Simultaneous high-resolution pH and spectrophotometric recordings of oxygen binding in blood microvolumes

Journal of Experimental Biology

Volumn 217, Issue 9, 2014, Pages 1430-1436

  Oellermann, Michael ^a   Pörtner, Hans O ^a   Mark, Felix C ^a  

^a ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESEARCH   (Germany)

Author keywords

Amphipod; Antarctic fish; Diffusion chamber; Haemocyanin; Haemoglobin; Hemocyanin; Octopus; Oxygen equilibrium curve; PH optode

Indexed keywords

HEMOCYANIN; OXYGEN;

AMPHIPODA; ANIMAL; BLOOD GAS ANALYSIS; FISH; HEMOLYMPH; METABOLISM; OCTOPUS; PH; PROCEDURES; SPECTROPHOTOMETRY;

AMPHIPODA; ANIMALS; BLOOD GAS ANALYSIS; FISHES; HEMOCYANIN; HEMOLYMPH; HYDROGEN-ION CONCENTRATION; OCTOPODIFORMES; OXYGEN; SPECTROPHOTOMETRY;

EID: 84906328121     PISSN: 00220949     EISSN: 14779145     Source Type: Journal    
DOI: 10.1242/jeb.092726     Document Type: Article

References (47)

1. Alexander, J. B. and Ingram, G. A. (1980). A comparison of five of the methods commonly used to measure protein concentrations in fish sera. J. Fish Biol. 16, 115-122.
2. Bert, P. (1878). La Pression Barométrique: Recherches de Physiologie Expérimentale. Paris: G. Masson.
3. Bohr, C., Hasselbalch, K. and Krogh, A. (1904). Uber einen in biologischer Beziehung wichtigen Einfluss, den die Kohlensäurespannung des Blutes auf dessen Sauerstoffbindung übt. Skand. Arch. Physiol. 16, 402-412.
4. Bolton, J. C., Collins, S., Smith, R., Perkins, B., Bushway, R., Bayer, R. and Vetelino, J. (2009). Spectroscopic analysis of hemolymph from the American lobster (Homarus americanus). J. Shellfish Res. 28, 905-912.
5. Bridges, C. R. (1995). Bohr and root effects in cephalopod haemocyanins-paradox or pressure in Sepia officinalis? Mar. Freshw. Behav. Physiol. 25, 121-130.
6. Bridges, C. R., Morris, S. and Grieshaber, M. K. (1984). Modulation of haemocyanin oxygen affinity in the intertidal prawn Palaemon elegans (Rathke). Respir. Physiol. 57, 189-200.
7. Brix, O. (1983). Giant squids may die when exposed to warm water currents. Nature 303, 422-423.
8. Brix, O., Bardgard, A., Cau, A., Colosimo, A., Condo, S. and Giardina, B. (1989). Oxygen-binding properties of cephalopod blood with special reference to environmental temperatures and ecological distribution. J. Exp. Zool. 252, 34-42.
9. Brix, O., Colosimo, A. and Giardina, B. (1995). Temperature dependence of oxygen binding to cephalopod haemocyanins: Ecological implications. Mar. Freshw. Behav. Physiol. 25, 149-162.
10. Chanutin, A. and Curnish, R. R. (1967). Effect of organic and inorganic phosphates on the oxygen equilibrium of human erythrocytes. Arch. Biochem. Biophys. 121, 96-102.
11. Clark, T. D., Seymour, R. S., Wells, R. M. G. and Frappell, P. B. (2008). Thermal effects on the blood respiratory properties of southern bluefin tuna, Thunnus maccoyii. Comp. Biochem. Physiol. 150A, 239-246.
12. Dickson, A. (2010). The carbon dioxide system in seawater: Equilibrium chemistry and measurements. In Guide to Best Practices for Ocean Acidification Research and Data Reporting (ed. U. Riebesell, V. J. Fabry, L. Hansson and J.-P. Gattuso). Luxembourg: Publications Office of the European Union.
13. Festa, R. S. and Asakura, T. (1979). Oxygen dissociation curves in children with anemia and malignant disease. Am. J. Hematol. 7, 233-244.
14. Herbert, N. A., Skov, P. V., Wells, R. M. G. and Steffensen, J. F. (2006). Whole blood-oxygen binding properties of four cold-temperate marine fishes: Blood affinity is independent of pH-dependent binding, routine swimming performance, and environmental hypoxia. Physiol. Biochem. Zool. 79, 909-918.
15. Houlihan, D., Innes, A., Wells, M. and Wells, J. (1982). Oxygen consumption and blood gases of Octopus vulgaris in hypoxic conditions. J. Comp. Physiol. B 148, 35-40.
16. Hüfner, G. (1890). Uber das gesetz der dissociation des oxyhaemoglobins und über einige daran sich knüpfende wichtige fragen aus der biologie. Arch. Anat. Physiol. 1, 1-27.
17. Ikeda, Y., Sugimoto, C., Yonamine, H. and Oshima, Y. (2009). Method of ethanol anaesthesia and individual marking for oval squid (Sepioteuthis lessoniana Férussac, 1831 in Lesson 1830-1831). Aquac. Res. 41, 157-160.
18. Lapennas, G. N. and Lutz, P. L. (1982). Oxygen affinity of sea turtle blood. Respir. Physiol. 48, 59-74.
19. Lapennas, G. N., Colacino, J. M. and Bonaventura, J. (1981). Thin-layer methods for determination of oxygen binding curves of hemoglobin solutions and blood. In Methods in Enzymology, Vol. 76 (ed. E. Chianconi, L. Rossi-Bernardi and E. Antonini), pp. 449-470. London: Academic Press.
20. Lykkeboe, G. and Johansen, K. (1982). A cephalopod approach to rethinking about the importance of the bohr and haldane effects. Pac. Sci. 36, 305-313.
