. 1943;19:901-11
23. Fulcher ML, Gabriel S, Burns KA, Yankaskas JR, Randell SH. Well-differentiated human airway epithelial cell cultures. In: Mitry RR and Hughes RD (eds) Human cell culture protocols. Humana Press, 2005, pp.183–206.
24. Hackett TL,Warner SM,Stefanowicz D,Shaheen F,Pechkovsky DV,Murray LA,Argentieri R,Kicic A,Stick SM,Bai TR,Knight DA.Induction of epithelial–mesenchymal transition in primary airway epithelial cells from patients with asthma by transforming growth factor-β1.Am J Res Crit Care. 2009;180:122-33
25. Giard DJ,Aaronson SA,Todaro GJ,Arnstein P,Kersey JH,Dosik H,Parks WP.In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.J Natl Cancer. 1973;51:1417-23
26. Foster KA,Oster CG,Mayer MM,Avery ML,Audus KL.Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism.Exp Cell Res. 1998;243:359-66
27. Shapiro DL,Nardone LL,Rooney SA,Motoyama EK,Munoz JL.Phospholipid biosynthesis and secretion by a cell line (A549) which resembles type II alveolar epithelial cells.BBA-Lipid Lipid Met. 1978;530:197-207
28. Jaffe EA,Nachman RL,Becker CG,Minick CR.Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.J Clin Invest. 1973;52:2745-2745
29. Lidington EA,Moyes DL,McCormack AM,Rose ML.A comparison of primary endothelial cells and endothelial cell lines for studies of immune interactions.Transpl Immunol. 1999;7:239-46
30. Unger RE,Krump-Konvalinkova V,Peters K,Kirkpatrick CJ.In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1. 6R.Microvasc Res. 2002;64:384-97
31. Muanza K,Gay F,Behr C,Scherf A.Primary culture of human lung microvessel endothelial cells: a useful in vitro model for studying Plasmodium falciparum-infected erythrocyte cytoadherence.Res Immunol. 1996;147:149-63
32. Hewett PW,Murray JC.Human microvessel endothelial cells: isolation, culture and characterization.In Vitro Cell Dev Biol-Anim. 1993;29:823-30
33. Comhair SA,Xu W,Mavrakis L,Aldred MA,Asosingh K,Erzurum SC.Human primary lung endothelial cells in culture.Am J Res Cell Mol. 2012;46:723-723
34. Hermanns MI,Fuchs S,Bock M,Wenzel K,Mayer E,Kehe K,Bittinger F,Kirkpatrick CJ.Primary human coculture model of alveolo-capillary unit to study mechanisms of injury to peripheral lung.Cell Tissue Res. 2009;336:91-105
35. Jacobs JP,Jones CM,Baille JP.Characteristics of a human diploid cell designated MRC-5.Nature. 1970;227:168-70
36. Boeckh M,Gleaves CA,Bindra R,Meyers JD.Comparison of MRC-5 and U-373MG astrocytoma cells for detection of cytomegalovirus in shell vial centrifugation cultures.Eur J of Clin Microbiol. 1991;10:569-72
37. Hayflick L.The limited in vitro lifetime of human diploid cell strains.Exp Cell Res. 1965;37:614-36
38. Kalimi M,Seifter S.Glucocorticoid receptors in WI-38 fibroblasts Characterization and changes with population doubling in culture.BBA-Gen Subj. 1979;583:352-59
39. Allen RG,Tresini M,Keogh BP,Doggett DL,Cristofalo VJ.Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38).J Cell Physiol. 1999;180:114-22
40. He Z,Huang S,Li Y,Zhang Q.Human embryonic stem cell lines preliminarily established in China.Zhonghua Yi Xue Za Zhi. 2002;82:1314-1314
41. Takahashi K,Tanabe K,Ohnuki M,Narita M,Ichisaka T,Tomoda K,Yamanaka S.Induction of pluripotent stem cells from adult human fibroblasts by defined factors.Cell. 2007;131:861-72
42. Cox JL,Rizzino A.Induced pluripotent stem cells: what lies beyond the paradigm shift.Exp Biol Med. 2010;235:148-58
43. Gunaseeli I,Doss MX,Antzelevitch C,Hescheler J,Sachinidis A.Induced pluripotent stem cells as a model for accelerated patient- and disease-specific drug discovery.Curr Med Chem. 2010;17:759-66
44. O’Malley J.New strategies to generate induced pluripotent stem cells.Curr Opin Biotech. 2009;20:516-21
45. Nichols JE,Niles J,Riddle M,Vargas G,Schilagard T,Ma L,Edward K,La Francesca S,Sakamoto J,Vega S,Ogedegbe M,Mlcak R,Devo D,Woodson L,McQuitty C,Lick S,Beckles D,Melo E,Cortilla J.Production and assessment of decellularized pig and human lung scaffolds.Tissue Eng Part A. 2013;19:2045-62
46. Pugin J,Dunn I,Jolliet P,Tassaux D,Magnenat JL,Nicod LP,Chevrolet JC.Activation of human macrophages by mechanical ventilation in vitro.Am J Physiol. 1998;275:L1040-50
47. Hermanns MI,Unger RE,Kehe K,Peters K,Kirkpatrick CJ.Lung epithelial cell lines in coculture with human pulmonary microvascular endothelial cells: development of an alveolar-capillary barrier in vitro.Lab Invest. 2004;84:736-52
48. Huh D,Matthews BD,Mammoto A,Montoya-Zavala M,Hsin HY,Ingber DE.Reconstituting organ-level lung functions on a chip.Science. 2010;328:1662-8
49. Ott HC,Clippinger B,Conrad C,Schuetz C,Pomerantseva I,Ikonomou L,Kotton D,Vacanti JP.Regeneration and orthotopic transplantation of a bioartificial lung.Nat Med. 2010;16:927-333
50. Kloxin AM,Lewis KJ,DeForest CA,Seedorf G,Tibbitt MW,Balasubramaniam V,Anseth KS.Responsive culture platform to examine the influence of microenvironmental geometry on cell function in 3D.Integr Biol (Camb). 2012;4:1540-9
51. Patel H,Kwon S.Multi-walled carbon nanotube-induced inflammatory response and oxidative stress in a dynamic cell growth environment.J Biol Eng. 2012;6:22-22
52. Horie M,Saito A,Mikami Y,Ohshima M,Morishita Y,Nakajima J,Kohyama T,Nagase T.Characterization of human lung cancer-associated fibroblasts in three-dimensional in vitro co-culture model.Biochem Biophys Res Commun. 2012;423:158-63
53. Mishra DK, Creighton CJ, Zhang Y, Gibbons DL, Kurie JM, Kim MP. Gene expression profile of A549 cells from tissue of 4D model predicts poor prognosis in lung cancer patients. Int J Cancer 2013. Epub ahead of print 2013. DOI: 10.1002/ijc.28428.
