Development and Integration of Quantitative Real-Time PCR Methods for Detection of Mitochondrial DNA and Methanobrevibacter smithii nifH Gene as Novel Microbial Source Tracking Tools

Environmental Forensics

Volumn 15, Issue 3, 2014, Pages 256-264

  Dancer, Daniel ^a,b   Baker Austin, Craig ^a   Lowther, James A ^a   Hartnell, Rachel E ^a   Lees, David N ^a   Roberts, L O ^b  

^a CENTRE FOR ENVIRONMENT   (United Kingdom)

^b UNIVERSITY OF SURREY   (United Kingdom)

Author keywords

Microbial source tracking; mitochondrial DNA; quantitative real time polymerase chain reaction (qPCR)

Indexed keywords

EID: 84906088292     PISSN: 15275922     EISSN: 15275930     Source Type: Journal    
DOI: 10.1080/15275922.2014.930762     Document Type: Article

References (20)

1. Albaugh, G. P., Iyengar, V., Lohani, A., Malayeri, M., Bala, S., and Nair, P. P. 1992. Isolation of exfoliated colonic epithelial-cells, a novel, noninvasive approach to the study of cellular markers. International Journal of Cancer 52:347-350.
2. Andreasson, H., Gyllensten, U., and Allen, M. 2002. Real-time DNA quantification of nuclear and mitochondrial DNA in forensic analysis. Biotechniques 33:407-411.
3. Baker-Austin, C., Rangdale, R., Lowther, J., and Lees, D. N. 2010. Mitochondrial DNA analysis in microbial source tracking in shellfish waters. Water Science and Technology 61:1-7.
4. Caldwell, J. M., Raley, M. E., and Levine, J. F. 2007 Mitochondrial multiplex real-time PCR as a source tracking method in fecal-contaminated effluents. Environmental Science and Technology 41:3277-3283.
5. Cox, P., Griffith, M., Angles, Deere, D., and Ferguson, C. 2005. Concentrations of pathogens and indicators in animal faeces in the Sydney watershed. Applied Environmental Microbiology 71:5929-2934.
6. Dorfman, M., and Sinclair Rosselot, K. 2008. Testing the Waters 2008: A Guide to Water Quality Testing at Vacation Beaches. Washington, DC: Natural Resources Defence Council.
7. European Communities. 2004, July 25. Regulation (EC) No 854/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific rules for the organisation of official controls on products of animal origin intended for human consumption. Official Journal of the European Communities, L226:83-127.
8. European Communities. 2006, February 15. Management of bathing water quality and repealing, Directive 76/160/EEC. European Parliament and Council, 2006/7/EC.
9. Feldhusen, F. 2000. The role of seafood in bacterial foodborne disease. Microbes and Infection 2:1651-1660.
10. Field, K. G., and Samadpour, M. 2007. Fecal source tracking, the indicator paradigm, and managing water quality. Water Research 41:3517-3538.
11. Fong, T. T., and Lipp, E. K. 2005. Enteric viruses of humans and animals in aquatic environments: Health risks, detection and detection, and potential water quality assessment tools, Microbiology and Molecular Biology Reviews 69:357-371.
12. Gentry-Shields, J., Rowny, J. G., and Stewart, J. R. 2012. HuBac and nifH source tracking markers display a relationship to land use but not rainfall. Water Research 46:6163-6174.
13. Gordon, D. M. 2001. Geographical structure and host specificity in bacteria and implications for tracing the source of coliform contamination. Microbiology 147:1079-1085.
14. Kortbaoui, R., Locas, A., Imbeau, M., Payment, P., and Villemur, R. 2009. Universal mitochondrial PCR combined with species specific dot-blot assay as a source-tracking method of human, bovine, chicken, ovine, and porcine in fecal-contaminated surface water. Water Research 43:2002-2010.
15. Martellini, A., Payment, P., and Villemur, R. 2005. Use of eukaryotic mitochondrial DNA to differentiate human, bovine, porcine and ovine sources in fecally contaminated surface water. Water Research 39:541-548.
16. Rabinovici, S. J., Bernknopf, R. L., Wein, A. M., Coursey, D. L., and Whitman, R. L. 2004. Economic and health risk trade-offs of swim closures at Lake Michigan beach, Environmental Science and Technology 38:2737-2745.
17. Roslev, P., and Bukh, A. S. 2011. State of the art molecular markers for fecal pollution source tracking in water. Applied Microbiology Biotechnology 89:1341-1355.
18. Schill, W. B., and Mathes, M. V. 2008. Real-time PCR detection and quantification of nine potential sources of fecal contamination by analysis of mitochondrial cytochrome-b targets, Environmental Science and Technology 42:5229-5234.
19. Stewart, J. R., Santa Domingo, J. W., and Wade, T. J. 2007. Fecal pollution, public health, and microbial source tracking. In Microbial Source Tracking, eds., Santa Domingo, J. W., and M. J. Sadowsky. Washington, DC: ASM Press, 1-32.
20. Ufnar, J. A., Wang, S. Y., Christiansen, J. M., Yampara-Iquise, H., Carson, C. A., and Ellender, R. D. 2010. Detection of the nifH gene of Methanobrevibacter smithii: A potential tool to identify sewage pollution in recreational waters. Journal of Applied Microbiology 101:44-52.