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Volumn 100, Issue , 2014, Pages 123-130
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Direct real-time quantitative PCR for measurement of host-cell residual DNA in therapeutic proteins
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Author keywords
Biologics; CHO; Real time qPCR; Residual DNA; Therapeutic proteins
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Indexed keywords
DNA POLYMERASE;
DODECYL SULFATE SODIUM;
POLYSORBATE 20;
PROTEINASE K;
REPETITIVE DNA;
TAQ POLYMERASE;
BUFFER;
DNA;
RECOMBINANT PROTEIN;
CELL DNA;
PROTEIN;
SODIUM CHLORIDE;
THERAPEUTIC PROTEIN;
UNCLASSIFIED DRUG;
ANIMAL CELL;
ANIMAL EXPERIMENT;
ARTICLE;
CONTROLLED STUDY;
DIRECT REAL TIME QUANTITATIVE POLYMERASE CHAIN REACTION;
DNA EXTRACTION;
DNA PURIFICATION;
ESCHERICHIA COLI;
FEMALE;
HOST CELL;
INCUBATION TIME;
LIMIT OF QUANTITATION;
NONHUMAN;
POLYACRYLAMIDE GEL ELECTROPHORESIS;
PRIORITY JOURNAL;
PROTEIN AGGREGATION;
REAL TIME POLYMERASE CHAIN REACTION;
RESIDUAL HOST CELL;
ANIMAL;
BIOSYNTHESIS;
CHEMISTRY;
CHO CELL LINE;
CRICETULUS;
DRUG CONTAMINATION;
GENETIC TRANSFECTION;
GENETICS;
ISOLATION AND PURIFICATION;
METABOLISM;
MOUSE;
PH;
PROCEDURES;
REPRODUCIBILITY;
DNA DETERMINATION;
DNA ISOLATION;
ENZYME INHIBITION;
HUMAN;
PRECIPITATION;
PROTEIN DEGRADATION;
PROTEIN DETERMINATION;
ANIMALS;
BUFFERS;
CHO CELLS;
CRICETULUS;
DNA;
DRUG CONTAMINATION;
ENDOPEPTIDASE K;
ESCHERICHIA COLI;
HYDROGEN-ION CONCENTRATION;
MICE;
REAL-TIME POLYMERASE CHAIN REACTION;
RECOMBINANT PROTEINS;
REPRODUCIBILITY OF RESULTS;
SODIUM DODECYL SULFATE;
TRANSFECTION;
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EID: 84906515258
PISSN: 07317085
EISSN: 1873264X
Source Type: Journal
DOI: 10.1016/j.jpba.2014.07.032 Document Type: Article |
Times cited : (14)
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References (24)
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