54. Blau H,Guzowski DE,Siddiqi ZA,Scarpelli EM,Bienkowski RS.Fetal type 2 pneumocytes form alveolar-like structures and maintain long-term differentiation on extracellular matrix.J Cell Physiol. 1998;136:203-14
55. Chinoy MR,Antonio-Santiago MT,Scarpelli EM.Maturation of undifferentiated lung epithelial cells into type II cells in vitro: a temporal process that parallels cell differentiation in vivo.Anat Rec. 1994;240:545-54
56. Chen P,Marsilio E,Goldstein RH,Yannas IV,Spector M.Formation of lung alveolar-like structures in collagen-glycosaminoglycan scaffolds in vitro.Tissue Eng. 2005;11:1436-48
57. Mondrinos MJ,Koutzaki S,Jiwanmall E,Li M,Dechadarevian JP,Lelkes PI,Fink CM.Engineering three-dimensional pulmonary tissue constructs.Tissue Eng. 2006;12:717-28
58. Mondrinos MJ,Koutzaki S,Lelkes PI,Finck CM.A tissue-engineered model of fetal distal lung tissue.Am J Physiol Lung Cell Mol Physiol. 2007;293:L639-50
59. Andrade CF,Wong AP,Waddell TK,Keshavjee S,Liu M.Cell-based tissue engineering for lung regeneration.Am J Physiol. 2007;292:L510-8
60. Price AP,England KA,Matson AM,Blazar BR,Panoskaltsis-Mortari A.Development of a decellularized lung bioreactor system for bioengineering the lung: the matrix reloaded.Tissue Eng Part A. 2010;16:2581-91
61. Coraux C,Nawrocki-Raby B,Hinnrasky J,Kileztky C,Gaillard D,Dani C,Puchelle E.Embryonic stem cells generate airway epithelial tissue.Am J Respir Cell Mol Biol. 2005;32:87-92
62. Cortiella J,Nichols JE,Kojima K,Bonassar LJ,Dargon P,Roy AK,Vacant MP,Niles JA,Vacanti CA.Tissue-engineered lung: an in vivo and in vitro comparison of polyglycolic acid and pluronic F-127 hydrogel/somatic lung progenitor cell constructs to support tissue growth.Tissue Eng. 2006;12:1213-25
63. Zani BG,Kojima K,Vacanti CA,Edelman ER.Tissue-engineered endothelial and epithelial implants differentially and synergistically regulate airway repair.Proc Natl Acad Sci U S A. 2008;105:7046-51
64. Nichols JE,LeDuc JVaccines. Barrett ADTStanberry LR, ed. London, UK: Academic Press; 2009:498-515.
65. Lin YM,Zhang A,Rippon HJ,Bismarck A,Bishop AE.Tissue engineering of lung: the effect of extracellular matrix on the differentiation of embryonic stem cells to pneumocytes.Tissue Eng Part A. 2010;16:1515-26
66. Huang SX,Islam MN,O’Neill J,Hu Z,Yang YG,Chen YW,Mumau M,Green MD,Vunjak-Novakovic G,Bhattacharya J,Snoeck HW.Efficient generation of lung and airway epithelial cells from human pluripotent stem cells.Nat Biotechnol. 2014;32:84-91
67. Ghaedi M,Calle EA,Mendez JJ,Gard AL,Balestrini J,Booth A,Bove PF,Gui L,White ES,Niklason LE.Human iPS cell-derived alveolar epithelium repopulates lung extracellular matrix.J Clin Invest. 2013;123:4950-62
68. Mou H,Zhao R,Sherwood R,Ahfeldt T,Lapey A,Wain J,Sicilian L,Izvolsky K,Musunuru K,Cowan C,Rajagopal J.Generation of multipotent lung and airway progenitors from mouse ESCs and patient-specific cystic fibrosis iPSCs.Cell Stem Cell. 2012;10:385-97
69. Nichols JE,Cortiella J.Engineering of a complex organ: progress toward development of a tissue-engineered lung.Proc Am Thorac Soc. 2008;5:723-30
70. Nichols JE,Niles JA,Cortiella J.Design and development of tissue engineered lung.Organogenesis. 2009;5:57-61
71. Nichols JE,Niles JA,Cortiella J.Production and utilization of acellular lung scaffolds in tissue engineering.J Cell Biochem. 2012;113:2185-92
72. Choe MM,Tomei AA,Swartz MA.Physiological 3D tissue model of the airway wall and mucosa.Nat Protoc. 2006;1:357-62
73. Miller C,George S,Niklason L.Developing a tissue-engineered model of the human bronchiole.J Tissue Eng Regen Med. 2010;4:619-27
74. Singh D,Zo SM,Kumar A,Han SS.Engineering three-dimensional macroporous hydroxyethyl methacrylate-alginate-gelatin cryogel for growth and proliferation of lung epithelial cells.J Biomater Sci Polym Ed. 2013;24:1343-59
75. Petersen TH,Calle EA,Zhao L,Lee EJ,Gui L,Raredon MB,Gavrilov K,Yi T,Zhuang ZW,Breuer C,Herzog E,Niklason LE.Tissue-engineered lungs for in vivo implantation.Science. 2010;329:538-41
76. Crapo PM,Gilbert TW,Badylak SF.An overview of tissue and whole organ decellularization processes.Biomaterials. 2011;32:3233-43
77. Lwebuga-Mukasa JS,Ingbar DH,Madri JA.Repopulation of a human alveolar matrix by adult rat type II pneumocytes in vitro: a novel system for type II pneumocyte culture.Exp Cell Res. 1986;162:423-35
78. Liu Y, Singh AK. Microfluidic platforms for single-cell protein analysis. J Lab Autom 2013. Epub ahead of print 2013. DOI: 10.1177/2211068213494389.
