Fast procedure for the analysis of poly(hydroxyalkanoates) in bacterial cells by off-line pyrolysis/gas-chromatography with flame ionization detector

Journal of Chromatography A

Volumn 1359, Issue , 2014, Pages 230-236

  Torri, Cristian ^a   Cordiani, Helena ^a   Samorì, Chiara ^a   Favaro, Lorenzo ^b   Fabbri, Daniele ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

Crotonic acid; Green analytical chemistry; Microbial mixed consortia; Polyhydroxyalkanoates; Pyrolysis; Quantification

Indexed keywords

CHLORINE COMPOUNDS; GAS CHROMATOGRAPHY; IONIZATION OF GASES; MASS SPECTROMETRY; MONOMERS; ORGANIC SOLVENTS; POLYMERS;

CROTONIC ACIDS; GREEN ANALYTICAL CHEMISTRY; MICROBIAL MIXED CONSORTIA; POLYHYDROXYALKANOATES; QUANTIFICATION;

PYROLYSIS;

2 ALKENOIC ACID; ALKENE DERIVATIVE; CHLOROFORM; CROTONIC ACID DERIVATIVE; HYDROXYBUTYRIC ACID; MONOMER; POLY(3 HYDROXYBUTANOATE CO 3 HYDROXYCAPROATE); POLY(3 HYDROXYBUTANOATE CO 3 HYDROXYVALERATE); POLY(3 HYDROXYBUTYRIC ACID); POLYHYDROXYALKANOIC ACID; SOLVENT; SULFURIC ACID; UNCLASSIFIED DRUG;

ALCOHOLYSIS; ANALYTICAL EQUIPMENT; ARTICLE; BACTERIAL CELL; BIOMASS; CHEMICAL ANALYSIS; CONTROLLED STUDY; CUPRIAVIDUS NECATOR; FLAME IONIZATION DETECTOR; GREEN CHEMISTRY; INFRARED SPECTROSCOPY; LIMIT OF DETECTION; MASS FRAGMENTOGRAPHY; METHANOLYSIS; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PYROLYSIS; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; RELIABILITY;

BACTERIA (MICROORGANISMS);

CROTONIC ACID; GREEN ANALYTICAL CHEMISTRY; MICROBIAL MIXED CONSORTIA; POLYHYDROXYALKANOATES; PYROLYSIS; QUANTIFICATION;

3-HYDROXYBUTYRIC ACID; BIOMASS; BIOPOLYMERS; CAPROATES; CUPRIAVIDUS NECATOR; FLAME IONIZATION; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HYDROXYBUTYRATES; POLYESTERS;

EID: 84906058624     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.07.008     Document Type: Article

References (27)