21. Mangum, C. P. and Lykkeboe, G. (1979). The influence of inorganic ions and pH on oxygenation properties of the blood in the gastropod mollusc Busycon canaliculatum. J. Exp. Zool. 207, 417-430.
22. Meir, J. U., Champagne, C. D., Costa, D. P., Williams, C. L. and Ponganis, P. J. (2009). Extreme hypoxemic tolerance and blood oxygen depletion in diving elephant seals. Am. J. Physiol. 297, R927-R939.
23. Miller, K. I. (1985). Oxygen equilibria of Octopus dofleini hemocyanin. Biochemistry 24, 4582-4586.
24. Morris, S. and Oliver, S. (1999). Respiratory gas transport, haemocyanin function and acid-base balance in Jasus edwardsii during emersion and chilling: Simulation studies of commercial shipping methods. Comp. Biochem. Physiol. 122A, 309-321.
25. Morris, S., Taylor, A. C., Bridges, C. R. and Grieshaber, M. K. (1985). Respiratory properties of the haemolymph of the intertidal prawn Palaemon elegans (Rathke). J. Exp. Zool. 233, 175-186.
26. Niesel, W. and Thews, G. (1961). Ein neues verfahren zur schnellen und genauen aufnahme der sauerstoffbindungskurve des blutes und konzentrierter hämoproteidlösungen. Pflugers Arch. 273, 380-395.
27. Olson, K. N., Hillyer, M. A., Kloss, J. S., Geiselhart, R. J. and Apple, F. S. (2010). Accident or arson: Is CO-oximetry reliable for carboxyhemoglobin measurement postmortem? Clin. Chem. 56, 515-519.
28. Pörtner, H. O. (1990). An analysis of the effects of pH on oxygen binding by squid (Illex illecebrosus, Loligo pealei) haemocyanin. J. Exp. Biol. 150, 407-424.
29. PreSens (2004). Instruction Manual pH-4 mini. Regensburg, Germany: Precision Sensing GmbH.
30. PreSens (2012). Product Sheet for pH Microsensors. Regensburg, Germany: Precision Sensing GmbH.
31. R Development Core Team (2013). R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing.
32. Rasmussen, J. R., Wells, R. M. G., Henty, K., Clark, T. D. and Brittain, T. (2009). Characterization of the hemoglobins of the Australian lungfish Neoceratodus forsteri (Krefft). Comp. Biochem. Physiol. 152A, 162-167.
33. Reeves, R. B. (1980). A rapid micro method for obtaining oxygen equilibrium curves on whole blood. Respir. Physiol. 42, 299-315.
34. Ritz, C. and Streibig, J. C. (2005). Bioassay analysis using R. J. Stat. Softw. 12, 1-22.
35. Safavi, A. and Bagheri, M. (2003). Novel optical pH sensor for high and low pH values. Sens. Actuators B Chem. 90, 143-150.
36. Scott, G. R. (2011). Elevated performance: The unique physiology of birds that fly at high altitudes. J. Exp. Biol. 214, 2455-2462.
37. Seibel, B., Chausson, F., Lallier, F., Zal, F. and Childress, J. (1999). Vampire blood: Respiratory physiology of the vampire squid (Cephalopoda: Vampyromorpha) in relation to the oxygen minimum layer. Exp. Biol. Online 4, 1-10.
38. Sick, H. and Gersonde, K. (1969). Method for continuous registration of O2-binding curves of hemoproteins by means of a diffusion chamber. Anal. Biochem. 32, 362-376.
39. Sick, H. and Gersonde, K. (1972). Theory and application of the diffusion technique for measurement and analysis of O2-binding properties of very autoxidizable hemoproteins. Anal. Biochem. 47, 46-56.
40. Truchot, J. P. (1976). Carbon dioxide combining properties of the blood of the shore crab, Carcinus maenas (L.): CO2-dissociation curves and Haldane effect. J. Comp. Physiol. 112, 283-293.
41. van Holde, K. E., Miller, K. I. and Decker, H. (2001). Hemocyanins and invertebrate evolution. J. Biol. Chem. 276, 15563-15566.
42. Weber, R. E., Behrens, J. W., Malte, H. and Fago, A. (2008). Thermodynamics of oxygenation-linked proton and lactate binding govern the temperature sensitivity of O2 binding in crustacean (Carcinus maenas) hemocyanin. J. Exp. Biol. 211, 1057-1062.
43. Weber, R. E., Campbell, K. L., Fago, A., Malte, H. and Jensen, F. B. (2010). ATPinduced temperature independence of hemoglobin-O2 affinity in heterothermic billfish. J. Exp. Biol. 213, 1579-1585.
44. Wells, M. and Smith, P. (1987). The performance of the octopus circulatory system: A triumph of engineering over design. Experientia 43, 487-499.
45. Wells, R. M. G. and Weber, R. E. (1989). The measurement of oxygen affinity in blood and haemoglobin solutions. In Techniques in Comparative Respiratory Physiology: An Experimental Approach (ed. C. R. Bridges and P. J. Butler), pp. 279-303. Cambridge: Cambridge University Press.
46. Wirkner, C. S. and Richter, S. (2013). Circulatory system and respiration. The Natural History of the Crustacea 1, 376-412.
47. Zielinski, S., Sartoris, F. J. and Pörtner, H. O. (2001). Temperature effects on hemocyanin oxygen binding in an antarctic cephalopod. Biol. Bull. 200, 67-76.