79. Delamarche E,Tonna N,Lovchik RD,Bianco F,Matteoli M.Pharmacology on microfluidics: multimodal analysis for studying cell-cell interaction.Curr Opin Pharmacol. 2013;13:821-8
80. Ruiz SA,Chen CS.Microcontact printing: a tool to pattern.Soft Matter. 2007;3:168-77
81. Sin ML,Gao J,Liao JC,Wong PK.System integration – a major step toward lab on a chip.J Biol Eng. 2011;5:6-6
82. Ren K,Chen Y,Wu H.New materials for microfluidics in biology.Anal Biotech. 2014;25:78-85
83. Arshad A. Drug-induced pulmonary toxicity workup. Medscape WebMD LLC 20 Nov 2013. http://emedicine.medscape.com/article/1343451-workup.
84. Bonniaud Ph, Baudouin N, Fanton A, Camus C, Favrolt N, Guerriaud M, Jacquet L. The drug-induced respiratory disease website. Pneumotox On Line, http://www.pneumotox.com/.
85. Sugihara H,Toda S,Miyabara S,Fujiyama C,Yonemitsu N.Reconstruction of alveolus-like structure from alveolar type II epithelial cells in a three-dimensional collagen gel matrix culture.Am J Pathol. 1993;142:783-92
86. Chakir J,Pagé N,Hamid Q,Laviolette M,Boulet LP,Rouabhia M.Bronchial mucosa produced by tissue engineering: a new tool to study cellular interactions in asthma.J Allergy Clin Immunol. 2001;107:36-40
87. Paquette JS,Moulin V,Tremblay P,Bernier V,Boutet M,Laviolette M,Auger FA,Boulet LP,Goulet F.Tissue-engineered human asthmatic bronchial equivalents.Eur Cell Mater. 2004;7:1-11
88. Matrosovich MN,Matrosovich TY,Gray T,Roberts NA,Klenk HD.Human and avian influenza viruses target different cell types in cultures of human airway epithelium.Proc Natl Acad Sci U S A. 2004;101:4620-4
89. Birkness KA,Guarner J,Sable SB,Tripp RA,Kellar KL,Bartlett J,Quinn FD.An in vitro model of the luekocyte interactions associated with granuloma formation in Mycobacterium tuberculosis infection.Immunol Cell Biol. 2007;85:160-8
90. Nguyen Hoang AT,Chen P,Juarez J,Sachamitr P,Billing B,Bosnjak L,Dahlén B,Coles M,Svensson M.Dendritic cell functional properties in a three-dimensional tissue model of human lung mucosa.Am J Physiol Lung Cell Mol Physiol. 2012;302:L226-37
91. Booth AJ,Hadley R,Cornett AM,Dreffs AA,Matthes SA,Tsui JL,Weiss K,Horowitz JC,Fiore VF,Barker TH,Moore BB,Martinez FJ,Nicklason LE,White ES.Aceullar normal and fibrotic human lung matrices as a culture system for in vitro investigation.Am J Respir Crit Care Med. 2012;186:866-76
92. Bonvillain RW,Danchuk S,Sullivan DE,Betancourt AM,Semon JA,Eagle ME,Mayeux JP,Gregory AN,Wang G,Townley IK,Borg ZD,Weiss DJ,Bunnell BA.A nonhuman primate model of lung regeneration: detergent-mediated decellularization and initial in vitro recellularization with mesenchymal stem cells.Tissue Eng Part A. 2012;18:2437-52
93. Qasaimeh MA,Ricoult SG,Juncker D.Microfluidic probes for use in life sciences and medicine.Lab Chip. 2013;13:40-50
94. Han C,Pang S,Bower DV,Yiu P,Yang C.Wide field-of-view on-chip Talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator.Anal Chem. 2013;85:2356-60
95. Whitesides GM.The origins and the future of microfluidics.Nature. 2006;442:368-73
96. White AK,VanInsberghe M,Petriv OI,Hamidi M,Sikorski D,Marra MA,Piret J,Aparicio S,Hansen CL.High-throughput microfluidic single-cell RT-qPCR.Proc Natl Acad Sci U S A. 2011;108:13999-4004
97. Zhang Z,Nagrath S.Microfluidics and cancer: are we there yet?.Biomed Microdevices. 2013;15:595-609
98. Tang T,Badal MY,Ocvirk G,Lee WE,Bader DE,Bekkaoui F,Harrison DJ.Integrated microfluidic electrophoresis system for analysis of genetic materials using signal amplification methods.Anal Chem. 2002;74:725-33
99. Hong JW,Quake SR.Integrated nanoliter systems.Nat Biotechnol. 2003;21:1179-83
100. Winer-Jones JP,Vahidi B,Arquilevich N,Fang C,Ferguson S,Harkins D,Hill C,Klem E,Pagano PC,Peasley C,Romero J,Shartle R,Vasko RC,Strauss WM,Dempsey PW.Circulating tumor cells: clinically relevant molecular access based on a novel CTC flow Cell.PLoS One. 2014;9:e86717-e86717
101. Sequist LV,Nagrath S,Toner M,Haber DA,Lynch TJ.The CTC-chip: an exciting new tool to detect circulating tumor cells in lung cancer patients.J Thorac Oncol. 2009;4:281-3
102. Fluidigm Corporation.http://www.mscience.com.au/upload/pages/singlecell/c1_mrna_ brochure-br100-6199-a1.pdf?1391142668.
