Tb927.10.6900 encodes the glucosyltransferase involved in synthesis of base J in Trypanosoma brucei

Molecular and Biochemical Parasitology

Volumn 196, Issue 1, 2014, Pages 9-11

  Sekar, Aarthi ^a   Merritt, Christopher ^a   Baugh, Loren ^a   Stuart, Kenneth ^a,c   Myler, Peter J ^a,b,c  

^a SEATTLE BIOMEDICAL RESEARCH INSTITUTE   (United States)

^b Department of Biomedical Informatics and Medical Education ^*  (United States)

^c UNIVERSITY OF WASHINGTON   (United States)

Author keywords

DNA modification; Epigenetics; Glucosyltransferase; Transcriptional read through; Trypanosomatids

Indexed keywords

GLUCOSYLTRANSFERASE; THYMINE;

ANALOGS AND DERIVATIVES; ENZYMOLOGY; GENE DELETION; GENETICS; METABOLISM; TRYPANOSOMA BRUCEI BRUCEI;

GENE DELETION; GLUCOSYLTRANSFERASES; METABOLIC NETWORKS AND PATHWAYS; THYMINE; TRYPANOSOMA BRUCEI BRUCEI;

EID: 84906170661     PISSN: 01666851     EISSN: 18729428     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2014.07.005     Document Type: Article

References (21)

1. F. van Leeuwen, M.C. Taylor, A. Mondragon, H. Moreau, W. Gibson, and R. Kieft et al. β-d-Glucosyl-hydroxymethyluracil is a conserved DNA modification in kinetoplastid protozoans and is abundant in their telomeres Proc Nat Acad Sci USA 95 1998 2366 2371
2. J.H. Gommers-Ampt, F. van Leeuwen, A.L. de Beer, J.F. Vliegenthart, M. Dizdaroglu, and J.A. Kowalak et al. β-d-Glucosylhydroxymethyluracil: a novel modified base present in the DNA of the parasitic protozoan T. brucei Cell 75 1993 1129 1136
3. F. van Leeuwen, E.R. Wijsman, E. Kuyl-Yeheskiely, G.A. van der Marel, J.H. van Boom, and P. Borst The telomeric GGGTTA repeats of Trypanosoma brucei contain the hypermodified base J in both strands Nucleic Acids Res 24 1996 2476 2482
4. F. van Leeuwen, E.R. Wijsman, R. Kieft, G.A. van der Marel, J.H. van Boom, and P. Borst Localization of the modified base J in telomeric VSG gene expression sites of Trypanosoma brucei Genes Dev 11 1997 3232 3241
5. F. van Leeuwen, R. Kieft, M. Cross, and P. Borst Tandemly repeated DNA is a target for the partial replacement of thymine by β-d-glucosyl- hydroxymethyluracil in Trypanosoma brucei Mol Biochem Parasitol 109 2000 133 145
6. L.J. Cliffe, T.N. Siegel, M. Marshall, G.A. Cross, and R. Sabatini Two thymidine hydroxylases differentially regulate the formation of glucosylated DNA at regions flanking polymerase II polycistronic transcription units throughout the genome of Trypanosoma brucei Nucleic Acids Res 38 2010 3923 3935
7. H.G. van Luenen, C. Farris, S. Jan, P.A. Genest, P. Tripathi, and A. Velds et al. Glucosylated hydroxymethyluracil, DNA base J, prevents transcriptional readthrough in Leishmania Cell 150 2012 909 921
8. A. Bernards, N. Harten-Loosbroek, and P. Borst Modification of telomeric DNA in Trypanosoma brucei; a role in antigenic variation? Nucleic Acids Res 12 1984 4153 4170
9. E. Pays, M.F. Delauw, M. Laurent, and M. Steinert Possible DNA modification in GC dinucleotides of Trypanosoma brucei telomeric sequences; relationship with antigen gene transcription Nucleic Acids Res 12 1984 5235 5247
10. P. Borst, and R. Sabatini Base J: discovery, biosynthesis, and possible functions Annu Rev Microbiol 62 2008 235 251
11. L.J. Cliffe, R. Kieft, T. Southern, S.R. Birkeland, M. Marshall, and K. Sweeney et al. JBP1 and JBP2 are two distinct thymidine hydroxylases involved in J biosynthesis in genomic DNA of African trypanosomes Nucleic Acids Res 37 2009 1452 1462
12. L.M. Iyer, M. Tahiliani, A. Rao, and L. Aravind Prediction of novel families of enzymes involved in oxidative and other complex modifications of bases in nucleic acids Cell Cycle 8 2009 1698 1710
13. L.M. Iyer, D. Zhang, A. Maxwell Burroughs, and L. Aravind Computational identification of novel biochemical systems involved in oxidation, glycosylation and other complex modifications of bases in DNA Nucleic Acids Res 41 2013 7635 7655
14. C. Merritt, and K. Stuart Identification of essential and non-essential protein kinases by a fusion PCR method for efficient production of transgenic Trypanosoma brucei Mol Biochem Parasitol 19 2013 44 49
15. E. Wirtz, S. Leal, C. Ochatt, and G.A. Cross A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei Mol Biochem Parasitol 99 1999 89 101
16. P.A. Genest, B. ter Riet, T. Cijsuow, H. van Luenen, and P. Borst Telomeric localization of modified DNA base J in the genome of the protozoan parasite Leishmania Nucleic Acids Res 35 2007 2116 2124
17. B.C. Jensen, D. Sivam, C.T. Kifer, P.J. Myler, and P. Parsons Widespread variation in transcript abundance within and across developmental stages of Trypanosoma brucei BMC Genomics 10 2009 482
18. T.N. Siegel, D.R. Hekstra, X. Wang, S. Dewell, and G.A. Cross Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites Nucleic Acids Res 38 2010 4946 4957
19. D. Nilsson, K. Gunasekera, J. Mani, M. Osteras, L. Farinelli, and L. Baerlocher et al. Spliced leader trapping reveals widespread alternative splicing patterns in the highly dynamic transcriptome of Trypanosoma brucei PLoS Pathog 6 2010 e1001037
20. J.D. Thompson, D.G. Higgins, and T.J. Gibson CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucleic Acids Res 22 1994 4673 4680
21. W. Bullard, J. Lopes da Rosa-Spiegler, S. Liu, Y. Wang, and R. Sabatini Identification of the glucosyltransferase that converts hydroxymethyluracil to base J in the trypanosomatid genome J Biol Chem 2014 10.1074/jbc.M114.579821