1. Ottaway J.H. A preparation of d(-)-β-hydroxybutyric acid. Biochem. J 1962, 84:11.
2. Braunegg G., Sonnleitner B., Lafferty R.M. A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. Biotechnol. 1978, 6:29-37.
3. Baetens D., Aurola A.M., Foglia A., Dionisi D., van Loosdrecht M.C.M. Gas chromatographic analysis of polyhydroxybutyrate in activated sludge: a round-robin test. Water Sci. Technol. 2002, 46:357-361.
4. Gałuszka A., Migaszewski Z., Namieśnik J. The 12 principles of green analytical chemistry and the significance mnemonic of green analytical practices. Trends Anal. Chem. 2013, 50:78-84.
5. Werker A., Lind P., Bengtsson S., Nordström F. Chlorinated-solvent-free gas chromatographic analysis of biomass containing polyhydroxyalkanoates. Water Res. 2008, 42:2517-2526.
6. Hahn S.K., Chang Y.K. A thermogravimetric analysis for poly(3-hydroxybutyrate) quantification. Biotechnol. Tech. 1995, 9:873-878.
7. Hong K., Sun S., Tian W., Chen G.Q., Huang W. A rapid method for detecting bacterial polyhydroxyalkanoates in intact cells by Fourier transform infrared spectroscopy. Appl. Microbiol. Biotechnol. 1999, 51:523-526.
8. Arcos-Hernandeza M.V., Gurieff N., Pratt S., Magnusson P., Werker A., Vargas A., Lant P. Rapid quantification of intracellular PHA using infrared spectroscopy: an application in mixed cultures. J. Biotechnol. 2010, 150:372-379.
9. Jarute G., Kainz A., Schroll G., Baena J.R., Lendl B. On-line determination of the intracellular poly(beta-hydroxybutyric acid) content in transformed Escherichia coli and glucose during PHB production using stopped-flow attenuated total reflection FT-IR spectrometry. Anal. Chem. 2004, 76:6353-6358.
10. Zuriani R., Vigneswari S., Azizan M.N.M., Majid M.I.A., Amirul A.A. A high throughput Nile Red fluorescence method for rapid quantification of intracellular bacterial polyhydroxyalkanoates. Biotechnol. Bioproc. Eng. 2013, 18:472-478.
11. Li S.-D., He J.-D., Yu P.H., Cheung M.K. Thermal degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) as studied by TG, TG-FTIR, and Py-GC/MS. J. Appl. Polym. Sci. 2003, 89:1530-1536.
12. Morikawa H., Marchessault R. Pyrolysis of bacterial polyalkanoates. Can. J. Chem. 1981, 59:2306-2313.
13. Aoyagia Y., Yamashita K., Doi Y. Thermal degradation of poly[(R)-3-hydroxybutyrate], poly[e-caprolactone], and poly[(S)-lactide]. Polym. Degrad. Stab. 2002, 76:53-59.
14. http://www.sigmaaldrich.com/etc/medialib/docs/Supelco/General_Information/t407133.Par.0001.File.tmp/t407133.pdf.
15. Sato S.H., Hoshino M., Aoi H., Seino T., Ishida Y., Aoi K., Ohtani H. Compositional analysis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by pyrolysis-gas chromatography in the presence of organic alkali. J. Anal. Appl. Pyrolysis 2005, 74:193-199.
16. Fabbri D., Baravelli V., Chiavari G., Prati S., Finessi E. The influence of nanopowder metal oxides on the methylating activity of dimethyl carbonate in analytical pyrolysis. J. Anal. Appl. Pyrolysis 2007, 79:2-8.
17. Kim K.J., Doi Y., Abe H. Effects of residual metal compounds and chain-end structure on thermal degradation of poly(3-hydroxybutyric acid). Polym. Degrad. Stab. 2006, 91:769-777.
18. Kim K.J., Doi Y., Abe H. Effect of metal compounds on thermal degradation behavior of aliphatic poly(hydroxyalkanoic acid)s. Polym. Degrad. Stab. 2008, 93:776-785.
19. Watt B.E., Morgan S.L. 2-Butenoic acid, a chemical marker for poly-β-hydroxybutyrate identified by pyrolysis-gas chromatography/mass spectrometry in analyses of whole microbial cells. J. Anal. Appl. Pyrolysis 1991, 19:237-249.
20. Torri C., Adamiano A., Fabbri D., Lindfors C., Monti A., Oasmaa A. Comparative analysis of pyrolysate from herbaceous and woody energy crops by Py-GC with atomic emission and mass spectrometric detection. J. Anal. Appl. Pyrolysis 2010, 88:175-180.
21. Lemos P.C., Serafim L.S., Reis M.A.M. Synthesis of polyhydroxyalkanoates from different short-chain fatty acids by mixed cultures submitted to aerobic dynamic feeding. J. Biotechnol. 2006, 122:226-238.
22. Povolo S., Toffano P., Basaglia M., Casella S. Polyhydroxyalkanoates production by engineered Cupriavidus necator from waste material containing lactose. Bioresour. Technol. 2010, 101:7902-7907.
23. Fabbri D., Torri C., Baravelli V. Effect of zeolites and nanopowder metal oxides on the distribution of chiral anhydrosugars evolved from pyrolysis of cellulose: an analytical study. J. Anal. Appl. Pyrolysis 2007, 80:24-29.
24. Gonzalez A., Irusta L., Fernandez-Berridi M.J., Iriarte M., Iruin J.J. Application of pyrolysis/gas chromatography/Fourier transform infrared spectroscopy and TGA techniques in the study of thermal degradation of poly (3-hydroxybutyrate). Polym. Degrad. Stab. 2005, 87:347e354.
25. Kopinke F.D., Mackenzie K. Mechanistic aspects of the thermal degradation of poly(lactic acid) and poly(β-hydroxybutyric acid). J. Anal. Appl. Pyrolysis 1997, 40/41:43-53.
26. Mullen C., Boateng A.A., Schweitzer D., Sparks K., Snell K.D. Mild pyrolysis of P3HB/switchgrass blends for the production of bio-oil enriched with crotonic acid. J. Anal. Appl. Pyrolysis 2014, 107:40-45.
27. Patwardhan P.R., Satrio J.A., Brown R.C., Shanks B.H. Influence of inorganic salts on the primary pyrolysis products of cellulose. Bioresour. Technol. 2010, 101:4646-4655.