103. Chan MC,Chan RW,Yu WC,Ho CC,Yuen KM,Fong JH,Tang LL,Lai WW,Lo AC,Chui WH,Sihoe AD,Kwong DL,Wong DS,Tsao GS,Poon LL,Guan Y,Nicholls JM,Peiris JS.Tropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract.Am J Pathol. 2010;176:1828-40
104. Zhang L,Bukreyev A,Thompson CI,Watson B,Peeples ME,Collins PL,Pickles RJ.Infection of ciliated cells by human parainfluenza virus type 3 in an in vitro model of human airway epithelium.J Virol. 2005;79:1113-24
105. Chan MC,Chan RW,Yu WC,Ho CC,Chui WH,Lo CK,Yuen KM,Guan YI,Nicholls JM,Peiris JS.Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells.Respir Res. 2009;10:102-102
106. Diebel LN,Liberati DM,Diglio CA,Brown WJ.Immunoglobulin A modulates inflammatory responses in an in vitro model of pneumonia.J Trauma. 2005;59:1099-106
107. Nalayanda DD,Wang Q,Fulton WB,Wang TH,Abdullah F.Engineering an artificial alveolar-capillary membrane: a novel continuously perfused model within microchannels.J Pediatr Surg. 2010;45:45-51
108. Nalayanda DD,Fulton WB,Wang TH,Abdullah F.A multiphase fluidic platform for studying ventilator-induced injury of the pulmonary epithelial barrier.Integr Biol (Camb). 2013;5:1141-8
109. Zamir M,Moore JE,Fujioka H,Gaver DP.Biofluid mechanics of special organs and the issue of system control.Ann Biomed Eng. 2010;38:1204-15
110. Evangelatov A, Pankov R. The evolution of three-dimensional cell cultures towards unimpeded regenerative medicine. Regenerative Medicine and Tissue Engineering. Prof. Jose A Andrades (Ed.) web publication by INTECH OPEN, http://www.intechopen.com/books/regenerative-medicine-and-tissue-engineering/the-evolution-of-three-dimensional-cell-cultures-towards-unimpeded-regenerative-medicine-and-tissue- (2013, accessed on 12 May 2014), ISBN: 978-953-51-1108-5.
111. Ingber DE.Tensegrity and mechanotransduction.J Bodyw Mov Ther. 2008;12:198-200
112. Lin YM,Zhang A,Rippon HJ,Bismarck A,Bishop AE.Tissue engineering of lung: the effect of extracellular matrix on the differentiation of embryonic stem cells to pneumocytes.Tissue Eng Part A. 2010;16:1515-26
113. Luque T,Melo E,Garreta E,Cortiella J,Nichols J,Farré R,Navajas D.Local micromechanical properties of decellularized lung scaffolds measured with atomic force microscopy.Acta Biomater. 2013;9:6852-9
114. Shamis Y,Hasson E,Soroker A,Bassat E,Shimoni Y,Ziv T,Sionov RV,Mitrani E.Organ-specific scaffolds for in vitro expansion, differentiation, and organization of primary lung cells.Tissue Eng Part C Methods. 2011;17:861-70
115. Luque T,Melo E,Garreta E,Cortiella J,Nichols J,Farre R,Navajas D.Local micromechanical properties of decellularized lung scaffolds measured with atomic force microscopy.Acta Biomater. 2013;9:6852-9
116. Huh D,Hamilton GA,Ingber DE.From 3D cell culture to organs-on-chips.Trends Cell Biol. 2011;21:745-54
117. Folch A,Toner M.Microengineering of cellular interactions.Annu Rev Biomed Eng. 2000;2:227-56
118. Khademhosseini A,Langer R,Borenstein J,Vacanti JP.Microscale technologies for tissue engineering and biology.Proc Natl Acad Sci U S A. 2006;103:2480-87
119. Liu M,Transwell AK,Post M.Mechanical force-induced signal transduction in lung cells.Am J Physiol. 1999;277:L667-83
120. Schwartz MA.Integrin signaling revisited.Trends Cell Biol. 2001;11:466-70
121. Vogel V,Sheetz MP.Cell fate regulation by coupling mechanical cycles to biochemical signaling pathways.Curr Opin Cell Biol. 2009;21:38-46
122. Lukashev ME,Werb Z.ECM signalling: orchestrating cell behaviour and misbehaviour.Trends Cell Biol. 1998;8:437-41
123. Han B,Lodyga M,Liu M.Ventilator-induced lung injury: role of protein-protein interaction in mechanosensation.Proc Am Thorac Soc. 2005;2:181-7
124. Wang Y,Maciejewski BS,Weissmann G,Silbert O,Han H,Sanchez-Esteban J.DNA microarray reveals novel genes induced by mechanical forces in fetal lung type II epithelial cells.Pediatr Res. 2006;60:118-24
125. Wang Y,Huang Z,Nayak PS,Matthews BD,Warburton D,Shi W,Sanchez-Esteban J.Strain-induced differentiation of fetal type II epithelial cells is mediated via the integrin α6β1-ADAM17/tumor necrosis factor-α-converting enzyme (TACE) signaling pathway.J Biol Chem. 2013;288:25646-57
126. Gossett DR,Tse HTK,Lee SA,Ying Y,Lindgren AG,Yang OO,Rao J,Clark AT,Carlo DD.Hydrodynamic stretching of single cells for large population mechanical phenotyping.Proc Natl Acad Sci U S A. 2012;109:7630-35
127. Polacheck WJ,Li R,Uzel SGM,Kamm RD.Microfluidic platforms for mechanobiology.Lab Chip. 2013;13:2252-67
128. Kim DH,Wong PK,Park J,Levchenko A,Sun Y.Microengineered platforms for cell mechanobiology.Annu Rev Biomed Eng. 2009;11:203-33
129. Goldblum SE,Yoneda K,Cibull M,Pearson T,Hall C,Marshall ME.Human recombinant interleukin-2 provokes acture pulmonary vascular endothelial injury in rabbits.J Biol Response Mod. 1990;9:127-39
130. Matute-Bello G,Frevert CW,Martin TR.Animal models of acute lung injury.Am J Physiol Lung Cell Mol Physiol. 2008;295:L379-99
131. Chen Y,Guo H,Xu D,Xu X,WEang H,Hu X,Lu Z,Kwak D,Xu Y,Gunther R,Huo Y,Weir EK.Left ventricular failure produces profound lung remodeling and pulmonary hypertension in mice: heart failure causes severe lung disease.Hypertension. 2012;59:1170-78
132. Murakami K,Okajima K,Uchiba M,Johno M,Nakagaki T,Okabe H,Takatsuki K.Activated protein C attenuates endotoxin-induced pulmonary vascular injury by inhibiting activated leukocytes in rats.Blood. 1996;87:642-7
133. Berger MM,Rozendal CS,Schieber C,Dehler M,Zugel S,Bardenheuer HJ,Bartsch P,Mairbaurl H.The effect of endothelin-1 on alveolar fluid clearance and pulmonary edema formation in the rat.Anesth Analg. 2009;108:225-31
134. Gross P.Enzymatically produced pulmonary emphysema: a preliminary report.J Occup Med. 2006;6:481-481
135. Senior RM,Tegner H,Kuhn C,Ohlsson K,Strarcher BC,Pierce JAL.The induction of pulmonary emphysema with human leukocyte elastase.Am Rev Respir Dis. 1977;116:469-469
136. Atunes MA,Rocco PRM.Elastase-induced pulmonary emphysema: insights from experimental models.An Acad Bras Cienc. 2011;83:1385-95
137. Bánhegyi G,Braun L,Csala M,Puskás F,Mandl J.Ascorbate metabolism and its regulation in animals.Free Radic Biol Med. 1997;23:793-803
138. Huizar I,Malur A,Patel J,McPeek M,Dobbs L,Wingard C,Barna BP,Thomassen MJ.The role of PPARγ in carbon nanotube-elicited granulomatous lung inflammation.Respir Res. 2013;14:7-7
139. Costa CLB,Spilborghs GMFT,Martins MA,Saldiva PHN,Mauad T.Nitric acid-induced bronchiolitis in rats mimics childhood bronchiolitis obliterans.Respiration. 2005;72:642-9
140. Palmer SM,Flake GP,Kelly FL,Zhang HL,Nugent JL,Kirby PJ,Foley JF,Gwinn WM,Morgan DL.Severe airway epithelial injury, aberrant repair and bronchiolitis obliterans develops after diacetyl instillation in rats.PloS One. 2011;6:e17644-e17644
141. Tavana H,Huh D,Grotber JB,Takayama S.Microfluidics, lung surfactant, and respiratory disorders.Lab Med. 2009;40:203-9
142. Barker AF.Bronchiectasis.N Engl J Med. 2002;346:1383-93
143. Reid L.An experimental study of hypersecretion of mucus in the bronchial tree.Br J Exp Pathol. 1963;44:437-45
144. Rogers CS,Stoltz DA,Meyerholz DK,Ostedgaard LS,Rokhlina T,Taft PJ,Rogan MP,Pezzulo AA,Karp PH,Itani OA,Kabel AC,Wohlford-Lenane CL,Davis GJ,Hanfland RA,Smith TL,Samuel M,Wax D,Murphy CN,Rieke A,Whitworth K,Uc A,Starner TD,Brogden KA,Shilyansky J,McCray PB,Zabner J,Prather RS,Welsh MJ.Distruption of the CFTR gene produces a model of cystic fibrosis in newborn pigs.Science. 2008;321:1837-41
145. Stoltz DA,Rokhlina T,Ernst SE,Pezzulo AA,Ostedgaard LS,Karp PH,Samuel MS,Reznikov LR,Rector MV,Gansemer ND,Bouzek DC,Alaiwa MH,Hoegger MJ,Ludwig PS,Taft PJ,Wallen TJ,Wohlford-Lenane C,McMenimen JD,Chen JH,Bogan KL,Adam RJ,Hornick EE,Nelson GA,Hoffman EA,Chang EH,Zabner J,McCray PB,Prather RS,Meyerholz DK,Welsh MJ.Intestinal CFTR expression alleviates meconium ileus in cystic fibrosis pigs.J Clin Invest. 2013;123:2685-93
146. Clarke LL,Grubb BR,Gabriel SE,Smithies O,Koller BH,Boucher RC.Defective epithelial chloride transport in a gene-targeted mouse model of cystic fibrosis.Science. 1992;257:1125-8
147. Snouwaert JN,Brigman KK,Latour Am,Malouf NN,Boucher RC,Smithies O,Koller BH.An animal model for cystic fibrosis made by gene targeting.Science. 1992;257:1083-88
148. Meeks M,Bush A.Primary ciliary dyskinesia (PCD).Pediatr Pulmonol. 2000;29:307-16
149. Craig VJ,Quintero PA,Fyfe SE,Patel AS,Knolle MD,Kobzik L,Owen CA.Profibrotic activities for matrix metalloproteinase-8 during bleomycin-mediated lung injury.J Immunol. 2013;190:4283-96
150. Jiang D,Liang J,Hodge J,Lu B,Zhu Z,Yu S,Fan J,Gao Y,Yin Z,Homer R,Gerard C,Noble PW.Regulation of pulmonary fibrosis by chemokine receptor CXCR3.J Clin Invest. 2004;14:291-9
151. Tager AM,Kradin RL,LaCamera P,Bercury SD,Campanella GS,Leary CP,Polosukhin V,Zhao LH,Sakamoto H,Blackwell TS,Luster AD.Inhibition of pulmonary fibrosis by the chemokine IP-10/CXCL10.Am J Respir Cell Mol Biol. 2004;31:395-404
152. Baron RM,Choi AJS,Owen CA,Choi AM.Genetically manipulated mouse models of lung disease: potential and pitfalls.AJP-Lung Cell Mol Physiol. 2012;302:L485-L97
153. Haston CK,Wan M,Dejournett RE,Zhou X,Ni D,Gu X,King TM,Weil MM,Newman RA,Amos CI,Travis EL.Bleomycin hydrolase and a geneti locus within the MHC affect risk for pulmonary fibrosis in mice.Hum Mol Genet. 2002;11:1855-63
154. Chen Y,Guo H,Xu D,Xu X,Wang H,Hu X,Lu Z,Kwak D,Xu Y,Gunther R,Huo Y,Weir EK.Left ventricular failure produces profound lung remodeling and pulmonary hypertension in mice: heart failure causes severe lung disease.Hypertension. 2012;59:1170-78
155. Sisson TH,Mendez M,Choi K,Subbotina N,Courey A,Cunningham A,Dave A,Engelhardt JF,Liu X,White ES,Thannickal VJ,Moore BB,Christensen PJ,Simon RH.Targeted injury of type II alveolar epithelial cells induces pulmonary fibrosis.Am J Respir Crit Care Med. 2010;181:254-63
156. Osterholzer JJ,Olsewski MA,Murdock BJ,Chen GH,Erb-Downward JR,Subbotina N,Browning K,Lin Y,Morey RE,Dayrit JK,Horowitz JC,Simon RH,Sisson TH.Implicating exudate macrophages and Ly-Chigh monocytes in CCR2-dependent lung fibrosis following gene-targeted alveolar injury.Am J Respir Crit Care Med. 2011;183:A6148-A6148
157. Sayes CM,Reed KL,Warheit DB.Assessing toxicity of fine and nanoparticles: comparing in vitro measurements to in vitro pulmonary toxicity profiles.Toxicol Sci. 2007;97:163-80
158. Zhu Z,Yang G,Wang Y,Yang J,Ai G,Niu P,Tian L.Suppression of thioredoxin system contributes to silica-induced oxidative stress and pulmonary fibrogenesis in rats.Toxicol Lett. 2013;222:289-94
159. Mossman BT,Lippmann M,Hesterberg TW,Kelsey KT,Barchowsky A,Bonner JC.Pulmonary endpoints (lung carcinomas and asbestosis) following inhalation exposure to asbestos.J Toxicol Environ Health B Crit Rev. 2011;14:76-121
160. Liu G,Cheresh P,Kamp DW.Molecular basis of asbestos-induced lung disease.Ann Rev Pathol Mech Dis. 2013;8:161-87
161. Brody AR,Hill LH,Adkins B,O’Connor RW.Chrysotile asbestoas inhalation in rats: deposition pattern and reaction of alveolar epithelium and pulmonary macrophages.Am Rev Respir Dis. 1981;123:670-79
162. Vernath JM,Cutler NS,Kaser EG,Reilly CA,Yost GS.Effects of cell type and culture media on Interleukin-6 secretion in response to environmental particles.Toxicol In Vitro. 2008;22:498-509
163. Yao S,Chen HH,Harte E,Ventura GD,Petibois C.The role of asbestos morphology on their cellular toxicity: an in vitro 3D Raman/Rayleigh imaging study.Anal Bioanal Chem. 2013;405:8701-7
164. Sussman RG,Cohen BS,Lippmann M.Asbestoas fiber deposition in a human tracheobronchial cast I.Exp Inhal Toxicol. 1992;3:145-60
165. Das M,Fessel J,Tang H,West J.A process-based review of mouse models of pulmonary hypertension.Pulm Circ. 2012;2:415-33
166. Gomez-Arroyo JG,Farkas L,Alhussaini AA,Farkas D,Kraskauskas D,Voelkel NF,Bogaard HJ.The monocrotaline model of pulmonary hypertension in perspective.Am J Physiol Lung Cell Mol Physiol. 2012;302:L363-9
167. van Suylen Rj,Smits JF,Daemen MJ.Pulmonary artery remodeling differs in hypoxia- and monocrotaline-induced pulmonary hypertension.Am J Respir Crit Care Med. 1988;157:1423-8
168. Chen Y,Guo H,Xu D,Xu X,Wang H,Hu X,Lu Z,Kwak D,Xu Y,Gunther R,Huo Y,Weir EK.Left ventricular failure produces profound lung remodeling and pulmonary hypertension in mice: heart failure causes severe lung disease.Hypertension. 2012;59:1170-78
169. Ciuclan L,Bonneau O,Hussey M,Duggan N,Holmes AM,Good R,Stringer R,Jones P,Morrell NW,Jarai G,Walker C,Westwick J,Thomas M.A novel murine model of severe pulmonary arterial hypertension.Am J Respir Crit Care Med. 2011;184:1174-82
170. Abe K,Toba M,Alzoubi A,Ito M,Fagan KA,Cool CD,Voelkel NF,McMurtry IF,Oka M.Formation of plexiform lesions in experimental severe pulmonary arterial hypertension.Circulation. 2010;121:2747-54
171. Munster VJ,de Wit E,van den Brand JM,Herfst S,Schrauwen EJA,Bestebroer TM,van de Vijver D,Boucher CA,Koopmans M,Rimmelzwaan GF,Kuiken T,Osterhaus ADME,Fouchier RAM.Pathogenesis and transmission of swine-origin 2009 A(H1N1) influenza virus in ferrets.Science. 2009;325:481-3
172. Kraus TA,Garza L,Horvath CM.Enabled interferon signaling evasion in an immune-competent transgenic mouse model of parainfluenza virus 5 infection.Virology. 2008;371:196-205
173. Jafri HS,Chavez-Bueno S,Mejias A,Gomez AM,Rios AM,Nassi SS,Yusuf M,Kapur P,Hardy RD,Hatfield J,Rogers BB,Krisher K,Ramilo O.Respiratory syncytial virus induces pneumonia, cytokine response, airway obstruction, and chronic inflammatory infiltrates associated with long-term airway hyperresponsiveness in mice.J Infect Dis. 2004;189:1856-65
174. Rimmelzwaan GF,Kuiken T,van Amerongen G,Bestebroer TM,Fouchier RA,Osterhaus AD.Pathogenesis of influenza A (H5N1) virus infection in a primate model.J Virol. 2001;75:6687-6687
175. Calderon VE,Valbuena G,Goez Y,Judy BM,Huante MB,Sutjita P,Johnston RK,Estes DM,Hunter RL,Actor JK,Cirillo JD,Endsley JJ.A humanized mouse model of tuberculosis.PLoS One. 2013;8:e63331-e63331
176. Villenave R,Thavagnanam S,Sarlang S,Parker J,Douglas I,Skibinski G,Heaney LG,McKaigue JP,Coyle PV,Shields MD,Power UF.In vitro modeling of respiratory syncytial virus infection of pediatric bronchial epithelium, the primary target of infection in vivo.Proc Natl Acad Sci U S A. 2012;109:5040-5
177. van den Brand JM,Stittelaar KJ,Leijten LM,van Amerongen G,Simon JH,Osterhaus AD,Kuiken T.Modification of the ferret model for pneumonia from seasonal human influenza A virus infection.Vet Pathol. 2012;49:562-562
178. Tateda K,Takashima K,Miyazaki H,Matsumoto T,Hatori T,Yamaguchi K.Noncompromised penicillin-resistant pneumococal pneumonia CBA/J mouse model and comparative efficiencies of antibiotics in this model.Antimicrob Agents Chemother. 1996;40:1520-5
179. Xu L,Bao L,Dend W,Zhu H,Chen T,Lv Q,Li F,Yuan J,Xiang Z,Gao K,Xu Y,Huang L,Li Y,Liu J,Yao Y,Yu P,Yong W,Wei Q,Zhang L,Qin C.The mouse and ferret models for studying the novel avian-origin human influenza A (H7N9) virus.Virol J. 2013;10:253-253
180. Saladino RA,Stack AM,Fleisher GR,Thompson CM,Briles DE,Kobzik L,Siber GR.Development of a model of low-inoculum streptococcus pneumonia intrapulmonary infection in infant rats.Infect Immun. 1997;65:4701-4
181. Reed DS,Smith L,Dunsmore T,Trichel A,Ortiz LA,Cole KS,Barry E.Pneumonic tularemia in rabbits resembles the human disease as illustrated by radiographic and hematological changes after infection.PLoS One. 2001;6:e24654-e24654
182. Hooper JW,Larsen T,Custer DM,Schmaljohn CS.A lethal disease model for hantavirus pulmonary syndrome.Virology. 2001;289:6-14
183. Roberts A,Paddock C,Vogel L,Butler E,Zaki S,Subbarao K.Aged BALB/c mice as a model for increased severity of severe acute respiratory syndrome in elderly humans.J Virol. 2005;79:5833-8
184. de Wit E, Rasmussen AL, Falzarano D, Bushmaker T, Feldmann F, Brining DL, Fischer ER, Martellaro C, Okumura A, Chang J, Scott D, Benecke AG, Katze MG, Feldmann H, Munster VJ. Middle east respiratory syndrome coronavirus (MERS-CoV) causes transient lower respiratory tract infection in rhesus macaques. PNAS 2013;doi:10.1073/pnas.1310744110.
185. Yen YT,Liao F,Hsiao CH,Kao CL,Chen YC,Wu-Hsieh BA.Modeling the early events of severe acute respiratory syndrome coronavirus infection in vitro.J Virol. 2006;80:2684-93
186. Zhou J, Chu H, Li C, Wong BH, Cheng ZS, Poon VK, Sun T, Lau CC, Wong KK, Chan JY, Chan JF, To KK, Chan KH, Zheng BJ, Yuen KY. Active MERS-CoV replication and aberrant induction of inflammatory cytokines and chemokines in human macrophages: implications for pathogenesis. J Infect Dis 2013;doi: 10.1093/infdis/jit504.
187. Jungraithmayr W,Draenert A,Marquardt K,Weder W.Ultrastructural Changes in acute lung allograft rejection: novel insights from an animal study.J Heart Lung Transplant. 2012;31:94-100
188. Cotran RS.American Association of Pathologists president's address. New roles for the endothelium in inflammation and immunity.Am J Pathol. 1987;129:407-13
189. Baluna R,Vitetta ES.Vascular leak syndrome: a side effect of immunotherapy.Immunopharmacology. 1997;37:117-32
190. Rosenstein M,Ettinghausen SE,Rosenberg SA.Extravasation of intravascular fluid mediated by the systemic administration of recombinant interleukin 2.J Immunol. 1986;137:1735-42
191. Conant EF,Fox KR,Miller WT.Pulmonary edema as a complication of interleukin-2 therapy.AJR Am J Roentgenol. 1989;152:749-52
192. Mossman BT,Lippmann M,Hesterberg TW,Kelsey KT,Barchowsky A,Bonner JC.Pulmonary endpoints (Lung carcinomas and asbestosis) following inhalation exposure to asbestos.J Toxicol Environ Health Part B. 2011;14:76-121
193. Ervin MH.A simple low-vacuum environmental cell.Microsc Microanal. 2003;9:18-28
194. Valberg PA,Bruch J,McCunney RJ.Are rat results from Intratracheal instillation of 19 granular dusts a reliable basis for predicting cancer risk?.Regul Toxicol Pharmacol. 2009;54:72-83
195. Veranth JM,Cutler NS,Kaser EG,Reilly CA,Yost GS.Effects of cell type and culture media on Interleukin-6 secretion in response to environmental particles.Toxicol In Vitro. 2008;22:498-509
196. Sussman RG,Cohen BS,Lippmann M.Asbestos fiber deposition in a human tracheobronchial cast.I Exp Inhal Toxicol. 1991;3:145-60
197. Seydou Y,Chen HH,Harte E,Ventura GD,Petibois C.The role of asbestos morphology on their cellular toxicity: an in vitro 3D Raman/Rayleigh imaging study.Anal Bioanal Chem. 2013;405:8701-7
198. Tsuda A,Stringer BK,Mijailovich SM,Rogers RA,Hamada K,Gray ML.Alveolar cell stretching in the presence of fibrous particles indues interleukin-8 responses.Am J Respir Cell Mol Biol. 1999;21:455-62
199. van der Worp HB,Howells DW,Sena ES,Porritt MJ,Rewell S,O’Collins V,Macleod MR.Can animal models of disease reliably inform human studies?.PLoS Med. 2010;7:e1000245-e1000245
200. Yen YT,Liao F,Hsiao CH,Kao CL,Chen YC,Wu-Hsieh BA.Modeling the early events of severe acute respiratory syndrome coronavirus infection in vitro.J Virol. 2006;80:2684-93
201. Cordes N,Blaese MA,Plasswilm L,Rodemann,Van Beuningen D.Fibronectin and laminin increase resistance to ionizing radiation and the cyotoxic drug Ukrain in human tumour and normal cells in vitro.Int J Radiat Biol. 2003;79:709-20
202. Al-Batran SE,Astner ST,Supthut M,Gamarra F,Brueckner K,Welsch U,Knuechel R,Huber RM.Three-dimensional in vitro cocultivation of lung carcinoma cells with human bronchial organ culture as a model for bronchial carcinoma.Am J Respir Cel Mol Biol. 1999;21:200-8
203. Hanahan D,Weinberg RA.The hallmarks of cancer.Cell. 2000;100:57-70
204. Kwon M,Berns A.Mouse models for lung cancer.Mol Oncol. 2013;7:165-77
205. Zheng MF,Shen SY,Huang WD.DCA increases the antitumor effects of capecitabine in a mouse B16 melanoma allograft and a human non-small cell lung cancer A549 xenograft.Cancer Chemother Pharmacol. 2013;72:1031-41
206. Pilewski JM,Bumbalo TS,Davis AG,Siegfried JM.Hepatocyte growth factor promotes tumor growth in a novel in vivo model of human lung Cancer.Am J Respir Cell Mol Biol. 2001;24:556-62
207. Moro M, Bertolini G, Tortoreto, Pastorino U, Sozzi G, Roz L. Patient-derived xenografts of non small cell lung cancer: resurgence of an old model for investigation of modern concepts of tailored therapy and cancer stem cells. J Biomed Biotechnol 2012; Article ID 568567 doi:10.1155/2012/568567.
208. Cordes N,Blaese MA,Plasswilm L,Rodemann,Van Beuningen D.Fibronectin and laminin increase resistance to ionizing radiation and the cyotoxic drug Ukrain in human tumour and normal cells in vitro.Int J Radiat Biol. 2003;79:709-20